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  4. Recombinant Rat Alpha-crystallin A chain (Cryaa)

Recombinant Rat Alpha-crystallin A chain (Cryaa)

  • 货号：
    CSB-YP006007RA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP006007RA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP006007RA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP006007RA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP006007RA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Cryaa
  • Uniprot No.：
  • 别名：
    Cryaa; Crya1Alpha-crystallin A chain [Cleaved into: Alpha-crystallin A(1-168); Alpha-crystallin A(1-165); Alpha-crystallin A(1-163); Alpha-crystallin A(1-162); Alpha-crystallin A(1-157); Alpha-crystallin A(1-156); Alpha-crystallin A(1-151)]
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-196
  • 氨基酸序列
    MDVTIQHPWF KRALGPFYPS RLFDQFFGEG LFEYDLLPFL SSTISPYYRQ SLFRTVLDSG ISELMTHMWF VMHQPHAGNP KNNPGKVRSD RDKFVIFLDV KHFSPEDLTV KVLEDFVEIH GKHNERQDDH GYISREFHRR YRLPSNVDQS ALSCSLSADG MLTFSGPKVQ SGLDAGHSER AIPVSREEKP SSAPSS
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Contributes to the transparency and refractive index of the lens. Acts as a chaperone, preventing aggregation of various proteins under a wide range of stress conditions. Required for the correct formation of lens intermediate filaments as part of a complex composed of BFSP1, BFSP2 and CRYAA.; Inhibits bacterial growth in the lens.
  • 基因功能参考文献：
    1. Rat crystallin alpha A a encode activated chaperones. PMID: 26378715
    2. Cryaa is a moonlighting protein that functions as a heat shock protein as well as a lens crystalline. PMID: 7556464
    3. The alphaA- and alphaB-crystallin peptides inhibited stress-induced aggregation of four client proteins, and the alphaA-acetyl peptide was more effective than the native peptide against three of the client proteins. PMID: 23508955
    4. alphaA-crystallin and alphaB-crystallin reside in separate subcellular compartments in the developing ocular lens PMID: 23071119
    5. The main differences, which could be attributed to the cataract-specific processes, are the faster decay of the content of gamma-crystallins and the significant decrease of unmodified alphaA-crystallin abundance in the senescent rat lenses. PMID: 21677790
    6. Curcumin suppressed the expression of selenite-induced alphaA- and alphaB-crystallin and Hsp 70, and may therefore suppress cataract formation in rat pups PMID: 21311744
    7. hydroxylation and subsequent phosphorylation of a proline residue in alpha-crystallin A in the eye as well as in heart tissue of rat. PMID: 20682783
    8. An important role is demonstrated for the C-terminal extension of alpha A crystallin, Arg-163 in particular, whereas no significant role is found for the C-terminal flexible tail in the oligomer assembly of alpha A-crystallin. PMID: 14529298
    9. In samples from rats of all ages, the content of alpha A crystallin is significantly decreased in the oldest rats, indicative that protection against stress-induced protein aggregation is compromised in the aged retina. PMID: 14690441
    10. The results indicate that elevated expression of alpha-crystallins in some tissues may have implications in pathophysiology of diabetic complications. PMID: 16309625
    11. The role of arginine-163 is to provide a positive charge for intersubunit electrostatic interactions in the carboxy-terminal domain of alpha A-crystallin. PMID: 17176090
    12. alpha A-K11T and alpha B-K90T/K92T mutants showed the largest decrease in glycation and increase in chaperone activity. PMID: 18158587
    13. Enhanced survival of axotomized axons was observed beyond the crush site after a single intravitreal administration of alpha-crystallin at the time of axotomy. PMID: 18551258

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  • 亚细胞定位：
    Cytoplasm. Nucleus.
  • 蛋白家族：
    Small heat shock protein (HSP20) family
  • 组织特异性：
    Highly expressed in eye lens. Also expressed in non-lenticular tissues such as brain, spleen, liver, lung, skin, small intestine and a several epithelial and fibroblast cell lines with highest levels in spleen.
  • 数据库链接：

    KEGG: rno:24273

    STRING: 10116.ENSRNOP00000065714

    UniGene: Rn.127769