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  4. Recombinant Rat Endophilin-A1 (Sh3gl2)

Recombinant Rat Endophilin-A1 (Sh3gl2)

  • 货号：
    CSB-YP021231RA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP021231RA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP021231RA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP021231RA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP021231RA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Sh3gl2; Sh3d2a; Sh3p4; Endophilin-A1; Endophilin-1; SH3 domain protein 2A; SH3 domain-containing GRB2-like protein 2; SH3p4
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-352
  • 氨基酸序列
    MSVAGLKKQF HKATQKVSEK VGGAEGTKLD DDFKEMERKV DVTSRAVMEI MTKTIEYLQP NPASRAKLSM INTMSKIRGQ EKGPGYPQAE ALLAEAMLKF GRELGDDCNF GPALGEVGEA MRELSEVKDS LDMEVKQNFI DPLQNLHDKD LREIQHHLKK LEGRRLDFDY KKKRQGKIPD EELRQALEKF DESKEIAESS MFNLLEMDIE QVSQLSALVQ AQLEYHKQAV QILQQVTVRL EERIRQASSQ PRREYQPKPR MSLEFATGDG TQPNGGLSHT GTPKPAGVQM DQPCCRALYD FEPENEGELG FKEGDIITLT NQIDENWYEG MLHGQSGFFP INYVEILVAL PH
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Implicated in synaptic vesicle endocytosis. May recruit other proteins to membranes with high curvature. Required for BDNF-dependent dendrite outgrowth. Cooperates with SH3GL2 to mediate BDNF-NTRK2 early endocytic trafficking and signaling from early endosomes.
  • 基因功能参考文献：
    1. Together, these observations suggest that while endophilin helps shape endocytic tubules and recruit dynamin to endocytic sites, it can also block membrane fission when present in excess by inhibiting inter-dynamin interactions. PMID: 28933693
    2. These data suggested a role of EndoII in protecting BASMCs apoptosis induced by H2O2, possibly by inhibiting the addressing of Bax to mitochondria. Targeting on EndoII may be a new strategy to treat apoptosis-associated diseases. PMID: 26854628
    3. We find that the phosphomimetic mutation S75D favors vesicle formation by inhibiting this conformational switch, acting to regulate endophilin A1-mediated curvature. PMID: 24778241
    4. a coarse-grained model was used to study various membrane remodeling scenarios induced by endophilin N-terminal helix and Bin/amphiphysin/Rvs domains. PMID: 23442862
    5. Kinetics of endophilin N-BAR domain dimerization and membrane interactions. PMID: 23482561
    6. Endophilin I and endophilin II interact with N-type Ca2+ channel carboxyl-terminal fragments in clathrin-mediated endocytosis. PMID: 22961472
    7. measurements of curvature sorting and curvature generation of the endophilin A1 N-BAR domain on tubular membranes pulled from giant unilamellar vesicles PMID: 22768939
    8. Study determined the structure of residues 63-86, a region that is disordered in the crystal structure of endophilin A1. PMID: 20418375
    9. the adaptability of endophilin-lipid interactions underlies dynamic changes of endocytic membranes PMID: 20484046
    10. VGLUT1 and endophilin 1 and 3 may interact to play an important role affecting the vesicular sequestration and synaptic release of glutamate. PMID: 16710756
    11. Crystal structure of endophilin A2 adheres to the canonical SH3-domain fold and is highly similar to those of the corresponding domains of endophilins A1 and A3. PMID: 18391417

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  • 亚细胞定位：
    Cytoplasm. Membrane; Peripheral membrane protein. Early endosome. Cell junction, synapse, presynapse.
  • 蛋白家族：
    Endophilin family
  • 组织特异性：
    Expressed in the brain. Expressed at low level in the kidney.
  • 数据库链接：

    KEGG: rno:116743

    STRING: 10116.ENSRNOP00000008999

    UniGene: Rn.10787