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  4. Recombinant Rat Epoxide hydrolase 2 (Ephx2)

Recombinant Rat Epoxide hydrolase 2 (Ephx2)

  • 货号：
    CSB-YP007735RA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP007735RA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP007735RA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP007735RA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP007735RA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Ephx2
  • Uniprot No.：
  • 别名：
    Ephx2; Bifunctional epoxide hydrolase 2 [Includes: Cytosolic epoxide hydrolase 2; CEH; EC 3.3.2.10; Epoxide hydratase; Soluble epoxide hydrolase; SEH); Lipid-phosphate phosphatase; EC 3.1.3.76)]
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-554
  • 氨基酸序列
    MALRVAAFDL DGVLALPSIA GVLRHTEEAL ALPRDFLLGA FQMKFPEGPT EQLMKGKITF SQWVPLMDES CRKSSKACGA SLPENFSISE IFSQAMAARS INRPMLQAAA ALKKKGFTTC IVTNNWLDDS DKRDILAQMM CELSQHFDFL IESCQVGMIK PEPQIYKFVL DTLKAKPNEV VFLDDFGSNL KPARDMGMVT ILVRDTASAL RELEKVTGTQ FPEAPLPVPC SPNDVSHGYV TVKPGIRLHF VEMGSGPAIC LCHGFPESWF SWRYQIPALA QAGFRVLAID MKGYGDSSSP PEIEEYAMEL LCEEMVTFLN KLGIPQAVFI GHDWAGVLVW NMALFHPERV RAVASLNTPL MPPNPEVSPM EVIRSIPVFN YQLYFQEPGV AEAELEKNMS RTFKSFFRTS DDMGLLTVNK ATEMGGILVG TPEDPKVSKI TTEEEIEYYI QQFKKSGFRG PLNWYRNTER NWKWSCKALG RKILVPALMV TAEKDIVLRP EMSKNMENWI PFLKRGHIED CGHWTQIEKP AEVNQILIKW LKTEIQNPSV TSKI
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Bifunctional enzyme. The C-terminal domain has epoxide hydrolase activity and acts on epoxides (alkene oxides, oxiranes) and arene oxides. Plays a role in xenobiotic metabolism by degrading potentially toxic epoxides. Also determines steady-state levels of physiological mediators. The N-terminal domain has lipid phosphatase activity, with the highest activity towards threo-9,10-phosphonooxy-hydroxy-octadecanoic acid, followed by erythro-9,10-phosphonooxy-hydroxy-octadecanoic acid, 12-phosphonooxy-octadec-9Z-enoic acid and 12-phosphonooxy-octadec-9E-enoic acid.; Bifunctional enzyme. The C-terminal domain has epoxide hydrolase activity and acts on epoxides (alkene oxides, oxiranes) and arene oxides. Plays a role in xenobiotic metabolism by degrading potentially toxic epoxides. Also determines steady-state levels of physiological mediators.; Bifunctional enzyme. The N-terminal domain has lipid phosphatase activity, with the highest activity towards threo-9,10-phosphonooxy-hydroxy-octadecanoic acid, followed by erythro-9,10-phosphonooxy-hydroxy-octadecanoic acid, 12-phosphonooxy-octadec-9Z-enoic acid and 12-phosphonooxy-octadec-9E-enoic acid. Has phosphatase activity toward lyso-glycerophospholipids with also some lower activity toward lysolipids of sphingolipid and isoprenoid phosphates.
  • 基因功能参考文献：
    1. Data suggest that significant dietary potassium (here, even as a single meal) increases postprandial effects that reduce sEH activity in blood by mechanisms involving insulin secretion and gut microbiota; these mechanisms combat oxidative stress and prevent cardiovascular diseases. PMID: 28863368
    2. Prophylactic treatment with sEH inhibitors decreased myocardial damage due to ischemia reperfusion, hypertension and diabetes, and decreased endothelial dysfunction created by diabetes and hypertension. PMID: 28296232
    3. Potent natural soluble epoxide hydrolase inhibitors from Pentadiplandra brazzeana baillon: synthesis, quantification, and measurement of biological activities in vitro and in vivo. PMID: 25659109
    4. The results suggest Ephx2 participation in the control of the vascular tone changes in kidney promoting the hypertensive state in the ISIAH rats. PMID: 25509856
    5. Soluble epoxide hydrolase probably plays an important role in the development of hypertension in the rat models of renovascular hypertension. The activation of PPAR-gamma and RAAS by renal arterial stenosis are associated with sEH upregulation. PMID: 22178827
    6. Inhibition of soluble epoxide hydrolase slows the progression of hyperglycemia and provides cardioprotection. PMID: 22865388
    7. Programmed blood pressure-lowering effects of perinatal SEH inhibition cannot be only explained by persistent reduction in renal SEH activity but rather by more complex interactions between the renal SEH, lipoxygenase, and cyclooxygenase pathways. PMID: 21266668
    8. Epoxide hydrolase inhibitors displays antihypertensive effects in Ren-2 transgenic rats with inducible malignant hypertension via an improvement of renal function. PMID: 21078594
    9. Redox regulation of soluble epoxide hydrolase by 15-deoxy-delta-prostaglandin J2 controls coronary hypoxic vasodilation. PMID: 21164107
    10. Soluble epoxide hydrolase enzyme (SEH) inhibition or Ephx2 gene deletion antagonizes neointimal formation in vivo by mechanisms that are endothelium dependent. SEH inhibition may have therapeutic potential for flow-induced remodeling. PMID: 20035028
    11. Epoxide hydrolase plays a role in the regulation of blood pressure PMID: 11882632
    12. Polymorphism in Ephx2 is not likely to contribute to blood pressure levels in spontaneously hypertensive rats. PMID: 12364351
    13. AP-1 activation is involved in the transcriptional up-regulation of sEH by angiotensin II (Ang II) in endothelial cells, which may contribute to Ang II-induced hypertension PMID: 17495027
    14. Linkage analyses with genome-wide expression profiling identified Ephx2 as a heart failure susceptibility gene in SHHF rats. PMID: 18443590
    15. protein level of sEH was elevated in the heart of both spontaneously hypertensive rats and Ang II-infused Wistar rats PMID: 19126686

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  • 亚细胞定位：
    Cytoplasm. Peroxisome.
  • 蛋白家族：
    AB hydrolase superfamily, Epoxide hydrolase family
  • 数据库链接：

    KEGG: rno:65030

    STRING: 10116.ENSRNOP00000023385

    UniGene: Rn.54495