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  4. Recombinant Rat Calcium/calmodulin-dependent protein kinase type II subunit alpha (Camk2a)

Recombinant Rat Calcium/calmodulin-dependent protein kinase type II subunit alpha (Camk2a)

  • 货号：
    CSB-YP004465RA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP004465RA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP004465RA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP004465RA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP004465RA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Camk2a; Calcium/calmodulin-dependent protein kinase type II subunit alpha; CaM kinase II subunit alpha; CaMK-II subunit alpha; EC 2.7.11.17
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-478
  • 氨基酸序列
    MATITCTRFT EEYQLFEELG KGAFSVVRRC VKVLAGQEYA AKIINTKKLS ARDHQKLERE ARICRLLKHP NIVRLHDSIS EEGHHYLIFD LVTGGELFED IVAREYYSEA DASHCIQQIL EAVLHCHQMG VVHRDLKPEN LLLASKLKGA AVKLADFGLA IEVEGEQQAW FGFAGTPGYL SPEVLRKDPY GKPVDLWACG VILYILLVGY PPFWDEDQHR LYQQIKAGAY DFPSPEWDTV TPEAKDLINK MLTINPSKRI TAAEALKHPW ISHRSTVASC MHRQETVDCL KKFNARRKLK GAILTTMLAT RNFSGGKSGG NKKNDGVKES SESTNTTIED EDTKVRKQEI IKVTEQLIEA ISNGDFESYT KMCDPGMTAF EPEALGNLVE GLDFHRFYFE NLWSRNSKPV HTTILNPHIH LMGDESACIA YIRITQYLDA GGIPRTAQSE ETRVWHRRDG KWQIVHFHRS GAPSVLPH
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Calcium/calmodulin-dependent protein kinase that functions autonomously after Ca(2+)/calmodulin-binding and autophosphorylation, and is involved in synaptic plasticity, neurotransmitter release and long-term potentiation. Member of the NMDAR signaling complex in excitatory synapses, it regulates NMDAR-dependent potentiation of the AMPAR and therefore excitatory synaptic transmission. Regulates dendritic spine development. Also regulates the migration of developing neurons. Phosphorylates the transcription factor FOXO3 to activate its transcriptional activity. Acts as a negative regulator of 2-arachidonoylglycerol (2-AG)-mediated synaptic signaling via modulation of DAGLA activity.
  • 基因功能参考文献：
    1. these results suggest that the CaMKII/CREB pathway in spinal neurons mediates CXCR4-facilitated pain hypersensitivity in cancer rats. PMID: 28638088
    2. CaMKIIa-GluN2B interaction had an important role in the development of L-dopa-induced dyskinesia. PMID: 30142538
    3. Storage of passive motion pattern in hippocampal CA1 region depends on CaMKII/CREB signaling pathway in a motion sickness rodent model. PMID: 28230177
    4. HCN2 is conducive to oxaliplatin-induced neuropathic pain by activating the neuronal CaMKII/CREB cascade. PMID: 29806529
    5. Data show that fluorosis could lead to the enhancement of calcium ion concentration [Ca(2+)] and the expression level of calcium/calmodulin-dependent protein kinase II alpha (CaMKIIalpha) and c-fos in the hippocampal CA3 region. PMID: 28730575
    6. WXKL may improve cardiac function and inhibit the arrhythmia by regulating the CaMK II signal transduction pathway PMID: 28573136
    7. The phosphorylation of Thr286 is associated with the early activation of CaMKII rather than the oxidation of Met281 PMID: 28536695
    8. Tau could inhibit the H2O2-induced decrease in CamKII and CaM expression at both the mRNA and protein levels. PMID: 29279520
    9. CaMKII is decreased in the hippocampus of female offspring exposed to prenatal stress that resulted in impaired spatial learning and memory. PMID: 28236215
    10. This study showed that that in diabetic rats fed with bilberry fruit, alphaCaMKII-positive nerve fibres were significantly longer when compared to the groups of diabetic and control rats; there were also statistically significant changes in the average larger diameter of alphaCaMKII-ir hippocampal neurons between groups of diabetic rats. PMID: 28865231
    11. CaMKII is a family of Ca2+-activated Ser/Thr protein kinases that mediates many intracellular responses in the brain. PMID: 26742808
    12. behavioral results, taken together with prior experiments on long Term Potentiation, strongly support a critical role of CaMKII in LTP maintenance and memory storage. PMID: 28957669
    13. Data suggest Camk2a and Prkaca exhibit substrate specificity that includes phosphorylation of Neto2 at Ser409 in C-terminal domain; Neto2 Ser409 phosphorylation inhibits synaptic targeting of Gluk1. (Camk2a = calcium-calmodulin-dependent protein kinase II, alpha subunit; Prkaca = cAMP-dependent protein kinase, alpha-catalytic subunit; Neto2 = neuropilin and tolloid-like protein 2; Gluk1 = kainate receptor Gluk1) PMID: 28717010
    14. In conclusion, our data provide evidence that damage to membrane skeleton proteins via calpain is a destructive downstream event of CaMKII activation in the setting of myocardial IR injury. PMID: 28754591
    15. these data establish a potential signaling pathway of Ca2+-CaMKII-CREB in neuronal activation. PMID: 27611779
    16. CaMKII-mediated redistribution of AIDA-1 is similar to that observed for SynGAP. CaMKII-mediated removal of two abundant PSD-95-binding proteins from the PSD core during activity is expected to initiate a molecular reorganization at the PSD. PMID: 27477489
    17. results suggest that the CaMKII signaling plays a key role in low serotonin-induced anxiety and mood disturbances, potentially through regulation of Glu/AMPA receptor subunit A1 expression in the basolateral nucleus of the amygdala. PMID: 26821292
    18. Results suggest that CaMKII controls the initiation of the memory reconsolidation process through regulation of the proteasome. PMID: 26779588
    19. The role of DRD2-CaMKIIalpha signaling is functionally reversed within the basolateral amygdala-medial prefrontal cortex pathway depending on opiate exposure state PMID: 26174594
    20. In conclusion, in addition to MLCK, CaMKII phosphorylates RLC in cardiomyocytes. PMID: 26809094
    21. The study shows that the fast decay of CaM kinase II FRET sensor signal in spines after long-term potentiation induction is not due to its dephosphorylation. PMID: 26086939
    22. These results indicate that CaMKII and ERK interact to promote activation in hypertrophy; the inhibition of CaMKII-ERK interaction offers a novel therapeutic approach to limit cardiac hypertrophy. PMID: 26110816
    23. innovative strategies for modulation of CaMKIIalpha activity in specific subpopulations of neurons could be a novel approach in therapy of diabetic trigeminal neuropathy PMID: 25701274
    24. The transient interplay between the kinase and structural functions of CaMKII during the induction of synaptic plasticity temporally gates the actin cytoskeleton. PMID: 26291163
    25. Elevated CaMKII content promotes a slow-to-fast type fibre shift in regenerating muscle but is not sufficient to stimulate mitochondrial biogenesis in the absence of an endurance stimulus. PMID: 25515219
    26. CaMKII autophosphorylation with paced contractions is regulated in an isoform and muscle type-specific fashion PMID: 25054156
    27. mTOR inhibition reduces HuD binding and promotes deadenylation of CaMKIIalpha mRNA. PMID: 25944900
    28. It involves in remodeling and generation of dendrites. PMID: 25457025
    29. VLDLR requires RasGRF1/CaMKII to alter dendritic spine formation. PMID: 25644714
    30. This study is the first description of cutaneous CaMKII expression pattern in a diabetic model. CaMKII could play a role in transformation of skin layers and contribute to cutaneous diabetic changes. PMID: 25266254
    31. Data indicate a distinct mechanism of prevention of Ca(2+)/calmodulin-dependent kinase IIalpha (CaMKIIalpha) aggregation by E3 ubiquitin-protein ligase p600 (UBR4). PMID: 25034033
    32. Angiotensin II activates PKA and CaMKII via NOX2, which results in disturbed sodium and calcium currents (via PKA) and enhanced diastolic sarcoplasmic reticulum Calcium leakage. PMID: 25073061
    33. These results suggest a potential role for CaMKII in diabetic neuropathy development PMID: 25067828
    34. Activation of the CaMKII-CREB pathway in retinal Muller cells increases HIF-1alpha and VEGF expression induced by high glucose. PMID: 22796763
    35. CaMKIIalpha and caveolin-1 cooperate to drive ligand-induced membrane delivery of the P2X3 receptor. PMID: 24755854
    36. Learning-induced enhancement of GABAA-mediated synaptic inhibition is maintained by persistent CaMKII activation. PMID: 25568108
    37. CaCMKII plays a key role in the induction of S100B expression during long-term potentiation. PMID: 25682687
    38. The specific temporospatial distribution pattern of CaMKII with NR2B might be related to the different physiological functions during postnatal development PMID: 22906554
    39. Early-life brief but recurrent hyperthermia-induced seizures (HS) caused long-term cognitive impairment and CaMKIIalpha was involved in carrying forward the signal resulting from HS PMID: 24560900
    40. Increased Camk2a autophosphorylation was found in the medial prefrontal cortex and nucleus accumbens after last cocaine self-administration session. PMID: 23953174
    41. Use of photoactivated localization microscopy determines that CaMKII mobility within spines is associated with multiple interacting proteins, even outside postsynaptic density. PMID: 24872564
    42. Released Ca(2+) dissociates preformed CaMKIIalpha from mGluR5 and meanwhile promotes active CaMKIIalpha to bind to the adjacent NMDAR GluN2B subunit, which enables CaMKIIalpha to phosphorylate GluN2B at a CaMKIIalpha-sensitive site. PMID: 24032403
    43. CaMKII activation contributes to the oxidative stress associated with neural cell death after hypoxic ischemia. PMID: 23976956
    44. we performed postembedding immunogold staining for CaMKII in quick-fixed tissue, and found that the enzyme did not concentrate primarily within the central matrix of the postsynaptic density PMID: 23749614
    45. Amelioration of impaired fear extinction in stressed rats was associated with enhancement of CamKII expression in the hippocampus. PMID: 23584669
    46. Our results suggest that the differences in the expression level of PKA and ChAT (but not of CaMKIIalpha), as well as the differences in the activation of both PKA and CaMKIIalpha, may contribute to the individual variation in learning skills PMID: 23643989
    47. Data suggest that Camk2a (calcium-calmodulin-dependent protein kinase II, alpha) regulates Drd3 (dopamine receptor D3) activation in presynaptic terminals of substantia nigra via calcium signaling. PMID: 23602989
    48. CaMKIIalpha is fine-tuned to unique bandwidths and compute input variables in an asymmetric manner. PMID: 23602566
    49. Sex differences in pain-related behavior and expression of calcium/calmodulin-dependent protein kinase II were seen in dorsal root ganglia of rats with diabetes type 1 and type 2. PMID: 23267764
    50. CaMKIIalpha contributes to induction of hyperalgesic priming, in the transition from acute to chronic pain. PMID: 23825405

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  • 亚细胞定位：
    Cell junction, synapse. Cell junction, synapse, postsynaptic density. Cell projection, dendritic spine. Cell projection, dendrite.
  • 蛋白家族：
    Protein kinase superfamily, CAMK Ser/Thr protein kinase family, CaMK subfamily
  • 数据库链接：

    KEGG: rno:25400

    UniGene: Rn.107499