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  4. Recombinant Pharynx and intestine in excess protein 1 (pie-1)

Recombinant Pharynx and intestine in excess protein 1 (pie-1)

  • 中文名称：
    秀丽隐杆线虫pie-1重组蛋白
  • 货号：
    CSB-YP853181CXY
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    秀丽隐杆线虫pie-1重组蛋白
  • 货号：
    CSB-EP853181CXY
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    秀丽隐杆线虫pie-1重组蛋白
  • 货号：
    CSB-EP853181CXY-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    秀丽隐杆线虫pie-1重组蛋白
  • 货号：
    CSB-BP853181CXY
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    秀丽隐杆线虫pie-1重组蛋白
  • 货号：
    CSB-MP853181CXY
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    pie-1
  • Uniprot No.：
  • 别名：
    pie-1; Y49E10.14; Pharynx and intestine in excess protein 1; Protein pie-1
  • 种属：
    Caenorhabditis elegans
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-335
  • 氨基酸序列
    MAQTKPIAEQ MAALNNSDDT SFAADRSNSL LNATCPARIQ NSVDQRKINR SFNDSLSSGY SGKWLRPKRE ALKITPLAQI DEAPATKRHS SAKDKHTEYK TRLCDAFRRE GYCPYNDNCT YAHGQDELRV PRRRQEYYSR DPPRERRDSR SRRDDVDTTI NRSSSSASKH HDENRRPSNN HGSSNRRQIC HNFERGNCRY GPRCRFIHVE QMQHFNANAT VYAPPSSDCP PPIAYYHHHP QHQQQFLPFP MPYFLAPPPQ AQQGAPFPVQ YIPQQHDLMN SQPMYAPMAP TYYYQPINSN GMPMMDVTID PNATGGAFEV FPDGFFSQPP PTIIS
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Maternally provided PIE-1 is required for germline cell fate determination. Functions as a repressor of RNA polymerase II-dependent gene expression in the developing germline. Required for expression of nos-2 in P4 germline blastomere cells. Inhibits the histone deacetylase activity of hda-1. Represses transcriptional activation of cdk-9 and cit-1.1, which are members of the P-TEFb complex.
  • 基因功能参考文献：
    1. Degradation of this maternally supplied cell fate determinant is delayed in oma-1 (zu405) mutant embryos. PMID: 12781695
    2. These results suggest that PIE-1 uses partially redundant mechanisms to block transcription by targeting both the initiation and elongation phases of the transcription cycle. PMID: 18202370
    3. Results support a heterogeneous reaction/diffusion model for PIE-1 enrichment in which the diffusion coefficient of PIE-1 is reversibly reduced in the posterior, resulting in a stable protein gradient across the zygote at steady state. PMID: 19221192
  • 亚细胞定位：
    Nucleus. Cytoplasm. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle. Cytoplasmic granule. Note=Initially associates with both centrosomes of the mitotic spindle. Rapidly disappears from the centrosome destined for the somatic daughter and persists in the centrosome of the daughter that becomes the next germline blastomere. Accumulates in larger cytoplasmic granules (P granules), which are visible around the nuclei in the micrographs.
  • 数据库链接：

    KEGG: cel:CELE_Y49E10.14

    STRING: 6239.Y49E10.14a.2

    UniGene: Cel.19704