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  4. Recombinant Rat DnaJ homolog subfamily C member 5 (Dnajc5)

Recombinant Rat DnaJ homolog subfamily C member 5 (Dnajc5)

  • 货号：
    CSB-YP007037RA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP007037RA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP007037RA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP007037RA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP007037RA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Dnajc5DnaJ homolog subfamily C member 5; Cysteine string protein; CSP
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-198
  • 氨基酸序列
    MADQRQRSLS TSGESLYHVL GLDKNATSDD IKKSYRKLAL KYHPDKNPDN PEAADKFKEI NNAHAILTDA TKRNIYDKYG SLGLYVAEQF GEENVNTYFV LSSWWAKALF VVCGLLTCCY CCCCLCCCFN CCCGKCKPKA PEGEETEFYV SPEDLEAQLQ SDEREATDTP IVIQPASATE TTQLTADSHP SYHTDGFN
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Acts as a general chaperone in regulated exocytosis. Acts as a co-chaperone for the SNARE protein SNAP-25. Involved in the calcium-mediated control of a late stage of exocytosis. May have an important role in presynaptic function. May be involved in calcium-dependent neurotransmitter release at nerve endings.
  • 基因功能参考文献：
    1. CSPalpha may modulate fusion pore dynamics in a phosphorylation-dependent manner. PMID: 24956274
    2. both the J-domain and the linker domain of CSP1 are necessary to function in an important role in acinar cell exocytosis PMID: 23942053
    3. phosphorylation-dependent interaction with synaptotagmin I PMID: 11931641
    4. The presence of cysteine-string protein in synaptic vesicles of inner hair cells and its synchronized expression with the appearance of cochlear potentials suggests that this protein mediates sound-evoked glutamate release by inner hair cells. PMID: 12028351
    5. A novel alphaGDI complex from synaptic membranes contains three chaperone components: Hsp90, Hsc70 and cysteine string protein (CSP). The complex is dissociated in response to Ca(2+)-induced neurotransmitter release. PMID: 12426384
    6. Data suggest that cysteine string protein self-interactions may be an important consideration for current working models of CSP chaperone activity at the synapse. PMID: 12559961
    7. distinct binding sites of CSP can play a role in modulating G protein function and G protein inhibition of calcium channels PMID: 12783986
    8. cysteine string protein binds to a brain-specific isoform of small glutamine-rich tetratricopeptide repeat-containing protein PMID: 12878599
    9. Cysteine string protein inhibits N-type calcium channels, but is blocked by mutant huntingtin PMID: 14570907
    10. Proportion of the phosphorylated protein varies between distinct brain regions and also exhibits developmental regulation. PMID: 15926915
    11. CSPalpha activity is subject to regulation by factors that alter Hsc70 distribution and translocation within the cell PMID: 17113038
    12. palmitoylation of CSP is enhanced specifically by co-expression of the Golgi-localized palmitoyl transferases DHHC3, DHHC7, DHHC15, or DHHC17 PMID: 18596047
    13. Establish a role for CSP-alpha in regulating digestive enzyme secretion and suggest that CSP-alpha and Hsc70 modulate specific soluble N-ethylmaleimide-sensitive attachment receptor interactions necessary for exocytosis. PMID: 19282376

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  • 亚细胞定位：
    Cytoplasm, cytosol. Membrane; Lipid-anchor. Cytoplasmic vesicle, secretory vesicle, chromaffin granule membrane. Melanosome. Cell membrane.
  • 组织特异性：
    Brain. Predominantly associated with nerve endings and synaptic vesicles.
  • 数据库链接：

    KEGG: rno:79130

    STRING: 10116.ENSRNOP00000020581

    UniGene: Rn.100120