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  4. Recombinant Probable ribonuclease VapC20 (vapC20)

Recombinant Probable ribonuclease VapC20 (vapC20)

  • 货号：
    CSB-YP308684MVZ
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP308684MVZ
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP308684MVZ-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP308684MVZ
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP308684MVZ
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    vapC20
  • Uniprot No.：
  • 别名：
    vapC20; Rv2549c; 23S rRNA-specific endonuclease VapC20; EC 3.1.-.-; Ribonuclease VapC20; RNase VapC20; Toxin VapC20
  • 种属：
    Mycobacterium tuberculosis
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-131
  • 氨基酸序列
    MIFVDTSFWA ALGNAGDARH GTAKRLWASK PPVVMTSNHV LGETWTLLNR RCGHRAAVAA AAIRLSTVVR VEHVTADLEE QAWEWLVRHD EREYSFVDAT SFAVMRKKGI QNAYAFDGDF SAAGFVEVRP E
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Toxic component of a type II toxin-antitoxin (TA) system. An endoribonuclease that cleaves both E.coli and M.smegmatis 23S rRNA between G2661 and A2662 in the sarcin-ricin loop (SRL, E.coli 23S rRNA numbering). The SRL sequence is highly conserved and is implicated in GTP hydrolysis by EF-Tu and EF-G. Acts on purified ribosomes but not on isolated RNA in E.coli, nor on a shortened artificial substrate. Upon expression in E.coli inhibits cell growth, colony formation and translation. Its toxic effect is neutralized by coexpression, or subsequent expression (tested over 2 hours) with cognate antitoxin VapB20.
  • 基因功能参考文献：
    1. Data show that VapB20 exists as a homodimer in solution and ssociates with VapC20 dimers to form heterotetramers and heterooctamers in a concentration-dependent manner. PMID: 28986943
  • 蛋白家族：
    PINc/VapC protein family
  • 数据库链接：

    KEGG: mtu:Rv2549c

    STRING: 83332.Rv2549c