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  4. Recombinant Rat BDNF/NT-3 growth factors receptor (Ntrk2), partial

Recombinant Rat BDNF/NT-3 growth factors receptor (Ntrk2), partial

  • 货号：
    CSB-YP720531RA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP720531RA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP720531RA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP720531RA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP720531RA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Ntrk2; Trkb; BDNF/NT-3 growth factors receptor; EC 2.7.10.1; Neurotrophic tyrosine kinase receptor type 2; TrkB tyrosine kinase; Trk-B
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Receptor tyrosine kinase involved in the development and the maturation of the central and the peripheral nervous systems through regulation of neuron survival, proliferation, migration, differentiation, and synapse formation and plasticity. Receptor for BDNF/brain-derived neurotrophic factor and NTF4/neurotrophin-4. Alternatively can also bind NTF3/neurotrophin-3 which is less efficient in activating the receptor but regulates neuron survival through NTRK2. Upon ligand-binding, undergoes homodimerization, autophosphorylation and activation. Recruits, phosphorylates and/or activates several downstream effectors including SHC1, FRS2, SH2B1, SH2B2 and PLCG1 that regulate distinct overlapping signaling cascades. Through SHC1, FRS2, SH2B1, SH2B2 activates the GRB2-Ras-MAPK cascade that regulates for instance neuronal differentiation including neurite outgrowth. Through the same effectors controls the Ras-PI3 kinase-AKT1 signaling cascade that mainly regulates growth and survival. Through PLCG1 and the downstream protein kinase C-regulated pathways controls synaptic plasticity. Thereby, plays a role in learning and memory by regulating both short term synaptic function and long-term potentiation. PLCG1 also leads to NF-Kappa-B activation and the transcription of genes involved in cell survival. Hence, it is able to suppress anoikis, the apoptosis resulting from loss of cell-matrix interactions. May also play a role in neutrophin-dependent calcium signaling in glial cells.
  • 基因功能参考文献：
    1. The above results suggest that rutin preconditioning ameliorates cerebral I/R injury in OVX rats through ER-mediated BDNF-TrkB and NGF-TrkA signaling. PMID: 29420916
    2. Uregulated mRNA levels of BDNF and TrkB in the brain might be essential for antidepressant-like activity of memantine in stress exposed rats. PMID: 29680698
    3. Results indicate the BDNF system (precursor proBDNF, BDNF pro-peptide, BDNF mRNA levels, as well as TrkB and p75(NTR) receptors) in the brain of rats selectively bred for more than 85 generations for either the high level or the lack of fear-induced aggressive is extremely different at all levels, from transcription to reception, suggesting role of BDNF system in the development of highly aggressive phenotype. PMID: 29425916
    4. Reduced Bdnf/trkB signaling in the hippocampus of vulnerability (Roman low-avoidance) and resistance (Roman high-avoidance) rats may contribute to their more pronounced vulnerability to Stress-induced Depression. PMID: 29075579
    5. Results indicate that the hippocampal brain-derived neurotrophic factor/TrkB signaling pathway is activated by multiple electroconvulsive seizure treatments despite the ligand-induced down-regulation of the full-length TrkB receptor. PMID: 28890399
    6. findings support a role for TrkB receptors in regulating basal and stress-induced social, cognitive and motivational behavior PMID: 28705440
    7. This study demonstrated that Aberrant gene expression of tropomyosin receptor kinase B (TrkB) in the hippocampus (HPC)-prefrontal cortex (PFC) network was observed in binge eating prone rats. PMID: 28821448
    8. clarification of diverse distribution of three TrkB isoforms in rat multiple tissues PMID: 28757509
    9. We find that BDNF mediates NMDAR potentiation through activation of TrkB and phosphorylation of the GluN2B subunit by the Src-family kinase Fyn PMID: 27926876
    10. Treatment with the TrkB agonist at a low dose, but not at a high dose, prevented the decrease of excitatory glutamatergic synapses, and both doses increased the density of inhibitory synapses after sciatic nerve injury. TrkB inactivation counteracted only some of the positive effects exerted by exercise after nerve injury, such as maintenance of excitatory synapses surrounding motoneurons. PMID: 27984178
    11. Phosphorylation of tyrosine receptor kinase B in the dorsal striatum and dorsal hippocampus is associated with response learning in a water plus maze. Findings suggest that activation of TrkB in a sparse arrangement of cells may be associated with faster acquisition of a response task. PMID: 28004953
    12. Co-immunoprecipitation and biochemical fractionation data suggested that p75 TM stimulates TrkB phosphorylation at the cell membrane. PMID: 28821608
    13. These data indicate that endogenous BDNF signalling in the nucleus tractus solitarius (NTS) is integral for the maintenance of baroreflex sensitivity and that BDNF/TrkB signalling is impaired in the NTS in the chronic heart failure state. PMID: 27151332
    14. Together, these data implicate trkB signaling in the symptomatic efficacy and disease-modifying potential of STN DBS. PMID: 28607168
    15. inhibition of Sig-1 R prevented cocaine-induced TrkB activation in hippocampal neurons. PMID: 27735948
    16. Results demonstrate that Creb1, Bdnf and TrkB expression exhibits endogenously-driven rhythms in the rat PFC. In addition, BMAL1 protein levels oscillate on a circadian basis in the same brain area PMID: 27771283
    17. The BDNF/TrkB pathway may participate in the epileptogenesis through modulating the biological effect of GLT-1 in the glial cells of PTZ kindling rats, though the specific mechanisms require further investigations. PMID: 27438618
    18. It has been shown that ischemia influenced the expression of neurotrophins (Bdnf, Ngf, Nt-3) and their receptors (TrkB, TrkA, TrkC, p75) in brain structures outside the lesion focus, including the contralateral hemisphere. PMID: 27830679
    19. Results highlight differences in the involvement of TrkB in the chronic effects of an antidepressant drug and a vagal nerve stimulation therapy as well as its role in acute versus chronic effects of desipramine PMID: 26899129
    20. Data suggest that BDNF regulates postnatal carotid body growth via TrkB but does not influence the development of glomus cell O2 sensitivity PMID: 26222433
    21. Brain ischaemia induces shedding of a BDNF-scavenger ectodomain from TrkB receptors by excitotoxicity activation of metalloproteinases and gamma-secretases. PMID: 26712630
    22. Study showed that complex relationships exist between BDNF/TrkB gene expression and interneuron marker gene expression that appear to be dependent on the presence of testosterone at adolescence PMID: 26088421
    23. Intravitreous injection of erythropoietin can increase BDNF and TrkB expression in diabetes rat retina. PMID: 26339375
    24. The study provides further insight into the mechanisms of regulation of STEP61 and also offers a molecular basis for the Zn(2+)-induced sustained activation of ERK2. PMID: 26574547
    25. The results also demonstrate that synaptic activity-induced muscle contraction promotes changes in presynaptic nPKCepsilon through the brain-derived neurotrophic factor (BDNF)-mediated tyrosine kinase receptor B (TrkB) signaling. PMID: 25761522
    26. Results suggest that testosterone does not down-regulate neurogenesis at adolescence via overall changes in BDNF or TrkB expression; however, BDNF/TrkB mRNA appears to have a greater link to cell proliferation in the presence of circulating testosterone PMID: 25886766
    27. Data suggest the pivotal role of BDNF receptor (TrkB) signaling in cognitive performance after brain injury. PMID: 25661191
    28. TRKB receptor expression is increased in corpus striatum of methamphetamine craving animals. PMID: 26019338
    29. Results indicate that TrkB is an intermediator for the trophic and toxicity-exacerbating effects of BDNF against cell viabilities at non-cytotoxic and cytotoxic glutamate concentrations, respectively PMID: 24934279
    30. Long-term potentiation (LTP) consolidation in the dentate gyrus of live rodents requires sustained (hours) BDNF-TrkB signaling and from TrkB to MAP-kinase-interacting kinase. PMID: 25453757
    31. These results suggest that a stochastic or random expression pattern for BDNF and their receptors might be set in each of the CMEC to response requirement of the time and spatial change, regulation or pathophysiological change. PMID: 25226925
    32. Study shows that TrkB is expressed by several pools of oligodendrocyte lineage cells in the adult spinal cord PMID: 25072185
    33. Alterations in TrkB.FL signalling may be regulated via TrkB.T receptors. Upregulation of TrkB.FL signalling suppresses epileptiform discharges in the rat hippocampal neuronal model of spontaneous recurrent epileptiform discharges model. PMID: 24830751
    34. The expression of BDNF and of its TRKB and p75NT receptors correlates with the proliferation rate of biliary tree during bile duct ligation. PMID: 25665281
    35. Findings suggest that both BDNF and its receptor TrkB are expressed in rodent and human placenta being regulated during pregnancy. PMID: 24372023
    36. adenosine A2A receptor activation increases the levels of TrkB receptors in neuronal lipid rafts and potentiates BDNF-induced TrkB phosphorylation in these membrane microdomains. PMID: 24271058
    37. Neuronal maturation required for 7,8-DHF but not for BDNF to elicit the full spectrum of TrkB signaling cascades. PMID: 25143381
    38. Results suggest that the influence of BDNF TrkB receptors upon hippocampal Long-Term Depression is under the strict control of A(2A)Rs activation, through a mechanism that requires the cAMP/PKA transducing system PMID: 24361450
    39. TrkB (and PLC-gamma) was decreased in intrauterine growth retardation. PMID: 24599793
    40. The BDNF/TrkB signaling pathway is involved in heat hyperalgesia mediated by Cdk5 in rats. PMID: 24465591
    41. Phosphorylated TrkB expression is elevated in the cortex following 7,8-dihydroxyflavone treatment. PMID: 24045895
    42. IGF-1 might relieve the dideoxycytidine-induced toxicity of TrkA- and TrkB-, but not TrkC-expressing dorsal root ganglion neurons. PMID: 24198040
    43. Chronic administration of vagal nerve stimulation activated signaling pathways downstream of BDNF receptor in brain. PMID: 24103847
    44. juvenile rats (aged 26-28 days) exhibited significantly elevated basal BDNF expression and activation of full-length TrkB (TrkBfull) in nucleus accumbens compared to their adult counterparts, as evidenced by increased TrkBfull phosphorylation. PMID: 24021607
    45. the BDNF-TrkB signaling pathway is mainly involved in the early phases of epileptogenesis, but not the synchronized neuronal burst activity associated with epileptic seizure in the pentylenetetrazole animal model PMID: 23550026
    46. Data suggest that neurotrophins can promote cell viability through Trk receptors in a manner depending on basal neutral sphingomyelinase (nSMase) but not through SMase activity enhancement. PMID: 24316227
    47. Data indicate that delta-opioid receptor (DOR)activation rescues TrkB signaling by reversing ischemia/reperfusion induced decrease in the full-length TrkB receptor and reduces brain injury in ischemia/reperfusion. PMID: 23844255
    48. Brain-derived neurotrophic factor loop 4 dipeptide mimetic GSB-106 activates TrkB, Erk, and Akt and promotes neuronal survival in vitro. PMID: 23975404
    49. Neuronal TRKB trophic function is ablated by MMP9-mediated degradation in the diabetic brain. PMID: 23867621
    50. In a permanent middle cerebral artery occlusion model, ischemia decreased NT-4 and trkB expression. Immunohistochemistry showed their immunoreactivities around the region of the ischemic area. PMID: 23526925

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  • 亚细胞定位：
    Cell membrane; Single-pass type I membrane protein. Endosome membrane; Single-pass type I membrane protein. Early endosome membrane. Cell projection, axon. Cell projection, dendrite. Cytoplasm, perinuclear region. Cell junction, synapse, postsynaptic density.
  • 蛋白家族：
    Protein kinase superfamily, Tyr protein kinase family, Insulin receptor subfamily
  • 组织特异性：
    Widely expressed in the central and peripheral nervous system. The different forms are differentially expressed in various cell types. Isoform T2 is primarily expressed in neurons.
  • 数据库链接：

    KEGG: rno:25054

    STRING: 10116.ENSRNOP00000045635

    UniGene: Rn.11246