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  4. Recombinant Pig Interferon gamma (IFNG)

Recombinant Pig Interferon gamma (IFNG)

  • 货号：
    CSB-YP011050PI
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP011050PI
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP011050PI-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP011050PI
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP011050PI
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    IFNGInterferon gamma; IFN-gamma
  • 种属：
    Sus scrofa (Pig)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    24-166
  • 氨基酸序列
    QAPFFKE ITILKDYFNA STSDVPNGGP LFLEILKNWK EESDKKIIQS QIVSFYFKFF EIFKDNQAIQ RSMDVIKQDM FQRFLNGSSG KLNDFEKLIK IPVDNLQIQR KAISELIKVM NDLSPRSNLR KRKRSQTMFQ GQRASK
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Type II interferon produced by immune cells such as T-cells and NK cells that plays crucial roles in antimicrobial, antiviral, and antitumor responses by activating effector immune cells and enhancing antigen presentation. Primarily signals through the JAK-STAT pathway after interaction with its receptor IFNGR1 to affect gene regulation. Upon IFNG binding, IFNGR1 intracellular domain opens out to allow association of downstream signaling components JAK2, JAK1 and STAT1, leading to STAT1 activation, nuclear translocation and transcription of IFNG-regulated genes. Many of the induced genes are transcription factors such as IRF1 that are able to further drive regulation of a next wave of transcription. Plays a role in class I antigen presentation pathway by inducing a replacement of catalytic proteasome subunits with immunoproteasome subunits. In turn, increases the quantity, quality, and repertoire of peptides for class I MHC loading. Increases the efficiency of peptide generation also by inducing the expression of activator PA28 that associates with the proteasome and alters its proteolytic cleavage preference. Up-regulates as well MHC II complexes on the cell surface by promoting expression of several key molecules such as cathepsins B/CTSB, H/CTSH, and L/CTSL. Participates in the regulation of hematopoietic stem cells during development and under homeostatic conditions by affecting their development, quiescence, and differentiation.
  • 基因功能参考文献：
    1. The regulatory effect of IFN-gamma on CYP3A29 expression is mediated via PXR. PMID: 25413352
    2. Mapping of QTL for mycoplasma and tetanus antibodies and IFNgamma. PMID: 20567833
    3. Data show taht the expression of PoIFN-gamma in insect cells was confirmed by Western Blot, indirect immunofluorescence assay and indirect ELISA. PMID: 17577980
    4. IFNgamma production from the frozen peripheral blood mononuclear cells was significantly higher than that from the fresh ones. PMID: 19766617
    5. Translational control of IL-18 expression by its 5'-UTR limits production of IL-18, resulting in restricted expression of mRNA and protein IFN-gamma in this model of crescentic glomerulonephritis(GN). Might amplify CD8+-mediated macrophage-dependent GN. PMID: 12787406
    6. selective changes in immature crypt cells induced by IFN-gamma bound to extracellular matrix could contribute to inappropriate responsiveness to commensal bacteria in inflammatory bowel diseases PMID: 15894119
    7. A functional single nucleotide polymorphism is reported in the coding region of IFN-gamma cDNA that markedly reduces antiviral activity of the IFN-gamma protein. PMID: 17416419
    8. The expression of IFN-gamma in recombiannt Lactococcus lactis as a tool for investigating downregulation of allergic predisposition of pigs to experimental food allergy is reported. PMID: 18164876

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  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Type II (or gamma) interferon family
  • 组织特异性：
    Released primarily from activated T lymphocytes.
  • 数据库链接：

    KEGG: ssc:396991

    STRING: 9823.ENSSSCP00000000511

    UniGene: Ssc.4093