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  4. Recombinant Rat Endothelin-1 (Edn1)

Recombinant Rat Endothelin-1 (Edn1)

  • 货号：
    CSB-YP007400RA
  • 规格：
    ￥2616
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
  • Uniprot No.：
  • 别名：
    Edn1; Endothelin-1; ET-1; Preproendothelin-1; PPET1) [Cleaved into: Big endothelin-1]
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    Full?Length
  • 来源：
    Yeast
  • 分子量：
    29.1 kDa
  • 表达区域：
    53-73aa
  • 氨基酸序列
    CSCSSLMDKECVYFCHLDIIW
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    C-terminal hFc-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    25-35 business days
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Endothelins are endothelium-derived vasoconstrictor peptides. Probable ligand for G-protein coupled receptors EDNRA and EDNRB which activates PTK2B, BCAR1, BCAR3 and, GTPases RAP1 and RHOA cascade in glomerular mesangial cells.
  • 基因功能参考文献：
    1. Results suggest that increases in central ET-1 activity could possibly play a role in chronic estradiol-17beta-induced increase in blood pressure. PMID: 28273940
    2. liraglutide suppresses ET-1 and enhances eNOS/sGC/PKG pathways in treatment of monocrotaline-induced pulmonary arterial hypertension PMID: 27581840
    3. In heart irradiation groups, 3-fold up-regulation of both ET-1 and COX-2 was observed. In pelvis irradiation groups, 3-fold up-regulation of ET-1 was seen, but not significant changes in COX-2 gene expression have observed at distant heart tissues after pelvis irradiation. PMID: 28508833
    4. the mRNA expression of prepro-endothelin-1 (ppET-1), as well as that of the ETA and ETB receptors, were quantified by real-time PCR in these veins... exercise training mobilizes endothelin-1 (ET-1) to reinforce the Ang II-induced responses mainly through ETA activation PMID: 28012855
    5. tonus of the remodeled spiral artery determined by the endovascular trophoblast response to the ET1 and NO systems PMID: 27697218
    6. EDN-1 overexpressed bone marrow-derived mesenchymal stem cells showed increased proliferation and significantly increased osteogenesis potential ability than vector transfected control PMID: 28432001
    7. Activation of renal medullary P2Y2/P2Y4 receptors promotes ET-1-dependent natriuresis in ovariectomized rats. PMID: 28468962
    8. The results of this study indicated that ET1 signaling is critical in maintaining the excitability of glutamatergic neurons in the hippocampus and, thus, in modulating anxiety-like behaviors. PMID: 28302570
    9. the increased contractile response via ET-1 receptors in the ophthalmic artery after 48 hours may elicit negative retinal consequences due to a second ischemic period. PMID: 27322388
    10. ET-1-ROCK interactions contribute to decreased alveolar and vascular growth. PMID: 27760762
    11. Nur77 decreases ET-1 expression by suppressing NF-kappaB and p38 MAPK. PMID: 27765761
    12. Fermented red ginseng ameliorated endothelial dysfunction by downregulation of endothelin-1 (ET-1) and adhesion molecules in the aorta. PMID: 27322312
    13. ET system contributes to pathological neovascularization in diabetes as evidenced by restoration of functional angiogenesis by bosentan treatment and prevention of linagliptin-mediated improvement of angiogenesis in the in vitro model PMID: 26631506
    14. Linagliptin lowered plasma ET-1 levels in diabetes, and reduced ET-1-induced vascular contraction. TLR2 antagonism in diabetic basilar arteries reduced ET-1-mediated cerebrovascular dysfunction and improved endothelium-dependent vasorelaxation PMID: 26898123
    15. Lipopolysaccharides significantly promoted ET-1-induced pulmonary arterial smooth muscle cells proliferation. PMID: 27470334
    16. ET-1-mediated elevation of intracellular Ca(2+) is strongly linked to renal microvascular contraction and is crucial for ET-1-induced contraction of SMC PMID: 26682937
    17. ET-1 may be one of the upstream effectors for programmed neuronal necrosis through abnormal LIMK2 over-expression by ROCK1. PMID: 26438559
    18. Our data demonstrates that endothelin-1-mediated influx of extracellular Ca(2+) activates transient receptor potential canonical channels 1 and 6 in cerebral vascular smooth muscle cells. PMID: 25939574
    19. cerebral microvasculature is relatively insensitive to ET-1-induced functional down-regulation PMID: 25014910
    20. Study showed that store-operated ion channels Orai1 and TRPC3 contribute to endothelin/bradykinin effects in vitro and in vivo PMID: 25873305
    21. hypoxia and anoxia via activation of endothelin-1 at the critical window of heart development inhibits cardiomyocyte proliferation and decreases myocyte endowment in the developing heart PMID: 25692855
    22. in the myocardium of old rats the gene expression of endothelin-1, iNOS and TNF-alpha was increased, and the gene expression of endothelin B receptors and eNOS was reduced, compared with young rats PMID: 25446983
    23. ET-1 and ETAR are potential targets responsible for the observed synergism effect in the hypertensive atherosclerotic process through enhancement of ET-1 binding, ET-1 binding, ETAR expression, and ET-1-induced mitogenic actions in aortic VSMCs PMID: 25824048
    24. ET-1 stimulates ETA -mediated NADPH oxidase-dependent ROS generation, which inhibits endothelial NO bioavailability and contributes to ET-1-induced contraction in healthy penile arteries. PMID: 25091502
    25. ROCK-2 inhibition restores ET-1-mediated NO production after the LPS pretreatment, in part, through an increase in actin depolymerization PMID: 25243430
    26. Salt or water loading increased ET-1 mRNA in inner medullary collecting duct PMID: 25587122
    27. Data show an inappropriate secretion of endothelin 1 (ET-1) in obese animals with a parallel DNA damage in their lungs. PMID: 25517973
    28. High glucose induced ET-1 production that mediated the EMT induced by high glucose in renal tubular epithelial cells, and HIF-1alpha acted as the upstream signal to regulate ET-1. PMID: 24447812
    29. We conclude that enhanced ET-1 signaling is part of a pathologic mechanism associated with adverse cerebrovascular outcomes of obstructive sleep apnea ( PMID: 25425077
    30. Restriction in cerebral blood flow was observed with upregulation of endothelin-1 in cerebral ischemia. PMID: 25228257
    31. These findings identify a novel mechanistic interaction between the ET-1/ETB receptor axis and CX3CL1/CX3CR1 in mediating pulmonary angiogenesis and vascular monocyte accumulation in experimental HPS. PMID: 24731444
    32. The combination therapy prevented pulmonary artery injury and angiogenesis of the arteries by reducing the level of ET-1 and promoting the level of 6-keto-PGF1alpha in the blood PMID: 24854661
    33. Endothelin-1 and endothelin-2 initiate and maintain contractile responses by different mechanisms in rat mesenteric and cerebral arteries. PMID: 23941276
    34. Ang II enhances ET-1-induced vasoconstriction by upregulating ETAR expression and ET-1/ETAR binding, which may be because of the AngII/Ang II receptor pathways and the activation of PKC or ERK. PMID: 25088996
    35. Altered pulmonary vascular reactivity in hypertension may be related to a loss of endothelial buffering of vasoconstriction and decreased leptin-induced vasodilation in conditions of increased endothelin-1. PMID: 24499246
    36. The insulin/endothelin-1 vasoconstrictor pathway is more active in GFA than in SFA. PMID: 23995100
    37. These results indicated that apocynin alleviated CIH-induced hypertension by inhibiting NADPH oxidase, further leading to the reduced vasoconstrictor ET-1 level and oxidative stress. PMID: 23592126
    38. COX-2 and NFATc3 signaling are involved in ET-1-induced hypertrophy of neonatal rat cardiomyocytes. PMID: 24291639
    39. These findings support the hypothesis that vascular endoplasmic reticulum stress-mediated activation of ET-1 may be an underlying cause of impaired vasomotor responsiveness to insulin and endothelial dysfunction. PMID: 24304569
    40. ET-1 induces a glycolytic switch in pulmonary arterial endothelial cells via the redistribution of uncoupled eNOS. PMID: 24392990
    41. Endothelin-1 has a role in activating ERK1/2 via transactivation of platelet-derived growth factor receptor in rat L6 myoblasts PMID: 24735959
    42. Levels of ET-1 peptide increase in kidney tissues after lipopolysaccharide (LPS) administration time-dependently with a rise in plasma ET-1 concentration, compared to control rats. PMID: 24641950
    43. ET-1-induced cardiomyocyte hypertrophy is mediated through peptidylprolyl cis/trans isomerase (Pin1) activation. PMID: 24657892
    44. Status epilepticus induces vasogenic edema via TNF-alpha/endothelin-1-mediated two different pathways. PMID: 24040253
    45. CIH induced increased ET-1. PMID: 23662699
    46. NO, PGI2 and ET-1 expression are regulated by 17beta-estradiol in pulmonary hypertension PMID: 22936708
    47. ET-1 enhances the phosphorylation of epidermal growth factor receptor (EGFR) in cultured vascular smooth muscle cells. PMID: 23537435
    48. The increased responsiveness to ET-1 induced by intermittent hypoxia in pulmonary arteries of rats was due to increased expression of ETA receptors PMID: 23555567
    49. Epididymal fat and soleus muscles from rat were incubated with ADM at concentration of 100 nM for the study of the gene expression and secretion of tumour necrosis factor (TNF-alpha), EDN-1, leptin, adiponectin, interleukin 1beta (IL-1beta), and IL-6. PMID: 22956308
    50. In conclusion, the cardioprotective effects of EGb761 on markers of ADR-induced acute cardiotoxicity appeared to have been mediated by the regulation of inflammatory and vasoactive mediators, as well as the inhibition of membrane lipid peroxidation. PMID: 23898483

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  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Endothelin/sarafotoxin family
  • 数据库链接：

    KEGG: rno:24323

    STRING: 10116.ENSRNOP00000019361

    UniGene: Rn.10918