Your Good Partner in Biology Research

  1. 首页
  2. 蛋白
  3. 重组蛋白
  4. Recombinant Rat 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3 (Pfkfb3)

Recombinant Rat 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3 (Pfkfb3)

  • 中文名称：
    大鼠Pfkfb3重组蛋白
  • 货号：
    CSB-YP017819RA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    大鼠Pfkfb3重组蛋白
  • 货号：
    CSB-EP017819RA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    大鼠Pfkfb3重组蛋白
  • 货号：
    CSB-EP017819RA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    大鼠Pfkfb3重组蛋白
  • 货号：
    CSB-BP017819RA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    大鼠Pfkfb3重组蛋白
  • 货号：
    CSB-MP017819RA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Pfkfb36-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3; 6PF-2-K/Fru-2,6-P2ase 3; PFK/FBPase 3; 6PF-2-K/Fru-2,6-P2ase brain-type isozyme; RB2K) [Includes: 6-phosphofructo-2-kinase; EC 2.7.1.105); Fructose-2,6-bisphosphatase; EC 3.1.3.46)]
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-555
  • 氨基酸序列
    MPLELTQSRV QKIWVPVDHR PSLPRSCGPK LTNSPTVIVM VGLPARGKTY ISKKLTRYLN WIGVPTKVFN VGEYRREAVK QYSSYNFFRP DNEEAMRVRK QCALAALRDV KSYLTKEGGQ IAVFDATNTT RERRHMILHF AKENDFKAFF IESVCDDPTV VASNIMEVKI SSPDYKDCNS AEAMDDFMKR INCYEASYQP LDPDKCDRDL SFIKVIDVGR RFLVNRVQDH IQSRIVYYLM NIHVQPRTIY LCRHGENEYN VQGKIGGDSG LSSRGKKFAN ALSKFVEEQN LKDLRVWTSQ LKSTIQTAEA LRLPYEQWKA LNEIDAGVCE ELTYEEIRDT YPEEYALREQ DKYYYRYPTG ESYQDLVQRL EPVIMELERQ ENVLVICHQA VLRCLLAYFL DKSAEEMPYL KCPLHTVLKL TPVAYGCRVE SIYLNVESVS THRERSEAVK IQHFASVVRP SSYTELDFLS VESAKQDAKK GPNPLMRRNS VTPLASPEPT KKPRINSFEE HVASTSAALP SCLPPEVPTQ LPGQPLLGKA CLRTVCHIFS KFSPY
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Synthesis and degradation of fructose 2,6-bisphosphate.
  • 基因功能参考文献：
    1. Increased PFKFB3 expression, but not increased PFKFB3 Ser461 phosphorylation, plays a role in increasing glycolysis in mitogen-stimulated thymocytes. PMID: 28235572
    2. The PFK2 expression along with LDH-4 were observed to be increased ~2-fold (P < 0.001) in 0.5 mM ammonia treated brain slices. PMID: 23703029
    3. The expression of PFKFB3 lacking the APC(Cdh1)-targeting KEN motif, promoted accumulation of PFKFB3 and its release from the nucleus to the cytosol through an excess Cdh1-inhibitable process. PMID: 22421967
    4. Overexpression of PFK2 potentiated glucokinase expression in hepatocytes transduced with an adenoviral vector-encoding glucokinase. PMID: 15983194
    5. Here, we characterize a new kidney- and liver-specific Pfkfb3 isozyme, a product of the RB2K3 splice variant, and demonstrate that its expression, in primary cultured hepatocytes, depends on hepatic cell proliferation and dedifferentiation. PMID: 18191036
    6. Results show that Pfkfb3 is absent from neurons in the brain cortex and that Pfkfb3 in neurons is constantly subject to proteasomal degradation by the action of the E3 ubiquitin ligase, anaphase-promoting complex/cyclosome (APC/C)-Cdh1. PMID: 19448625

    显示更多

    收起更多

  • 蛋白家族：
    Phosphoglycerate mutase family
  • 数据库链接：

    KEGG: rno:117276

    STRING: 10116.ENSRNOP00000062965

    UniGene: Rn.10791