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  4. Recombinant Rat Egl nine homolog 1 (Egln1)

Recombinant Rat Egl nine homolog 1 (Egln1)

  • 货号：
    CSB-YP007481RA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP007481RA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP007481RA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP007481RA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP007481RA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Egln1Egl nine homolog 1; EC 1.14.11.29; Hypoxia-inducible factor prolyl hydroxylase 2; HIF-PH2; HIF-prolyl hydroxylase 2; HPH-2; Prolyl hydroxylase domain-containing protein 2; PHD2; Fragment
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-222
  • 氨基酸序列
    MNKHGICVVD DFLGRETGQQ IGDEVRALHD TGKFTDGQLV SQKSDSSKDI RGDKITWIEG KEPGCETIGL LMSSMDDLIR HCSGKLGNYR INGRTKAMVA CYPGNGTGYV RHVDNPNGDG RCVTCIYYLN KDWDAKVSGG ILRIFPEGKA QFADIEPKFD RLLFFWSDRR NPHEVQPAYA TRYAITVWYF DADERARAKV KYLTGEKGVR VELKPNSVSK DV
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Cellular oxygen sensor that catalyzes, under normoxic conditions, the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates a specific proline found in each of the oxygen-dependent degradation (ODD) domains (N-terminal, NODD, and C-terminal, CODD) of HIF1A. Also hydroxylates HIF2A. Has a preference for the CODD site for both HIF1A and HIF1B. Hydroxylated HIFs are then targeted for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. Under hypoxic conditions, the hydroxylation reaction is attenuated allowing HIFs to escape degradation resulting in their translocation to the nucleus, heterodimerization with HIF1B, and increased expression of hypoxy-inducible genes. EGLN1 is the most important isozyme under normoxia and, through regulating the stability of HIF1, involved in various hypoxia-influenced processes such as angiogenesis in retinal and cardiac functionality. Target proteins are preferentially recognized via a LXXLAP motif.
  • 基因功能参考文献：
    1. PHD2 controls TNF-alpha effects by positively regulating NF-kappaB signaling, whereas NF-kappaB mediates cytokine-dependent PHD2 expression. PMID: 25635047
    2. PHD-2 KO mice showed significantly accelerated wound closure relative to WT. PMID: 24695462
    3. Impaired PHD2 response to high salt intake in the renal medulla may represent a novel mechanism for hypertension in Dahl S rats, and inhibition of PHD2 in the renal medulla could be a therapeutic approach for salt-sensitive hypertension. PMID: 24190904
    4. Renal medullary PHD2 is an important regulator in renal adaptation to high salt intake. PMID: 22686466
    5. PHD2 may directly interact with PDE4D to function as a novel regulator of the intracellular cAMP levels in cardiomyocytes. PMID: 22975349
    6. Data show that knockdown of PHD2, a major isoform of PHDs, by RNA interference reduced AT(1)R expression. PMID: 21825224
    7. High-salt intake inhibits PHD2 in the renal medulla, thereby upregulating the HIF-1alpha expression in rat renal medulla. PMID: 20308610
    8. identified the three forms of prolyl hydroxylase in rats and demonstrated that PHD2 has a role in proline hydroxylation, which regulates hypoxic preconditioning PMID: 12876291
    9. Decrease in PHD2 expression level is associated with ischemia. PMID: 19349364

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  • 亚细胞定位：
    Cytoplasm. Nucleus.
  • 组织特异性：
    Expressed in heart, liver, kidney, brain, liver and testis. Highest levels in heart, lowest in liver.
  • 数据库链接：

    KEGG: rno:308913

    STRING: 10116.ENSRNOP00000026767

    UniGene: Rn.232203