Your Good Partner in Biology Research

  1. 首页
  2. 蛋白
  3. 重组蛋白
  4. Recombinant Rat Calcium/calmodulin-dependent protein kinase kinase 1 (Camkk1)

Recombinant Rat Calcium/calmodulin-dependent protein kinase kinase 1 (Camkk1)

  • 货号：
    CSB-YP004472RA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP004472RA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP004472RA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP004472RA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP004472RA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Camkk1
  • Uniprot No.：
  • 别名：
    Camkk1; Calcium/calmodulin-dependent protein kinase kinase 1; CaM-KK 1; CaM-kinase kinase 1; CaMKK 1; EC 2.7.11.17; CaM-kinase IV kinase; Calcium/calmodulin-dependent protein kinase kinase alpha; CaM-KK alpha; CaM-kinase kinase alpha; CaMKK alpha
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-505
  • 氨基酸序列
    MERSPAVCCQ DPRAELVERV AAISVAHLEE AEEGPEPASN GVDPPPRARA ASVIPGSASR PTPVRPSLSA RKFSLQERPA GSCLEAQVGP YSTGPASHMS PRAWRRPTIE SHHVAISDTE DCVQLNQYKL QSEIGKGAYG VVRLAYNERE DRHYAMKVLS KKKLLKQYGF PRRPPPRGSQ APQGGPAKQL LPLERVYQEI AILKKLDHVN VVKLIEVLDD PAEDNLYLVF DLLRKGPVME VPCDKPFPEE QARLYLRDII LGLEYLHCQK IVHRDIKPSN LLLGDDGHVK IADFGVSNQF EGNDAQLSST AGTPAFMAPE AISDTGQSFS GKALDVWATG VTLYCFVYGK CPFIDEYILA LHRKIKNEAV VFPEEPEVSE ELKDLILKML DKNPETRIGV SDIKLHPWVT KHGEEPLPSE EEHCSVVEVT EEEVKNSVKL IPSWTTVILV KSMLRKRSFG NPFEPQARRE ERSMSAPGNL LLKEGCGEGG KSPELPGVQE DEAAS
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Calcium/calmodulin-dependent protein kinase that belongs to a proposed calcium-triggered signaling cascade involved in a number of cellular processes. Phosphorylates CAMK1, CAMK1D, CAMK1G and CAMK4. Involved in regulating cell apoptosis. Promotes cell survival by phosphorylating AKT1/PKB that inhibits pro-apoptotic BAD/Bcl2-antagonist of cell death.
  • 基因功能参考文献：
    1. Site-directed mutagenesis analysis revealed that Leu(358) in CaMKKbeta/Ile(322) in CaMKKalpha confer, at least in part, a distinct recognition of AMPK but not of CaMKIalpha. PMID: 27151216
    2. CaMKKalpha and CaMKKbeta are capable of phosphorylating and activating CaMKIV in living cells. PMID: 26050738
    3. Recombinant CaM-KK alpha exhibits a high calcium/calmodulin-dependent activity with a weak autonomous activity. PMID: 11705382
    4. Translocation of CaMKI-alpha into the nucleus involves a conserved sequence located within the catalytic core. Mutation of this sequence partially blocks nuclear entry of an export-impaired mutant of CaMKI-alpha. PMID: 15147908
    5. ERK activation and neurite outgrowth in response to depolarization are mediated by CaMKK activation of CaMKI PMID: 15150258
    6. Lkb1 and CaMKKalpha function as Snf1 protein kinase kinases, in a cascade that is highly conserved in mouse and yeast PMID: 15831494
    7. CaMKII(alpha) expression levels in hippocampus of chronic epileptic rats may be related with their impaired spatial learning and memory. PMID: 18710651

    显示更多

    收起更多

  • 亚细胞定位：
    Cytoplasm. Nucleus.
  • 蛋白家族：
    Protein kinase superfamily, Ser/Thr protein kinase family
  • 组织特异性：
    Mostly expressed in the brain with higher levels in cortex and hippocampus. Lower expression levels were detected in striatum, nucleus accumbens and cerebellum (at protein level). Abundant in forebrain, weaker in cerebellum and also detected in thymus and
  • 数据库链接：

    KEGG: rno:60341

    STRING: 10116.ENSRNOP00000025009

    UniGene: Rn.4851