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  4. Recombinant Rat Calreticulin (Calr)

Recombinant Rat Calreticulin (Calr)

  • 货号：
    CSB-YP004458RA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP004458RA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP004458RA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP004458RA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP004458RA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    CalrCalreticulin; CALBP; CRP55; Calcium-binding protein 3; CABP3; Calregulin; Endoplasmic reticulum resident protein 60; ERp60; HACBP
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    18-416
  • 氨基酸序列
    DPA IYFKEQFLDG DAWTNRWVES KHKSDFGKFV LSSGKFYGDQ EKDKGLQTSQ DARFYALSAR FEPFSNKGQT LVVQFTVKHE QNIDCGGGYV KLFPGGLDQK DMHGDSEYNI MFGPDICGPG TKKVHVIFNY KGKNVLINKD IRCKDDEFTH LYTLIVRPDN TYEVKIDNSQ VESGSLEDDW DFLPPKKIKD PDAAKPEDWD ERAKIDDPTD SKPEDWDKPE HIPDPDAKKP EDWDEEMDGE WEPPVIQNPE YKGEWKPRQI DNPDYKGTWI HPEIDNPEYS PDANIYAYDS FAVLGLDLWQ VKSGTIFDNF LITNDEAYAE EFGNETWGVT KAAEKQMKDK QDEEQRLKEE EEDKKRKEEE EAEDKEDEDD RDEDEDEEDE KEEDEEDATG QAKDEL
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Calcium-binding chaperone that promotes folding, oligomeric assembly and quality control in the endoplasmic reticulum (ER) via the calreticulin/calnexin cycle. This lectin interacts transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER. Interacts with the DNA-binding domain of NR3C1 and mediates its nuclear export. Involved in maternal gene expression regulation. May participate in oocyte maturation via the regulation of calcium homeostasis. Present in the cortical granules of non-activated oocytes, is exocytosed during the cortical reaction in response to oocyte activation and might participate in the block to polyspermy.
  • 基因功能参考文献：
    1. CRT might protect cardiomyocytes from apoptosis induced by angiotensin II, in which ER stress and mitochondria function are possible underlying molecular bases PMID: 29453988
    2. Exogenous CRT attenuates MR-induced ER stress-related apoptosis by suppressing CHOP-mediated apoptotic signaling pathways in MMECs. PMID: 24589181
    3. Data highlights the potential importance of CRT and other endoplasmic reticulum stress proteins in inflammatory demyelination PMID: 24252779
    4. These data demonstrate that CRT is localized at cardiomyocyte mitochondria and its mitochondrial content is increased in DCM hearts. PMID: 24813996
    5. Data suggest that Wistar Kyoto rats are unlike humans in that plasma calreticulin is not up-regulated during pregnancy in rats. Placental calreticulin is not altered by fetal growth retardation in either species (uterine vessel ligation rat model). PMID: 23972286
    6. found that at 7 days of single-prolonged stress rats had the highest calreticulin expression PMID: 23575574
    7. CRT-regulated Ca(2+)-dependent pathways are a critical molecular link between ER stress and TGF-beta fibrotic signaling PMID: 23564462
    8. The level of CRT is enhanced in nerve growth factor-stimulated differentiation of PC-12 cells through extracellular signal-regulated kinase (ERK)-dependent mitogen-activated protein kinase (MAPK) pathway. PMID: 22147490
    9. Calreticulin exits the endoplasmic reticulum (ER) to accumulate in the ER-Golgi intermediate compartment and the cis-Golgi, together with sub-optimally loaded H-2Db histocompatibility class I antigen molecules. PMID: 19851281
    10. CRT P-domain fragment CRT(221-256) constitutes an autonomous folding unit, and has a structure highly similar to that of the corresponding region in CRT(189-288) PMID: 12270713
    11. may represent a new class of high-affinity melatonin binding sites involved in some functions of the indoleamine including genomic regulation PMID: 12603316
    12. Calreticulin inhibits the MEK1,2-ERK1,2 pathway in alpha 1-adrenergic receptor/Gh-stimulated hypertrophy of neonatal rat cardiomyocytes PMID: 12648529
    13. Data show that calreticulin has an independent role in the cytosol that depends critically on its inefficient compartmentalization due to the signal sequence. PMID: 16198296
    14. CRT arginylation occurs in the cytosolic pool of mature CRT (defined by an Asp acid N-terminal) that is probably retrotranslocated from the endoplasmic reticulum PMID: 17197444
    15. Calreticulin is a high affinity ligand of GABARAP. PMID: 17916189
    16. These findings imply an important role of serine dephosphorylation and tyrosine phosphorylation on calreticulin mediated AT1 receptor mRNA stability. PMID: 18413143

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  • 亚细胞定位：
    Endoplasmic reticulum lumen. Cytoplasm, cytosol. Secreted, extracellular space, extracellular matrix. Cell surface. Sarcoplasmic reticulum lumen. Cytoplasmic vesicle, secretory vesicle, Cortical granule. Cytolytic granule.
  • 蛋白家族：
    Calreticulin family
  • 组织特异性：
    Predentin and odontoblast.
  • 数据库链接：

    KEGG: rno:64202

    STRING: 10116.ENSRNOP00000004091

    UniGene: Rn.974