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  4. Recombinant Rat Angiotensinogen (Agt)

Recombinant Rat Angiotensinogen (Agt)

  • 货号：
    CSB-YP001463RA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP001463RA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP001463RA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP001463RA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP001463RA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Agt; Serpina8Angiotensinogen; Serpin A8) [Cleaved into: Angiotensin-1; Angiotensin 1-10; Angiotensin I; Ang I); Angiotensin-2; Angiotensin 1-8; Angiotensin II; Ang II); Angiotensin-3; Angiotensin 2-8; Angiotensin III; Ang III; Des-Asp[1]-angiotensin II); Angiotensin-4; Angiotensin 3-8; Angiotensin IV; Ang IV); Angiotensin 1-9; Angiotensin 1-7; Angiotensin 1-5; Angiotensin 1-4]
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    25-477
  • 氨基酸序列
    DRVYIH PFHLLYYSKS TCAQLENPSV ETLPEPTFEP VPIQAKTSPV DEKTLRDKLV LATEKLEAED RQRAAQVAMI ANFMGFRMYK MLSEARGVAS GAVLSPPALF GTLVSFYLGS LDPTASQLQV LLGVPVKEGD CTSRLDGHKV LTALQAVQGL LVTQGGSSSQ TPLLQSTVVG LFTAPGLRLK QPFVESLGPF TPAIFPRSLD LSTDPVLAAQ KINRFVQAVT GWKMNLPLEG VSTDSTLFFN TYVHFQGKMR GFSQLTGLHE FWVDNSTSVS VPMLSGTGNF QHWSDAQNNF SVTRVPLGES VTLLLIQPQC ASDLDRVEVL VFQHDFLTWI KNPPPRAIRL TLPQLEIRGS YNLQDLLAQA KLSTLLGAEA NLGKMGDTNP RVGEVLNSIL LELQAGEEEQ PTESAQQPGS PEVLDVTLSS PFLFAIYERD SGALHFLGRV DNPQNVV
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Essential component of the renin-angiotensin system (RAS), a potent regulator of blood pressure, body fluid and electrolyte homeostasis.; acts directly on vascular smooth muscle as a potent vasoconstrictor, affects cardiac contractility and heart rate through its action on the sympathetic nervous system, and alters renal sodium and water absorption through its ability to stimulate the zona glomerulosa cells of the adrenal cortex to synthesize and secrete aldosterone.; stimulates aldosterone release.; is a ligand for the G-protein coupled receptor MAS1. Has vasodilator and antidiuretic effects. Has an antithrombotic effect that involves MAS1-mediated release of nitric oxide from platelets.
  • 基因功能参考文献：
    1. these results suggest that 2K1C rats are more responsive to the natriorexigenic effects of ANG II, whereas other responses to ANG II are not modified. PMID: 29305157
    2. CGRP has an anti-inflammatory effect in vascular smooth muscle cells (VSMCs) stimulated by Ang II; ADAM17 is involved in the protective effect of CGRP against Ang II-induced inflammation via the EGFR-ERK1/2 pathway in VSMCs PMID: 30046277
    3. Ang II induced epithelial-mesenchymal transition in renal tubular epithelial cells via Tgfb1. PMID: 29746862
    4. The s provide evidence demonstrating the critical role of Angiotensin II/TRPC6 axis in the control of glomeruli function, which is likely important for the development of glomerular diseases. PMID: 28331185
    5. Ang II down-regulates KLF15 expression via the AT1 receptor, and KLF15 is likely to inhibit Ang II-induced CTGF expression by repressing the recruitment of the co-activator P/CAF to the CTGF promoter. PMID: 29179208
    6. results established that A20 is involved in the renoprotective effect by calcitriol via negatively modulating the NF-kappaB pathway and necroptotic pathway in AngII-induced renal injury. PMID: 28744711
    7. The mRNA expression of cardiac AngII was significantly increased after the 8week exercise period, while AT1R was significantly decreased. PMID: 28656283
    8. ACE2Ang (17)MasR axis may present a potential protective role in the development of myocardial remodeling. PMID: 28656296
    9. CaSR activation results in intracellular calcium release, MEK1/2 pathway phosphorylation, autophagy activation and collagen formation induced by AngII in cardiac fibroblasts. PMID: 29452090
    10. Ang II is involved in the degradation of myosin light chain kinase in pressure overload-induced cardiac hypertrophy and that this process was mediated by MMP9. PMID: 29262413
    11. Data suggest that increases in adrenal blood flow induced by Ang II (angiotensin II)/Agtr2 signaling are mediated by release of nitric oxide and EETs (epoxyeicosatrienoic acids); increases in adrenal blood flow with ACTH (adrenocorticotropic hormone)/Mc2r receptor signaling are mediated by EETs only. (Agtr2 = angiotensin II receptor; Mc2r = ACTH receptor) PMID: 29140411
    12. Ang II increased Csk expression and induced podocyte apoptosis, stimulated Csk translocation and binding to Caveolin-1. PMID: 27225249
    13. Major components of the renin angiotensin system are located in striatal projection neurons, and striatal astrocytes and microglial cells of rats. PMID: 28161727
    14. Ang-(1-5) is an active mediator of renin-angiotensin system to stimulate ANP secretion via Mas R and PI3K-Akt-NOS pathway. PMID: 27660028
    15. Smad7 influenced NF-kappaB expression by regulating reactive oxygen species generation, and induced glomerular mesangial cell apoptosis to counter the Ang II-promoted proliferation. PMID: 28988741
    16. The activation of Ang II system in nucleus tractus solitarii may impair blood pressure baroreflex, and contribute to autonomic dysreflexia after spinal cord injury. PMID: 28742157
    17. Enhanced expression and phosphorylation of Sirt7 activates smad2 and ERK signaling and promotes the cardiac fibrosis differentiation upon angiotensin-II stimulation. PMID: 28582407
    18. Ang II upregulates the expression of Kv1.5, SAP97 and TGF-beta1 in neonatal rat atrial myocytes. PMID: 25451261
    19. MiRNA-29a and AGT mRNA expressions were negatively correlated in a rat model of diabetic retinopathy (DR). Overexpression of miRNA-29a could down-regulate AGT expression, thereby preventing the development of DR. PMID: 28189547
    20. Results indicate that aldosterone (Aldo)-mineralocorticoid receptor (MR) signaling in the subfornical organ (SFO) is needed for the activation of angiotensin II-AT1R-reactive oxygen species signaling from the SFO to the nucleus (PVN)and rostral ventrolateral medulla. Activation of Aldo-MR signaling from the SFO to the supraoptic nucleus may enhance AT1R dependent activation of pre-sympathetic neurons in the PVN. PMID: 27163380
    21. Ang-(1-7) improved liver fibrosis by regulating NLRP3 inflammasome activation induced by Ang II-mediated reactive oxygen species via redox balance modulation. PMID: 26728324
    22. ANG II-induced IL-6 elevation in macrophages enhances activation of the JAK-STAT pathway and consequent angiotensinogen upregulation in proximal tubule cells, suggesting involvement of an immune response in driving intrarenal renin-angiotensin system activity PMID: 27009340
    23. intracellular ANG II stimulates AGT expression in the proximal tubules, leading to increased AGT formation and secretion into the tubular fluid, which contributes to ANG II-dependent hypertension PMID: 26864937
    24. Findings highlight the complexity of angiotensinogen (AGT)regulation and show that AGT protein and mRNA levels are responsive to angiotensin II in both SHR and Wistar astrocytes. Most importantly, our findings suggest that this peptide can induce its own synthesis by positively regulating AGT protein synthesis. PMID: 27131988
    25. CTRP6 improves PPARgamma activation and alleviates AngII-induced hypertension and vascular endothelial dysfunction PMID: 27871858
    26. H2S downregulates Ang II-induced atrial Kv1.5 expression by attenuating Nox4-related ROS-triggered P-Smad2/3 and P-ERK 1/2 activation during atrial fibrillation PMID: 28011270
    27. Compared to chymase, ACE-mediated angiotensin-(1-12) metabolism in the heart was several fold lower. Overall our findings suggest that angiotensin-(1-12), not Ang I, is the better substrate for Ang II formation by chymase in adult rats. PMID: 27465904
    28. glomerular expression of Renin-angiotensin system components in nephrotic models, was examined. PMID: 27932705
    29. These observations demonstrate that the effect of Ang-(1-12) on potassium current was related to the formation of Ang II and that the peptide has arrhythmogenic properties. PMID: 27590241
    30. enhancing Ang-(1-7)-Mas-R-nNOS system is likely to be beneficial in preventing against cardiovascular and cerebrovascular dysfunction. PMID: 27960152
    31. overproduction of O2 (-) through overexpression of CuZnSOD in the organum vasculosum of the lamina terminalis plays an important role in the development of chronic AngII-dependent hypertension PMID: 26881025
    32. The results of this study suggested that disruption of astroglial AOGEN synthesis leads to cardiovascular, cognitive, behavioral, and sleep disorders that might be partly due to LC dysfunction. PMID: 26775713
    33. A reciprocal regulatory relationship between DDR2 and collagen, involving cross-talk between the GPCR and RTK pathways, is central to Angiotensin II-induced increase in collagen expression in cardiac fibroblasts. PMID: 26674152
    34. Urinary AGT elevation prior to urinary protein levels suggests that AGT is an early biomarker of kidney dysfunction in intrauterine growth restricted offspring rats. PMID: 26270574
    35. The antiaging protein klotho inhibits angiotensin II -induced cardiomyocyte hypertrophy. PMID: 26970306
    36. PAR1 and PAR2 play a critical role in Ang II-induced adventitial fibroblasts activation, and this may contribute to adventitia-related pathological changes. PMID: 27012211
    37. Angiotensin II modifies carotid body chemosensitivity, suggest that the increased circulating levels of ANG II and leptin induced by intermittent hypoxia act at the carotid body to alter leptin signaling within the carotid body which in turn may influence chemoreceptor function. PMID: 26431621
    38. Data suggest that valsartan may decrease the secretion of transforming growth factor (TGF-beta) and fibronectin (FN) in a dose-dependent manner via inhibiting angiotensin II-notch1 receptor (AngII-Notch) signaling. PMID: 26728369
    39. Ang II promotes activation of ARF6 to controls reactive oxygen species production by regulating Rac1 activation and Nox1 expression. PMID: 26824355
    40. PGC-1-alpha inhibits AngII-induced vascular smooth muscle cells proliferation and migration. PMID: 26310573
    41. the augmented AGT released from apoptotic endothelial cells acts as a vital progenitor of Ang II to accelerate vascular remodelling PMID: 26147666
    42. miR-7a/b has an anti-fibrotic role in ANG II-treated CFs that is mediated by Sp1 mechanism involving the TGF-beta and MAPKs pathways. PMID: 25923922
    43. Compared to LIT, HIIT resulted in reduced Agt mRNA expression (p = 0.035), reduced plasma LDL (p = 0.001), triglycerides (p = 0.029), and total cholesterol (p = 0.002), increased plasma albumin (p = 0.047), reduced remnant kidney weight (p = 0.005) PMID: 26090382
    44. the effect of leptin treatment for 7 and 28 days on renal function and morphology and the participation of angiotensin II (Ang II), through its AT1 receptor, was examined. PMID: 25793389
    45. Ang II plays a role in Venezuelan equine encephalitis and that brain inflammation is protective against viral infection. PMID: 26156105
    46. Overexpression of clusterin in proximal tubular epithelial cells decreased the levels of Ang II-stimulated fibrotic markers and AT1R. PMID: 25148511
    47. These results suggest that Ang-(1-7) increases DUSP1 to reduce MAP kinase/Smad/CTGF signaling and decrease fibrosis in resistance arterioles, to attenuate end-organ damage associated with chronic hypertension. PMID: 25079175
    48. In diabetic rats, renal angiotensinogen is positively associated with diabetic albuminuria and contributes to the sex-dependent differences in renal injury. PMID: 25529203
    49. findings suggest that an increased concentrations of Ang peptides in the systemic circulation during exercise may promote neurogenesis in the adult rat hippocampus PMID: 25223907
    50. Ang-(1-7) abolished advanced glycated end product-induced activation of the MAP kinase ERK1/2. PMID: 25246357

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  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Serpin family
  • 组织特异性：
    Expressed by the liver and secreted in plasma.
  • 数据库链接：

    KEGG: rno:24179

    STRING: 10116.ENSRNOP00000024917

    UniGene: Rn.6319