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  4. Recombinant Rat Cell division cycle protein 20 homolog (Cdc20)

Recombinant Rat Cell division cycle protein 20 homolog (Cdc20)

  • 货号：
    CSB-YP737086RA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP737086RA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP737086RA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP737086RA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP737086RA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Cdc20Cell division cycle protein 20 homolog; p55CDC
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-499
  • 氨基酸序列
    MAQFVFESDL HSLLQLDAPI PNAPIARWQR KAKEATGPAP SPMRAANRSH SAGRTPGRTP GKSNSKVQTT PSKPGGDRYI PQRSASQMEV ASFLLSKENQ PEDGGTPTKK EHQKAWARNL NGFDVEEAKI LRLSGKPQNA PEGYQNRLKV LYSQKATPGS SRKACRYIPS LPDRILDAPE IRNDYYLNLV DWSSGNVLAV ALDNSVYLWN AGSGDILQLL QMEQPGDYIS SVAWIKEGNY LAVGTSNAEV QLWDVQQQKR LRNMTSHSAR VSSLSWNSYI LSSGSRSGHI HHHDVRVAEH HVATLSGHSQ EVCGLRWAPD GRHLASGGND NIVNVWPSGP GESGWVPLQT FTQHQGAVKA VAWCPWQSNI LATGGGTSDR HIRIWNVCSG ACLSAVDVHS QVCSILWSPH YKELISGHGF AQNQLVIWKY PTMAKVAELK GHTARVLSLT MSPDGATVAS AAADETLRLW RCFELDPALR REREKASTSK SSLIHQGIR
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Required for full ubiquitin ligase activity of the anaphase promoting complex/cyclosome (APC/C) and may confer substrate specificity upon the complex. Is regulated by MAD2L1: in metaphase the MAD2L1-CDC20-APC/C ternary complex is inactive and in anaphase the CDC20-APC/C binary complex is active in degrading substrates. The CDC20-APC/C complex positively regulates the formation of synaptic vesicle clustering at active zone to the presynaptic membrane in postmitotic neurons. CDC20-APC/C-induced degradation of NEUROD2 induces presynaptic differentiation. Interacts with NEK2.
  • 基因功能参考文献：
    1. CDK2 blocks APC/CCdc20-mediated ubiquitylation of p21 at the N terminus, causing G2 arrest in mitogen-stimulated cardiomyocytes PMID: 22045811
    2. The functional roles of Cdc20 protein is involved in the cell cycle, mitosis, and apoptosis in CBRH-7919 cells. PMID: 20517887
    3. The expression level and activities of PC-PLC changed in a cell-cycle-dependent manner and were inversely correlated with the expression of Cdc20 PMID: 19347873
    4. findings define a Cdc20-APC(anaphase promoting complex) ubiquitin signaling pathway that governs presynaptic development, which holds important implications for neuronal connectivity and plasticity in the brain PMID: 19900895
  • 亚细胞定位：
    Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle pole.
  • 蛋白家族：
    WD repeat CDC20/Fizzy family
  • 数据库链接：

    KEGG: rno:64515

    STRING: 10116.ENSRNOP00000037772

    UniGene: Rn.9262