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  4. Recombinant Rat DNA-binding protein inhibitor ID-1 (Id1)

Recombinant Rat DNA-binding protein inhibitor ID-1 (Id1)

  • 货号：
    CSB-YP010966RA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP010966RA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP010966RA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP010966RA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP010966RA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Id1; Id-1; DNA-binding protein inhibitor ID-1; Inhibitor of DNA binding 1; Inhibitor of differentiation 1
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-164
  • 氨基酸序列
    MKVASSSAAA TAGPSCSLKA GRTAGEVVLG LSEQSVAISR CAGTRLPALL DEQQVNVLLY DMNGCYSRLK ELVPTLPQNR KVSKVEILQH VIDYIRDLQL ELNSESEVAT AGGRGLPVRA PLSTLNGEIS ALAAEVRSES EYYIILLWET KATGGGCPPY FSGA
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Transcriptional regulator (lacking a basic DNA binding domain) which negatively regulates the basic helix-loop-helix (bHLH) transcription factors by forming heterodimers and inhibiting their DNA binding and transcriptional activity. Implicated in regulating a variety of cellular processes, including cellular growth, senescence, differentiation, apoptosis, angiogenesis, and neoplastic transformation. Inhibits skeletal muscle and cardiac myocyte differentiation. Regulates the circadian clock by repressing the transcriptional activator activity of the CLOCK-ARNTL/BMAL1 heterodimer.
  • 基因功能参考文献：
    1. Via RNAi, it was found that ID1 induction was inhibited with silencing of HIF-1alpha. Moreover, the suppression of ID1 mRNA expression could lead to decreased expression and transcription of HIF-1alpha during hypoxia and reoxygenation. In addition, it was demonstrated that both ID1 and HIF-1alpha can regulate the transcription of twist PMID: 27127787
    2. These results thus established a signaling cascade of "Abeta --> Id1 --> HIF-1 --> SHH" in primary rat cortical cultures; furthermore, SHH may in part contribute to amyloid beta neurotoxicity. PMID: 25502463
    3. Smad1 as a novel binding protein of KSHV latency-associated nuclear antigen (LANA). LANA interacted with and sustained BMP-activated p-Smad1 in the nucleus and enhanced its loading on the Id promoters. PMID: 25010525
    4. These data suggest that BMP4 may regulate survival through the Id1/EGFR pathway during development of the rat inner ear. PMID: 23335245
    5. Results suggest that the Id1/PI3K/Akt/NFkappaB/survivin signalling pathway plays a critical role in endothelial progenitor cell proliferation. PMID: 22139302
    6. Recombinant human bone morphogenetic protein 2 enhanced mesenchymal stem cell Id1 expression, required for vascular differentiation and spatial organisation, under normoxic conditions. PMID: 21472453
    7. Prostacyclin analogues inhibited smooth muscle cell proliferation and prevented progression of pulmonary hypertension while enhancing Smad1/5 phosphorylation and Id1 gene expression. PMID: 20522807
    8. the exclusion of Id-1 from GFAP-positive cells, and expression in a population of vimentin-positive pineal astrocytes is evidence of a role for Id-1 in the adult stabilization of one form of astrocyte PMID: 12495223
    9. Treatment of PC12 cells with recombinant Tat enhances the NGF-induced Id1 expression, further pointing to Id1 as a target for Tat. PMID: 15361847
    10. induced in the presence of Epstein-Barr virus LMP1 and confirmed by mRNA and protein in C33A and Rat-1 cells PMID: 15564458
    11. overexpression of ID1 and ID2 genes in a postmitotic, terminally differentiated cell type (Sertoli cells)have the capacity to induce reentry into the cell cycle PMID: 15647457
    12. ID-1 proteins play an important role in the development of acquired drug resistance to various drugs of prostate cancer cells. (Id-1) PMID: 15809768
    13. Expression of all 4 members of the Id family of transcription factors is differentially regulated in response to orchidectomy along the epididymis. PMID: 16304207
    14. A 31-bp sequence (Box-2 region), located between -200 and -169bp in the Id-1 promoter is necessary for the promoter activity. PMID: 16638616
    15. Drug resistant prostate cancer cells displayed significantly increased expression of ID1 compared with other prostate cancer PMID: 16728343
    16. The proliferative action of Id1 on the organ of Corti is mediated by nuclear factor-kappaB (NF-kappaB) and cyclin D1 in the embryonic cochlea. PMID: 17149750

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  • 亚细胞定位：
    Cytoplasm. Nucleus.
  • 数据库链接：

    KEGG: rno:25261

    STRING: 10116.ENSRNOP00000052261

    UniGene: Rn.2113