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  4. Recombinant Rat E3 ubiquitin-protein ligase UHRF1 (Uhrf1)

Recombinant Rat E3 ubiquitin-protein ligase UHRF1 (Uhrf1)

  • 中文名称：
    大鼠Uhrf1重组蛋白
  • 货号：
    CSB-YP759727RA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    大鼠Uhrf1重组蛋白
  • 货号：
    CSB-EP759727RA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    大鼠Uhrf1重组蛋白
  • 货号：
    CSB-EP759727RA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    大鼠Uhrf1重组蛋白
  • 货号：
    CSB-BP759727RA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    大鼠Uhrf1重组蛋白
  • 货号：
    CSB-MP759727RA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Uhrf1
  • Uniprot No.：
  • 别名：
    Uhrf1; Ac2-121; E3 ubiquitin-protein ligase UHRF1; EC 2.3.2.27; Liver regeneration-related protein LRRG126; RING-type E3 ubiquitin transferase UHRF1; Ubiquitin-like PHD and RING finger domain-containing protein 1; Ubiquitin-like-containing PHD and RING finger domains protein 1
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-774
  • 氨基酸序列
    MWIQVRTMDG KETHTVNSLS RLTKVQELRK KIEELFHVEP QLQRLFYRGK QMEDGHTLFD YDVRLNDTIQ LLVRQSLALP LSTKERDSEL SDSDSGYGVG HSESDKSSTH GEGTADGDDK TVWEDTDLGL YKVNEYVDVR DNIFGAWFEA QVVQVQKKAL SEEEPCSSSA IMAPEDDIMY HIKYDDYPEH GVDIVKAKNV RARARTVIPW EDLEVGQVVM ANYNVDYPRK RGFWYDVEIC RKRQTRTARE LYGNVMLLND SQLNNCRIIF VDEVLKIELP NERSPLIGSP SRRKSGPSCQ YCKDDENKPC RKCACHICGG REAPEKQVLC DECDMAFHLY CLQPPLTCVP PEPEWYCPSC RTDSSEVVQA GEKLKKSKKK AKMASATSSS RRDWGKGMAC VGRTTECTIV PANHFGPIPG VPVGTMWRFR VQVSESGVHR PHVAGIHGRS NDGAYSLVLA GGYEDDVDNG NFFTYTGSGG RDLSGNKRTA GQSSDQKLTN NNRALALNCH SPINEKGAEA EDWRQGKPVR VVRNMKGGKH SKYAPAEGNR YDGIYKVVKY WPEKGKSGFI VWRYLLRRDD TEPEPWTREG KDRTRQLGLT MQYPEGYLEA LANKEKNRKR PAKALEQGPS SSKIGKSKRK STGPATTSPR VSKKSKLEPY TLPLQQANLI KEDKGNAKLW DDVLSSLQDG PYQIFLSKVK EAFQCICCQE LVFRPVTTVC QHNVCKDCLD RSFRAQVFSC PACRYDLDHS SPTRVNQPLQ TILNQLFPGY GSGR
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Multidomain protein that acts as a key epigenetic regulator by bridging DNA methylation and chromatin modification. Specifically recognizes and binds hemimethylated DNA at replication forks via its YDG domain and recruits DNMT1 methyltransferase to ensure faithful propagation of the DNA methylation patterns through DNA replication. In addition to its role in maintenance of DNA methylation, also plays a key role in chromatin modification: through its tudor-like regions and PHD-type zinc fingers, specifically recognizes and binds histone H3 trimethylated at 'Lys-9' (H3K9me3) and unmethylated at 'Arg-2' (H3R2me0), respectively, and recruits chromatin proteins. Enriched in pericentric heterochromatin where it recruits different chromatin modifiers required for this chromatin replication. Also localizes to euchromatic regions where it negatively regulates transcription possibly by impacting DNA methylation and histone modifications. Has E3 ubiquitin-protein ligase activity by mediating the ubiquitination of target proteins such as histone H3 and PML. It is still unclear how E3 ubiquitin-protein ligase activity is related to its role in chromatin in vivo. Plays a role in DNA repair by cooperating with UHRF1 to ensure recruitment of FANCD2 to interstrand crosslinks (ICLs) leading to FANCD2 activation.
  • 基因功能参考文献：
    1. UHRF1 inhibits the late phase of oligodendrocyte differentiation. PMID: 17065439
  • 亚细胞定位：
    Nucleus.
  • 数据库链接：

    KEGG: rno:316129

    STRING: 10116.ENSRNOP00000066794

    UniGene: Rn.54318