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  4. Recombinant Rat Arginase-1 (Arg1)

Recombinant Rat Arginase-1 (Arg1)

  • 货号：
    CSB-YP002005RA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP002005RA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP002005RA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP002005RA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP002005RA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Arg1
  • Uniprot No.：
  • 别名：
    Arg1Arginase-1; EC 3.5.3.1; Liver-type arginase; Type I arginase
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-323
  • 氨基酸序列
    MSSKPKPIEI IGAPFSKGQP RGGVEKGPAA LRKAGLVEKL KETEYNVRDH GDLAFVDVPN DSPFQIVKNP RSVGKANEQL AAVVAETQKN GTISVVLGGD HSMAIGSISG HARVHPDLCV IWVDAHTDIN TPLTTSSGNL HGQPVAFLLK ELKGKFPDVP GFSWVTPCIS AKDIVYIGLR DVDPGEHYII KTLGIKYFSM TEVDKLGIGK VMEETFSYLL GRKKRPIHLS FDVDGLDPVF TPATGTPVVG GLSYREGLYI TEEIYKTGLL SGLDIMEVNP TLGKTPEEVT RTVNTAVALT LSCFGTKREG NHKPETDYLK PPK
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

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靶点详情

  • 功能：
    Key element of the urea cycle converting L-arginine to urea and L-ornithine, which is further metabolized into metabolites proline and polyamides that drive collagen synthesis and bioenergetic pathways critical for cell proliferation, respectively; the urea cycle takes place primarily in the liver and, to a lesser extent, in the kidneys.; Functions in L-arginine homeostasis in nonhepatic tissues characterized by the competition between nitric oxide synthase (NOS) and arginase for the available intracellular substrate arginine. Arginine metabolism is a critical regulator of innate and adaptive immune responses. Involved in an antimicrobial effector pathway in polymorphonuclear granulocytes (PMN). Upon PMN cell death is liberated from the phagolysosome and depletes arginine in the microenvironment leading to suppressed T cell and natural killer (NK) cell proliferation and cytokine secretion. In group 2 innate lymphoid cells (ILC2s) promotes acute type 2 inflammation in the lung and is involved in optimal ILC2 proliferation but not survival. Plays a role in the immune response of alternatively activated or M2 macrophages in processes such as wound healing and tissue regeneration, immune defense against multicellular pathogens and parasites, and immune suppression and allergic inflammation; the regulatory outcome seems to be organ specific. In tumor-infiltrating dendritic cells (DCs) and myeloid-derived suppressor cells (MDSCs) plays a role in suppression of T cell-mediated antitumor immunity.
  • 基因功能参考文献：
    1. Arg1 expression, which is abundant in polarized M2 cells, is associated with strain/genotype differences from different pathways. PMID: 25872571
    2. Arginase promotes endothelial dysfunction and hypertension in obesity by reducing arginine bioavailability. PMID: 25557182
    3. Lower activity and expression levels of iNOS and higher activity and expression levels of arginase-1 in rat alveolar macrophages were found to be linked to the susceptibility of T. gondii infection in these cells PMID: 23691079
    4. Diabetes-induced increases in arginase I were involved in the diabetes-induced impairment of retinal blood flow by a mechanism involving vascular endothelial cell dysfunction. PMID: 23232640
    5. Report utility of arginase-1 for monitoring drug-induced liver injury in rat model. PMID: 22872058
    6. Arginase and methylated arginine derivatives appear to be essential modulators of the inflammatory response in the acute phase of a multiple sclerosis model. PMID: 22507752
    7. This study suggested that the increased level of arginase-1 in SCI is associated with an increase in macrophages and reactive astrocytes, possibly contributing to the modulation of inflammation during the course of SCI. PMID: 22325098
    8. we postulate that the increased level of arginase-1, which is partly from M2 macrophages, contributes to the modulation of neuroinflammation in experimental autoimmune encephalomyelitis lesions PMID: 22483960
    9. Arginase contributes significantly to L-proline supply for collagen synthesis in rat fibroblasts, in which arginase I is the predominant isoenzyme PMID: 20107769
    10. These findings reveal the basis for thrombin induction of endothelial arginase I and indicate that arginase inhibition may be an attractive therapeutic alternative in the setting of arterial thrombosis and its associated endothelial dysfunction. PMID: 20032511
    11. crystal structure: arginase-boronic acid complex highlights a physiological role in erectile function PMID: 10542097
    12. Regulates low-level NO production by neuronal NOS (nNOS), most likely by competing for L-arginine. PMID: 11829529
    13. Mechanisms involved in substrate binding and catalysis are elucidated via synthesis and evaluation of alternative substrate. PMID: 12020133
    14. The upregulation of the arginase expression in wound derived fibroblasts underlines the distinct regulation of l-arginine metabolism in WFBs. PMID: 12069499
    15. Total arginase activity and arginase I and II protein expression did not differ between the young and aged groups in the prefrontal cortex. PMID: 15013576
    16. The highest affinity inhibitorsof Arg1 displace the metal-bridging hydroxide ion (and sometimes occupy a Mn(2+)(A) site found vacant in the native enzyme) and maintain a conserved array of hydrogen bonds with their alpha-amino and -carboxylate groups. PMID: 15248756
    17. Arg I plays a critical role in the pathobiology of age-related endothelial dysfunction. PMID: 16380531
    18. Arginase I protein was undetectable in the non-pregnant myometrium and up-regulated at term gestation, contributing to enhanced overall arginase activity at term gestation. PMID: 16735458
    19. Arginase acts as central regulator of trophic factor-deprived motor neuron survival by suppressing nitric oxide production and consequent peroxynitrite toxicity. Resistance of motor neurons to trophic factor deprivation may result from increased arginase. PMID: 16914676
    20. Thus, in addition to enhancing the expression of Arg I and spermine in repaired spinal cords, our treatment may recruit activated macrophages and create a more favorable environment for axonal regrowth. PMID: 17418108
    21. diabetes-induced impairment of vasorelaxation to acetylcholine was correlated with increases in reactive oxygen species and arginase activity and arginase I expression in aorta and liver. PMID: 17967788
    22. Arginase promotes neointima formation in rat injured carotid arteries. PMID: 19164802
    23. upregulation of Arg I and increased synthesis of polyamines play an important role in the conditioning lesion effect, and spermidine is sufficient to promote optic nerve regeneration in vivo PMID: 19641117
    24. Data indicate that iNOS-dependent S-nitrosylation of arginase 1 and the increase in arginase activity lead to eNOS uncoupling, contributing to the nitroso-redox imbalance, endothelial dysfunction, and vascular stiffness observed in vascular aging. PMID: 19661445
    25. Limits iNOS-mediated NO synthese in macrophages, probably by limiting L-arginine availability for iNOS (substrate competition) PMID: 9179379

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  • 亚细胞定位：
    Cytoplasm. Cytoplasmic granule.
  • 蛋白家族：
    Arginase family
  • 组织特异性：
    Detected in liver (at protein level).
  • 数据库链接：

    KEGG: rno:29221

    STRING: 10116.ENSRNOP00000017911

    UniGene: Rn.9857