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  4. Recombinant Rat Dynamin-1-like protein (Dnm1l)

Recombinant Rat Dynamin-1-like protein (Dnm1l)

  • 货号：
    CSB-YP007078RA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP007078RA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP007078RA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP007078RA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP007078RA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Dnm1l; Dlp1; Drp1Dynamin-1-like protein; EC 3.6.5.5; Dynamin-like protein
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-755
  • 氨基酸序列
    MEALIPVINK LQDVFNTVGA DIIQLPQIVV VGTQSSGKSS VLESLVGRDL LPRGTGVVTR RPLILQLVHV SPEDKRKTTG EENDPATWKN SRHLSKGVEA EEWGKFLHTK NKLYTDFDEI RQEIENETER ISGNNKGVSP EPIHLKVFSP NVVNLTLVDL PGMTKVPVGD QPKDIELQIR ELILRFISNP NSIILAVTAA NTDMATSEAL KISREVDPDG RRTLAVITKL DLMDAGTDAM DVLMGRVIPV KLGIIGVVNR SQLDINNKKS VTDSIRDEYA FLQKKYPSLA NRNGTKYLAR TLNRLLMHHI RDCLPELKTR INVLAAQYQS LLNSYGEPVD DKSATLLQLI TKFATEYCNT IEGTAKYIET SELCGGARIC YIFHETFGRT LESVDPLGGL NTIDILTAIR NATGPRPALF VPEVSFELLV KRQIKRLEEP SLRCVELVHE EMQRIIQHCS NYSTQELLRF PKLHDAIVEV VTCLLRKRLP VTNEMVHNLV AIELAYINTK HPDFADACGL MNNNIEEQRR NRLARELPSA VSRDKSSKVP SALAPASQEP SPAASAEADG KLIQDNRRET KNVASAGGGI GDGGRIGDGG QEPTTGNWRG MLKTSKAEEL LAEEKSKPIP IMPASPQKGH AVNLLDVPVP VARKLSAREQ RDCEVIERLI KSYFLIVRKN IQDSVPKAVM HFLVNHVKDT LQSELVGQLY KSSLLDDLLT ESEDMAQRRK EAADMLKALQ GASQIIAEIR ETHLW
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Functions in mitochondrial and peroxisomal division. Mediates membrane fission through oligomerization into membrane-associated tubular structures that wrap around the scission site to constrict and sever the mitochondrial membrane through a GTP hydrolysis-dependent mechanism. The specific recruitment at scission sites is mediated by membrane receptors like MFF, MIEF1 and MIEF2 for mitochondrial membranes. While the recruitment by the membrane receptors is GTP-dependent, the following hydrolysis of GTP induces the dissociation from the receptors and allows DNM1L filaments to curl into closed rings that are probably sufficient to sever a double membrane. Acts downstream of PINK1 to promote mitochondrial fission in a PRKN-dependent manner. Plays an important role in mitochondrial fission during mitosis. Through its function in mitochondrial division, ensures the survival of at least some types of postmitotic neurons, including Purkinje cells, by suppressing oxidative damage. Required for normal brain development, including that of cerebellum. Facilitates developmentally regulated apoptosis during neural tube formation. Required for a normal rate of cytochrome c release and caspase activation during apoptosis; this requirement may depend upon the cell type and the physiological apoptotic cues. Required for formation of endocytic vesicles. Proposed to regulate synaptic vesicle membrane dynamics through association with BCL2L1 isoform Bcl-X(L) which stimulates its GTPase activity in synaptic vesicles; the function may require its recruitment by MFF to clathrin-containing vesicles. Required for programmed necrosis execution. Rhythmic control of its activity following phosphorylation at Ser-656 is essential for the circadian control of mitochondrial ATP production.
  • 基因功能参考文献：
    1. knockdown of LRP6 inhibited the cell viability by activation of Drp1 in glucose deprived-cardiomyocytes. PMID: 29864925
    2. In aortas of CKD rats and hippurate-treated rats, we observed an increase in Drp1 protein levels and mitochondrial fission. Inhibition of Drp1 improved endothelial function in both rat models. These results indicate that hippurate, by itself, can cause endothelial dysfunction. Increased mitochondrial fission plays an active role in hippurate-induced endothelial dysfunction via an increase in mitoROS PMID: 29573704
    3. RhoA/ROCK pathways are involved in the phosphorylation and mitochondrial translocation of Drp1. PMID: 29336470
    4. s demonstrate that YQFM ameliorates ischemic stroke-induced neuronal apoptosis through inhibiting mitochondrial dysfunction and PKCdelta/Drp1-mediated excessive mitochondrial fission. PMID: 29435096
    5. miR-21-5p/203a-3p promote ox-LDL-induced endothelial senescence through down-regulation of Drp1 in a direct or indirect way. PMID: 28347692
    6. Right ventricular ischemia occurs in pulmonary arterial hypertension and causes Drp1-Fis1-mediated fission leading to diastolic dysfunction. PMID: 28265681
    7. Drp1-dependent mitochondrial fission changes in the dorsal vagal complex regulate insulin action. PMID: 28273447
    8. Report a novel non-canonical function of the fission protein, DRP1 in maintaining or positively stimulating mitochondrial respiration, bioenergetics and ROS signalling in adult cardiomyocyte, which is likely independent of morphological changes. PMID: 27794519
    9. The hypoxia-induced DRP1 expression depends on ROS generation, especially mitochondrial ROS (mROS). Moreover, the levels of ROS and mROS evoked by hypoxia were regulated by DRP1. PMID: 27010815
    10. Drp-1 inhibition blocked BPA-mediated Drp-1 translocation, leading to decreased apoptosis of Neural Stem Cell. PMID: 27252377
    11. Neurons overexpressing wild-type Drp1 promoted mitochondrial and nuclear fragmentation; however, neurons overexpressing dominant-negative Drp1(K38A) or cotreated with melatonin exhibited significantly reduced MPP(+) -induced mitochondrial fragmentation and neuron death. PMID: 27159033
    12. Melatonin's protective effects were attributed to its roles in preventing cytosolic calcium ([Ca(2+) ]i ) overload, which blocked the recruitment of Drp1 from the cytoplasm to the mitochondria. PMID: 26732476
    13. Inhibition of Drp1 prevents mitochondrial fission and reduces mechanical allodynia induced by perineural HIV-1 gp120. PMID: 26418124
    14. Results show that Drp1 regulates proliferation and migration of vascular smooth muscle cells as well as neointimal formation in an carotid artery balloon injury model. . PMID: 25399916
    15. NAC was able to prevent the rotenone-induced changes in parkin and Drp1 levels in the both studied areas. NAC delayed the Parkinson's disease induction by rotenone and this effect might be related to its proved antioxidant effect. PMID: 25732239
    16. findings indicated that PINK1 is an endogenous protective mediator vital for neuronal survival under ischemic insult through regulating Drp1 phosphorylation at Ser616 PMID: 25791474
    17. Results showed that under hypoxic/ischemic stress a Drp1-dependent mitophagy was triggered which was involved in the removal of damaged mitochondria and cellular survival at the early stage of hypoxic/ischemic injury PMID: 25018043
    18. Phosphorylation of Drp1 at serine 616 (S616) is mediated by cyclin-dependent kinase 5 (CDK5) in post-mitotic rat neurons. PMID: 25012575
    19. Drp1 inhibition triggers cardioprotection by reducing mitochondrial metabolism during ischemia reperfusion injury. PMID: 24477044
    20. Opa-1 and Drp-1 in manganese-induced apoptosis PMID: 24632637
    21. nitrite-induced increased mitochondrial superoxide generation dependent on the phosphorylation of Drp1 PMID: 24081164
    22. Phosphorylation of DRP1 S622 was confirmed in phenylephrine-treated neonatal cardiomyocytes in an in vitro model of pressure-overloaded cardiac hypertrophy . PMID: 23882026
    23. PINK1 ameliorated ischemia induced cell death and energy disturbance including reduced ATP generation and collapse of mitochondrial membrane potential by attenuating mitochondrial function. PMID: 23772688
    24. Constitutive Drp1 overexpression leads to a state of chronic, abortive mitochondrial hyperfission. PMID: 23764851
    25. Altogether, our data suggested that mdivi-1 exerts neuroprotective effects against cell death of hippocampal neurons induced by seizures, and the underlying mechanism may be through inhibiting CytC release. PMID: 23628672
    26. Pim-1 activity prevents Drp1 compartmentalization to the mitochondria and preserves reticular mitochondrial morphology in response to sI PMID: 23530233
    27. Drp1 LXVP motif shapes mitochondria in neuronal and non-neuronal cells, and that CaN-mediated Drp1 dephosphorylation promotes neuronal death PMID: 23486469
    28. Over-expression of DRP-1 led to aggravated INS-1-derived pancreatic beta cell apoptosis triggered by free fatty acids. PMID: 23166623
    29. Gliclazide showed protective effect on DPN through modulating Drp-1-mediated oxidative stress and apoptosis. PMID: 22732450
    30. the involvement of DRP1 in physiological regulation of brain glucose-induced insulin secretion and food intake inhibition PMID: 22229526
    31. a model for regulation of Drp1-dependent mitochondrial fission PMID: 22334657
    32. DRP-1-mediated mitotic fission is a cell-cycle checkpoint that can be therapeutically targeted in hyperproliferative disorders such as pulmonary arterial hypertension. PMID: 22511751
    33. These studies provide support for a substantial role of mitochondrial fission in preclinical models of inflammatory and neuropathic pain. PMID: 21813700
    34. dynamin-related protein 1 (DRP-1), plays an important role in ER stress-induced beta-cell apoptosis PMID: 21537829
    35. phosphorylation of Drp1 on Ser-585 promotes mitochondrial fission in mitotic cells[Drp1] PMID: 17301055
    36. Data indicate a role for DLP1 as a novel component of the apical sorting machinery at the trans-Golgi network. PMID: 20688057
    37. The Drp1 is required for the release of endonuclease G from mitochondria. PMID: 20594982
    38. MiR-30 family members inhibit mitochondrial fission through targeting p53 and the dynamin-related protein-1 pathway. PMID: 20062521
    39. DLP1 also caused global morphological changes in mitochondrial outer membrane-like liposomes, but DLP1 did not stimulate BAX-permeabilizing function in the absence or presence of Bif-1. PMID: 19074440

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  • 亚细胞定位：
    Cytoplasm, cytosol. Golgi apparatus. Endomembrane system; Peripheral membrane protein. Mitochondrion outer membrane; Peripheral membrane protein. Peroxisome. Membrane, clathrin-coated pit. Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane.
  • 蛋白家族：
    TRAFAC class dynamin-like GTPase superfamily, Dynamin/Fzo/YdjA family
  • 组织特异性：
    Expressed in all tissues tested (at protein level). Longer isoforms are preferentially expressed in brain.
  • 数据库链接：

    KEGG: rno:114114

    STRING: 10116.ENSRNOP00000002478

    UniGene: Rn.216851