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  4. Recombinant Ras protein let-60 (let-60)

Recombinant Ras protein let-60 (let-60)

  • 货号：
    CSB-YP340505CXY
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP340505CXY
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP340505CXY-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP340505CXY
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP340505CXY
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    let-60
  • Uniprot No.：
  • 别名：
    let-60; lin-34; ZK792.6Ras protein let-60; Abnormal cell lineage protein 34; Lethal protein 60
  • 种属：
    Caenorhabditis elegans
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-181
  • 氨基酸序列
    MTEYKLVVVG DGGVGKSALT IQLIQNHFVE EYDPTIEDSY RKQVVIDGET CLLDILDTAG QEEYSAMRDQ YMRTGEGFLL VFAVNEAKSF ENVANYREQI RRVKDSDDVP MVLVGNKCDL SSRSVDFRTV SETAKGYGIP NVDTSAKTRM GVDEAFYTLV REIRKHRERH DNNKPQKKKK C
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    The level of let-60 controls the switch between vulval and hypodermal cell fates during C.elegans vulval induction. May stimulate the guanine nucleotide exchange factor (GEF) activity of rap-1. May induce nuclear condensation.
  • 基因功能参考文献：
    1. Expression of activated LET-60/Ras in the neighboring duct cell, but not in the G1 or G2 cells, is sufficient to rescue sos-1 delamination defects, revealing that Ras acts non-cell-autonomously to permit G1 delamination. PMID: 25371363
    2. Canal cell asymmetry and let-60/Ras signaling influence which of two equivalent precursors will attach to the canal cell. PMID: 21771815
    3. LET-60 (Ras) is identified as a critical RGEF-1b substrate-effector in sensory neurons. PMID: 21482356
    4. LET-60 Ras GTPase might act with FLI-1 to control germ line morphogenesis PMID: 18485202
    5. Goa-1 Goalpha and let-60 Ras act in parallel to gpc-1 in olfactory pathway. PMID: 19189947

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  • 亚细胞定位：
    Cell membrane; Lipid-anchor.
  • 蛋白家族：
    Small GTPase superfamily, Ras family
  • 组织特异性：
    Expressed in body wall muscles and in the nervous system including ganglion, nerve ring dorsal and ventral nerve cords, motor neurons and sensory tail neurons.
  • 数据库链接：

    KEGG: cel:CELE_ZK792.6

    STRING: 6239.ZK792.6

    UniGene: Cel.19758