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  4. Recombinant Rat Caspase-3 (Casp3)

Recombinant Rat Caspase-3 (Casp3)

  • 货号：
    CSB-YP004548RA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP004548RA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP004548RA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP004548RA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP004548RA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Casp3; Cpp32Caspase-3; CASP-3; EC 3.4.22.56; Apopain; Cysteine protease CPP32; CPP-32; IRP; LICE; Protein Yama; SREBP cleavage activity 1; SCA-1) [Cleaved into: Caspase-3 subunit p17; Caspase-3 subunit p12]
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    29-175
  • 氨基酸序列
    SG IYLDSSYKMD YPEMGLCIII NNKNFHKSTG MSARNGTDVD AANLRETFMA LKYEVRNKND LTREEIMELM DSVSKEDHSK RSSFVCVILS HGDEGVIFGT NGPVDLKKLT SFFRGDYCRS LTGKPKLFII QACRGTELDC GIETD
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

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靶点详情

  • 功能：
    Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Triggers cell adhesion in sympathetic neurons through RET cleavage. Cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes. Cleaves and inhibits serine/threonine-protein kinase AKT1 in response to oxidative stress. Cleaves XRCC4 and phospholipid scramblase proteins XKR4, XKR8 and XKR9, leading to promote phosphatidylserine exposure on apoptotic cell surface.
  • 基因功能参考文献：
    1. s reinforce the potential of CASP3 as an interesting target to be explored in adult and pediatric cHL, and alert for its dual biological role in H-RS cells and tumor microenvironment. PMID: 29901224
    2. Upregulation of miR-133b-5p contributes to hypoxic preconditioning -mediated cardioprotection in cardiomyocytes, and the mechanism may be associated with inhibition of caspase-8 and caspase-3 apoptotic signaling. PMID: 29568969
    3. The protein expression values of PARP and Caspase-3 in A/R cardiomyocytes were much higher than the Control. After knockdown of lncRNA AK12348, the values decreased. PMID: 29398472
    4. These results indicate that CO2 may serve as a gasotransmitter to regulate cellular function by depressing MAPK and caspase 3 activities PMID: 28555276
    5. Omi/HtrA2-HAX-1 chain played a significant role in mitochondrial homeostasis. PMID: 27998213
    6. Studied Panax notoginseng saponins regulation of signal pathway involving mTOR/Akt, caspase-3, and miR-181b in rat model of severe acute pancreatitis. PMID: 29402262
    7. the present study demonstrated that in rats with hypoxic-ischemic brain damage , there is a positive correlation between increased apoptosis of myocardial cells and expression levels of calpain-1 and caspase-3. PMID: 28447745
    8. Results provide evidence for the first time for the activation of an autophagic BECN 1 gene in the medial temporal lobe cortex during 30 days after brain ischemia. The overexpression of the BECN 1 gene was accompanied by an early (during 2 days) downregulation of both BNIP 3 and caspase 3 genes. Next, during 7-30 days expression of both genes was significantly upregulated. PMID: 27472881
    9. Bone marrow-derived mesenchymal stem cells mitigate caspase-3 and 8-hydroxy proline induced via beta-adrenergic agonist in pulmonary injured rats. PMID: 28266775
    10. The neuroprotective effect of spermidine may be related to inhibition of the caspase 3-mediated Beclin 1 cleavage and restoration of the Beclin 1-dependent autophagy. PMID: 28383560
    11. Data suggest that increase in insulin sensitivity of extensor digitorum longus muscle following low-protein, high-carbohydrate contributes to reduced proteolysis through reduction in activity of caspase-3 and ubiquitin-proteasome system. PMID: 27239756
    12. BDNF pro-peptide regulates dendritic spines via CASP3. PMID: 27310873
    13. Results suggest that active caspase-3 appears to mediate Purkinje cells (PC) death in the shaker mutant rat. Shaker mutant rat is a unique animal model of progressive PC degeneration, due to its compartmentalization and adult onset. Thus, caspase inhibitors might be used in an attempt to inhibit PC death in order to prevent the subsequent disease and/or ameliorate its symptoms. PMID: 28040459
    14. Data indicate the role of a cathepsin-caspase 3 axis in the nuclear translocation of transcription Factor EB (TFEB) leading to lysosome biogenesis under conditions of sub-lethal oxidative stress. PMID: 28002813
    15. Data show that receptor-interacting serine-threonine kinase 3 (RIPK3) played a central role in Deltamethrin (DLM)-mediated caspase 3-independent death in liver cells. PMID: 27622324
    16. In summary, our results suggest that HO-1 can decrease H/R-induced myocardiac cell apoptosis; the mechanism may be related to the activation of the Akt signaling pathway and, furthermore, to the inhibition of the JNK/c-Jun/caspase-3 signaling pathway. PMID: 27220977
    17. the expression of the genes that encode main pro- and antiapoptotic BAX and BCL-XL proteins, as well as caspase 3 (the main effector of apoptosis), in different brain structures of rats that were selected on a high aggression towards human (or its absence) was studied. PMID: 27830683
    18. Sphallerocarpus gracilis polysaccharide protected pancreatic beta-cells from alloxan damage by several possible mechanisms, including: (1) repairing free radical damage; (2) reducing the apoptosis of pancreatic beta-cells by inhibiting the activities of caspase-3 and bax, and enhancing the activity of bcl-2; (3) stimulating insulin secretion and upregulating the pancreatic and duodenal homeobox 1 gene PMID: 27645924
    19. Morin-5'-sulfonic acid sodium salt prevents changes in the expression of apoptosis related proteins caspase 3, Bax and Bcl 2 due to mercury induced oxidative stress. PMID: 27919736
    20. metabolic stress induced dysfunction of the islet beta-cell may, in part, be due to defective protein prenylation signaling pathway. PMID: 27802439
    21. Data show that selective activation of melanocortin 4 receptor (MC4R) decreased levels of TLR4, HMGB1 and caspase-3 mRNA in liver tissues, and significantly downregulated positive rate of NF-kappaB p65 in hepatocytes. PMID: 27412936
    22. Caspase-3 expression is downregulated in rat hepatic ischemia-reperfusion injury. PMID: 27320597
    23. Cytoplasmic and nuclear immunostaining for bax, caspase-3, calpain-1 and survivin proteins were found in the endometrial epithelium and stromal cells. PMID: 26818429
    24. The expressions the TLR4, NF-kappaB, HMGB1, and caspase-3 were inhibited. PMID: 26806574
    25. Hypoxia response element Bcl2 inhibition can effectively attenuate hypoxia-induced apoptosis resistance in pulmonary microvascular endothelial cells by downregulating Bcl2 expression and upregulating caspase-3 and P53 expression. PMID: 26456506
    26. proBDNF is the predominant form of BDNF expressed in the developing rat cortex, and its expression in this brain structure correlates with the levels of active caspase-3 during perinatal development PMID: 26047381
    27. The number of active caspase-3-positive neurons was significantly increased in cerebral ischemia and this was reduced markedly by exercise preconditioning. PMID: 26012958
    28. Results revealed that betaII-spectrin was degraded into major fragments of 110/85 kDa by calpain-2 activation and 108/80 kDa by caspase-3 activation. PMID: 25270371
    29. The data obtained suggest that the increase of caspase-3 enzyme activity could affect NEP expression via proteolytic degradation of its transcription factor AICD PMID: 26983277
    30. Compared with the control group, the model and treatment groups showed significantly upregulated caspase-3, Bcl-2, and Bax mRNA and protein levels in brain tissues, but remarkably downregulated Bcl-2 mRNA and protein levels PMID: 26125843
    31. Caspase-3 inhibition alleviates pulmonary injury, thereby preventing angiogenesis as well as the development of hepatopulmonary syndrome in common bile duct ligated rats. PMID: 25113058
    32. These results suggest that OGD-R-induced hyperpermeability is ROS and caspase-3 dependent and can be regulated by their inhibitors. PMID: 24372803
    33. Neonatal testosterone acts via ESR1 in an caspase dependent manner to suppress central pattern generators required for pulsatile oxytocin release. PMID: 24518793
    34. It reduced the overexpression of the angiogenic and anti-apoptotic factors TGF-beta and Akt and improved caspase-3 expression. PMID: 25519685
    35. NGF induces removal of active caspase-3 in a lysosome-dependent manner. PMID: 24787014
    36. TNF-alpha released from Kupffer cells treated with LPS may induce hepatic naofen expression, which then stimulates hepatocellular apoptosis through activation of caspase-3. PMID: 24803807
    37. From this current study, it can be concluded that administration of curcumin improved atrazine-induced cardiotoxicity through its modulatory effect on redox status, mitochondrial function, and caspase-3 expression. PMID: 24863870
    38. Treatment with 6-OHDA increased PC12 increased caspase-3 mRNA levels by about sevenfold. CDNF pretreatment attenuated this. PMID: 24633814
    39. microwave radiation induced neural cell apoptosis via the classical mitochondria-dependent caspase-3 pathway PMID: 24688304
    40. Dual vulnerability of TDP-43 to calpain and caspase-3 proteolysis after neurotoxic conditions and traumatic brain injury. PMID: 24917042
    41. Lower survival in SHR cells correlated with increased DNA fragmentation, chromatin condensation, and caspase-3 activity, indicating apoptosis. PMID: 23220262
    42. The rat model for GA I showed mitochondrial apoptotic pathway may be involved in the GA-induced striatal lesion. PMID: 22282255
    43. The novel caspase-3 substrate Gap43 is involved in AMPA receptor endocytosis and long-term depression. PMID: 24023391
    44. Myocardium Caspase-3 mRNA, SOCS-1, SOCS-3, tumor necrosis factor-alpha (TNF-alpha) and interleukin-6 (IL-6) expression levels and Apoptosis index in myocardium ischemia-reperfusion rats, were examined. PMID: 24091940
    45. Inhibition of calpain activity also prevented caspase-3 activation. PMID: 23471945
    46. Manganese treatment induced robust Sp1 phosphorylation and increased its DNA binding activity. Overexpression of mutant Sp1 lacking phosphorylation sites attenuated Sp1's ability to stimulate manganese-induced caspase-3 promoter activity. PMID: 22975163
    47. Caspase-3 and GSK-3beta activation might participate in the dopaminergic cell death in a rat model of 6-hydroxydopamine induced Parkinson disease. PMID: 23940672
    48. Results suggest that caspase 3 and caspase 9-dependent signaling is necessary and sufficient to promote cardiomyocyte hypertrophy. PMID: 24101493
    49. S100a6/Casp gene expression between the prefrontal cortex and the cerebellum in spatial memory. PMID: 23792135
    50. The intensity of active caspase-3 in the granule cell layer of dentate gyrus is associated with cell proliferation and differentiation. PMID: 22959929

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  • 亚细胞定位：
    Cytoplasm.
  • 蛋白家族：
    Peptidase C14A family
  • 组织特异性：
    Expressed in heart, brain, liver, and muscle but not in kidney or testis.
  • 数据库链接：

    KEGG: rno:25402

    STRING: 10116.ENSRNOP00000014096

    UniGene: Rn.10562