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  4. Recombinant Rat C-C motif chemokine 2 (Ccl2)

Recombinant Rat C-C motif chemokine 2 (Ccl2)

  • 货号：
    CSB-YP004783RA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP004783RA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP004783RA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP004783RA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP004783RA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Ccl2; Je; Mcp1; Scya2; C-C motif chemokine 2; Immediate-early serum-responsive protein JE; Monocyte chemoattractant protein 1; Monocyte chemotactic protein 1; MCP-1; Small-inducible cytokine A2
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    24-148
  • 氨基酸序列
    QPDAVNA PLTCCYSFTG KMIPMSRLEN YKRITSSRCP KEAVVFVTKL KREICADPNK EWVQKYIRKL DQNQVRSETT VFYKIASTLR TSAPLNVNLT HKSEANASTL FSTTTSSTSV EVTSMTEN
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Acts as a ligand for C-C chemokine receptor CCR2. Signals through binding and activation of CCR2 and induces a strong chemotactic response and mobilization of intracellular calcium ions. Exhibits a chemotactic activity for monocytes and basophils but not neutrophils or eosinophils. Plays an important role in mediating peripheral nerve injury-induced neuropathic pain. Increases NMDA-mediated synaptic transmission in both dopamine D1 and D2 receptor-containing neurons, which may be caused by MAPK/ERK-dependent phosphorylation of GRIN2B/NMDAR2B.
  • 基因功能参考文献：
    1. Study shows that the unique combination of monocyte chemoattractant protein-1 andsecreted ectodomain of sialic acid-binding Ig-like lectin-9 ameliorates rat acute liver failure by inhibiting hepatocellular apoptosis and promoting liver regeneration through the induction of anti-inflammatory/tissue-repairing M2 macrophages. PMID: 28272428
    2. Light exposure led to apoptosis of photoreceptors, which expressed CCL2, accelerating an inflammation-mediated cascade by activating and attracting microglia and monocytes and promoting their secretion of CCL2 in the injured position. PMID: 29142497
    3. MCP-1/sSiglec-9 regenerates peripheral nerves by inducing tissue-repairing M2 macrophages. PMID: 27862629
    4. Tibial fracture surgery in a rat model promoted CCL2 upregulation in activated astrocytes, increased CCR2 expression in activated microglia, and induced deficits in learning and memory. Site-directed pre-injection of RS504393, a CCR2 antagonist, inhibited this effect by reducing microglial activation, M1 polarization, inflammatory cytokines, and neuronal injury and death and improving cognitive function. PMID: 28587818
    5. Low wall shear stress concomitant with turbulent flow contributes to sustained expression of MCP-1 in endothelial cells and presumably plays a role in facilitating macrophage infiltration and exacerbating inflammation, which leads to enlargement or rupture of intracranial aneurysms. PMID: 27160403
    6. An association between serum MCP-1 and SDF-1 concentrations and behavioral manifestations of neuropathic pain. PMID: 26974138
    7. The increase of MCP-1 accompanying the development of hypoxic pulmonary hypertension probably originates from other sources than alveolar macrophages. PMID: 27429111
    8. Dexmedetomidine is a potent suppressor of MCP-1 in astrocytes induced with lipopolysaccharide through alpha2A-adrenergic receptors, which potentially explains its beneficial effects in the treatment of delirium by attenuating neuroinflammation. PMID: 28286770
    9. This study showed that CCL2, NF-kappaB1, RAC2, FCER1G and C1Q may contribute to the generation of neuropathic pain after sciatic nerve injury via immune and defense pathways. PMID: 28033741
    10. Results demonstrate that prenatal high-fat diet exposure greatly disturbs the functioning of the CCL2 chemokine system in embryonic hypothalamic neurons, reducing its endogenous levels and ability to promote the migration of neurons and their expression of orexigenic peptides PMID: 26979053
    11. These results demonstrate that high glucose enhances MCs proliferation and MCP-1 expression by activating the ROS/NF-kappaB pathway and can be inhibited by EE. Our findings provide a new perspective for the clinical treatment of diabetic nephropathy . PMID: 26644089
    12. CCL2 mRNA is increased in adolescent brain, but not adult brain during withdrawal from chronic alcohol. PMID: 26556523
    13. Our study showed that hyperuricemia accelerates renal progression via a mechanism linked to high MCP-1 which may mediate the inflammation reaction of renal diseases induced by hyperuricemia. PMID: 25830624
    14. Report a dual effect of n-3 PUFAs on the regulation of Mcp-1 expression in vascular smooth muscle. PMID: 26402697
    15. Study identifies an innate neuronal TLR4 signal that functions in central nucleus of the amygdala and ventral tegmental area to regulate the initiation of excessive alcohol drinking through MCP-1 neurotransmission likely at the level of dopamine PMID: 25567426
    16. The expressions of MCP-1 proteins began to increase gradually after balloon injury and were obviously detected in the VSMC on the 4th and 7th day, until 14th day when it was still detected clearly in the proliferating intima. PMID: 26062549
    17. TGFalpha signaling stimulates cartilage degradation via a CCL2-dependent mechanism, but pharmacologic inhibition of the TGFalpha-CCL2 axis reduces experimental posttraumatic osteoarthritis progression in vivo. PMID: 26138996
    18. Inhibition of monocyte chemoattractant peptide-1 decreases secondary spinal cord injury PMID: 25672988
    19. Stromal cell-derived CCL2 (in contrast to CCL2 immune cell-derived) tightly controls monocyte/macrophage recruitment into the lesion and plays a major role in the development of neuropathic pain. PMID: 25449579
    20. Inhibition of S1PR2 down-regulate the expressions of SphK1 and MCP-1 in glomerular mesangial cells under high glucose. PMID: 25575056
    21. findings suggest that the LPS-miR-34a-CCL22 axis may contribute to the recruitment of Tregs in periapical lesions, providing a potential therapeutic target for controlling this disease PMID: 25817785
    22. role of CCL2 in monocyte trafficking into the retina in diabetic retinopathy PMID: 25329075
    23. aging can reduce the expression of CINC and MCP-1 mRNA in lung tissues, and reduce the infiltration of neutrophils and monocyte-macrophages induced by CINC and MCP-1. This might lead to increased risk of pneumonia in elderly patients. PMID: 24464584
    24. Serum concentration of chemokine (c-c motif) ligand 2, L-selectin and intra cellular adhesion molecule-1 also increased with growing tumor PMID: 25667449
    25. PKC-NF-kappaB are involved in CCL2-induced elevation of Nav1.8 current density by promoting the phosphorylation of Nav1.8 and its expression. PMID: 24724624
    26. IgE alone is sufficient to stimulate basophils to increase expression of MCP-1, which in turn might contribute to the inflammatory response. PMID: 24980870
    27. Results suggest that CCL2/CCR2 in the dorsal root ganglion and spinal cord is involved in the maintenance of lumbar disc herniation-induced pain PMID: 24462503
    28. MCP-1 induces persistent muscle hyperalgesia and latent chronic sensitization. PMID: 24637038
    29. This study indicates that TNF-alpha was released from the activated microglia induced by retinal neuronal MCP-1 via the p38, ERK, and NF-kappaB pathways, but not c-Jun N-terminal kinase. PMID: 24826069
    30. Epithelium-derived exosomal ATF3 RNA attenuates ischemia-reperfusion induced kidney injury by inhibiting MCP-1 gene transcription. PMID: 24420912
    31. Hesperetin-7-O-glucuronide decreased hydrogen peroxide-induced intracellular adhesion molecule-1 and monocyte chemoattractant protein-1 mRNA expression in rat aortic endothelial cells. PMID: 23831969
    32. osteoblast and osteocyte MCP-1 expression is an important mediator for the anabolic effects of PTH on bone. PMID: 23519994
    33. TGF-beta1 exhibits pro-inflammatory effects through the upregulation of MCP-1 in peritoneal fibrosis. Smad7 inhibits TGF-beta1 induced MCP-1 upregulation through a MAPK/p38-dependent pathway. PMID: 23242502
    34. An increase in dentate MCP-1 is associated with seizure-induced aberrant cell migration of neuronal progenitor cells. PMID: 23339567
    35. These data support an important role for MCP-1 in the early phase of stress fracture repair and activated remodeling PMID: 23460341
    36. The present study suggests that the expressed on endothelial cells and astrocytes are responsible for the recruitment of brain Iba1(+) /NG2(+) cells progenitors during the acute phase of stroke. PMID: 23400803
    37. Report functional roles for CCL2/CCR2 signaling at the level of the urinary bladder in reducing voiding frequency and somatic sensitivity following CYP-induced cystitis. PMID: 23594826
    38. MCP-1 and other bone matrix proteins might be involved in the formation of vascular lesions of diabetic nephropathy. PMID: 22812249
    39. glucocorticoid-mediated repression of IL-1beta-induced CCL2 gene transcription and protein secretion occurs in part through the upregulation of the MKP-1 gene PMID: 23056550
    40. High-dose losartan can effectively control blood pressure, reduce renal damage and fibrosis, and inhibit MCP1 and TGF-beta(1) expression in rats with unilateral ureteral obstruction. PMID: 21868333
    41. These results indicated that the increase in urinary CCL2 is associated with tubular injury and serves as a sensitive and noninvasive marker for the early detection of cisplatin-induced tubular injury. PMID: 23291264
    42. spinal neuronal MCP-1 is a key mediator of the spinal microglial activation and is involved in morphine antinociceptive tolerance PMID: 23187120
    43. MCP-1 upregulation in IC/BPS is one of possible contributing factors inducing histamine release from mast cells . PMID: 23049171
    44. Peptide fragment 29-40 of amino acid sequence of monocyte chemoattractant protein-1 (MCP-1) stimulates monocyte migration in vivo and facilitates wound healing. PMID: 23129286
    45. Xiongshao capsule could enhance the protective effect of ischemic postconditioning by inhibiting MCP-1 and TNF-alpha in rats with myocardial reperfusion injury. PMID: 21302491
    46. Upregulation of MCP-1 began during the early stage of DR and increased with the development of the disease. PMID: 23010641
    47. CCL2-CCR2 signaling cascades play a role in neuron-glia-cytokine interactions and the descending facilitation of neuropathic pain. PMID: 22466130
    48. These studies define new roles for glucocorticoid receptor, Y-box binding protein 1, and RNase UK114 in the formation of a molecular complex that degrades MCP-1 mRNA. PMID: 22801372
    49. MCP-1 through CCR2 was implicated in Angiotensin II-stimulated proliferation of vascular smooth muscle cells PMID: 22527942
    50. Altered pulmonary gene expression of PPARgamma and MCP-1 during late gestation may impair lung development and maturation, contributing to pulmonary hypoplasia in the nitrofen-induced congenital diaphragmatic hernia model. PMID: 22595559

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  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Intercrine beta (chemokine CC) family
  • 数据库链接：

    KEGG: rno:24770

    STRING: 10116.ENSRNOP00000009448

    UniGene: Rn.4772