Your Good Partner in Biology Research

  1. 首页
  2. 蛋白
  3. 重组蛋白
  4. Recombinant Rat Erythropoietin (Epo)

Recombinant Rat Erythropoietin (Epo)

  • 货号：
    CSB-YP007743RA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP007743RA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP007743RA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP007743RA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP007743RA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    EpoErythropoietin
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    27-192
  • 氨基酸序列
    APPR LICDSRVLER YILEAKEAEN VTMGCAEGPR LSENITVPDT KVNFYAWKRM KVEEQAVEVW QGLSLLSEAI LQAQALQANS SQPPESLQLH IDKAISGLRS LTSLLRVLGA QKELMSPPDA TQAAPLRTLT ADTFCKLFRV YSNFLRGKLK LYTGEACRRG DR
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Hormone involved in the regulation of erythrocyte proliferation and differentiation and the maintenance of a physiological level of circulating erythrocyte mass. Binds to EPOR leading to EPOR dimerization and JAK2 activation thereby activating specific downstream effectors, including STAT1 and STAT3.
  • 基因功能参考文献：
    1. Increases in plasma erythropoietin and erythropoietin receptor activation are mechanisms implicated in the increase of plasma FGF23 in acute kidney injury. PMID: 29395333
    2. Epo and Epo-Receptor are expressed in the spiral ganglion neurons (SGNs) of the inner ear of the rat, and Epo adenovirus vector (AdEpo) can decrease the spontaneous apoptosis of SGNs, which may provide a basis for the prevention or alleviation of sensorineural hearing loss. PMID: 29436578
    3. EPO administration increased TMPRSS6 content in the plasma membrane-enriched fraction of liver homogenate; in iron-pretreated rats, this increase was abolished. The results confirm that iron pretreatment prevents the EPO-induced decrease in liver Hamp expression. PMID: 28282554
    4. Protection of erythropoietin markedly attenuated pyroptosis and apoptosis of neurons exposed to sevoflurane via Erk1/2-Nrf2/Bach1 signal pathway. PMID: 28189515
    5. protective effects of EPO may be mediated by nitric oxide and the expression of different NOS enzymes, especially iNOS isoform PMID: 28087117
    6. EPO receptor ligand dimeric peptides AF41676 and AF43136 administered before reperfusion are able to reduce infarct size in a rat model of AMI. PMID: 27521836
    7. The present study aimed to determine the expression of erythropoietin (EPO) and the EPO receptor (EPOR) in spiral ganglion neurons (SGNs) in the inner ear of rats of various ages, and the associated neuronal apoptosis and hearing alterations. PMID: 27959434
    8. Study shows that erythropoietin reduced chemokine CCL3 mRNA expression in rat cerebellar neuron-enriched preparations during neuroinflammation. PMID: 26178913
    9. Taken together, our results reveal that EPO and cEPO mediate MEF2 target gene expression via the regulation of HDAC5 phosphorylation at Ser259/498, and suggest that HDAC5 could be a mechanism contributing to the therapeutic actions of EPO and cEPO. PMID: 26777998
    10. this study is the first to elucidate the detailed mechanism by which AhR plays an indispensable role in the suppression of HIF activation by IS. PMID: 26561638
    11. the hypercapnic hypoxia causes maximum activation of the synthesis and accumulation of erythropoietin HIF-1alpha, and permissive hypercapnia enhances their content more than hypoxic exposure. PMID: 26852593
    12. Results of the present study demonstrate the neuroprotective, anti-inflammatory and antioxidant effects of EPO in a rotenone-induced neurodegenerative animal model. PMID: 25464888
    13. Vitamin A deficiency leads to ineffective erythropoiesis by the down-regulation of renal erythropoietin expression in the kidney, resulting in erythrocyte malformation and the consequent accumulation of the heme group in the spleen. PMID: 24998947
    14. EPO partially rescued insulin-stimulated AKT activation, reduced oxidative stress, and restored heat-shock protein 72 expression in soleus muscles. PMID: 25767056
    15. Results showed that the inhibitory effects of TNFa on the hypoxia-induced upregulation of erythropoietin expression are mediated primarily by HIF-2a rather than HIF-1a PMID: 25283246
    16. Data suggest a potential positive effect of erythropoietin (EPO) on hepatocytes (HCs) proliferation during liver regeneration after a partial hepatectomy. PMID: 24928528
    17. HIF-1alpha/EPO hypoxic signal transduction may initiate hippocampal neurogenesis following hypoxia-ischemia PMID: 25935977
    18. The present study suggests that EPO can exert a cardioprotective effect on DOX-induced cardiotoxicity which may be associated with improving MMP-2/TIMP-2 imbalance. PMID: 24685074
    19. Epidermal growth factor and erythropoietin infusion accelerate functional recovery in combination with rehabilitation. PMID: 24763928
    20. In IN-IC cells, hypoxia increased mRNA expression of erythropoietin, erythropoietin concentration in the medium and protein expression of HIF PHD2. PMID: 24832733
    21. After 2-weeks of hypoxia, erythropoietin transcripts and erythropoietin plasma levels were significantly increased in hypoxic animals compared with control animals. PMID: 24524370
    22. rMSCs transplantation could be a new therapy for degenerative retinal diseases since it can protect and rescue RPE and retinal neurons, while EPO gene modification to rMSCs could be an even better option. PMID: 23971737
    23. Erythropoietin prevents lymphoid apoptosis but has no effect on survival in experimental sepsis. PMID: 23728385
    24. EPO induces the expression of myelin genes in oligodendrocytes and this effect requires the presence of EPOR. PMID: 23821361
    25. EPO significantly suppresses the enhanced apoptosis and activity of caspase 3 that are induced by 6-hydroxydopamine. PMID: 22294054
    26. 11 weeks of endurance training increased erythrocyte deformability. Epo and EpoR may contribute to the decreased morphological index and deformability indicator in erythrocytes during endurance training in rats. PMID: 22495316
    27. EPO potently protects against cardiopulmonary bypass-induced acute renal injury by inhibiting expression of NF-kappaB p65 and inflammatory response. PMID: 22659586
    28. EPO induces a significant decrement in the ohmic airway resistance. PMID: 22766248
    29. Pyruvate activation of the hypoxia-inducible factor-1alpha-EPO signaling cascade in neurons and glia could protect the brain from ischemia-reperfusion injury PMID: 22282883
    30. Ligustilide exerts neuroprotective effects against I/R injury by promoting EPO transcription via an ERK signalling pathway and inhibiting RTP801 expression. PMID: 21410687
    31. A protective cascade for erythropoietin (EPO) relies upon modulation of Wnt1, Akt1, FoxO3a, GSK-3beta, beta-catenin, and mitochondrial apoptotic pathways for the development of new strategies against DM vascular complications. PMID: 21443457
    32. Our work identifies novel pathways for EPO in the vascular system that can govern the activity of SIRT1 PMID: 21722091
    33. EPO, hCG, or their combination ameliorate cardiac remodeling post-myocardial infarct in a rat model PMID: 21707946
    34. Epo combined with granulocyte colonystimulating factor enhanced Mmp2 expression in mesenchymal stem cells, promoted motility and activated the ERK1/2 signaling pathway. PMID: 21461559
    35. EPO mediates brain-derived neurotrophic factor-dependent sonic hedgehog (SHH) expression and 3-nitropropionic acid (3-NP) resistance because suppression of EPO activity by soluble EPO-receptor abolishes BDNF-mediated SHH induction and 3-NP resistance. PMID: 20580927
    36. The expression of erythropoietin in the hippocampus increased from 3 to 18 months and decreased from 18 to 30 months. PMID: 19779256
    37. EPO may play a role in normal spinal cord development by regulating cell proliferation and apoptosis. PMID: 20167254
    38. Changes in EPO concentration [EPO] was evaluated every hour after single exposure of 10% O(2) for 120 min in order to determine a peak level of [EPO]. PMID: 20204781
    39. Intermittent hypoxic exposure can enhance serum hypoxia inducible factor-1 alpha and erythropointin levels. PMID: 20104680
    40. Our results support a model in which Epo promotes Schwann cell migration and assembly of the provisional extracellular matrix in the injured peripheral nerve by its effects on integrin recruitment to the cell surface and local FN production. PMID: 19705458
    41. All regions of kidney express erythropoietin receptors. Review. PMID: 12381912
    42. role in preventing the induction of Apaf-1 and preserving mitochondrial membrane potential in conjunction with enhanced Bcl-XL expression. EPO also inhibits the activation of caspase-9. PMID: 12621307
    43. EPO inhibits apoptosis in ventricular myocytes exposed to hypoxia, through an Akt-dependent pathway PMID: 12927817
    44. Erythropoietin may provide benefit after hypoxic-ischemic events in the developing brain, a major contributor to static encephalopathy and cerebral palsy. PMID: 13129593
    45. Hypoxic acclimatization can inhibit the increment of erythropoietin gene expression induced by acute hypoxia in rat hepatic and renal tissues. PMID: 14706194
    46. EPO mediates the classic neurotrophic effects of proliferation, differentiation and maintenance in a dopaminergic cell line. PMID: 15050726
    47. Treatment of neural progenitor cells with erythropoietin stimulated progenitor cell differentiation into neurons, but not astrocytes. PMID: 15167538
    48. These data demonstrate that EPO can promote differentiation of neuronal stem cells into astrocytes in an EPO receptor dependent manner, and this effect may be associated with the activation of ERK kinase and NF-kappaB pathway. PMID: 15249201
    49. Age-related decreases in Erythropoietin may contribute to degenerative events following age-related decreases in brain flow and oxygen supply. PMID: 15262216
    50. Study demonstrates how in response to axonal injury, rat periaxonal Schwann cells release erythropoietin (EPO), which via EPO receptor binding on neurons, prevents axonal degeneration. PMID: 15470751

    显示更多

    收起更多

  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    EPO/TPO family
  • 组织特异性：
    Produced by kidney or liver of adult mammals and by liver of fetal or neonatal mammals.
  • 数据库链接：

    KEGG: rno:24335

    STRING: 10116.ENSRNOP00000001914

    UniGene: Rn.11365