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  4. Recombinant Multifunctional cyclase-dehydratase-3-O-methyl transferase tcmN (tcmN)

Recombinant Multifunctional cyclase-dehydratase-3-O-methyl transferase tcmN (tcmN)

  • 中文名称：
    Recombinant Multifunctional cyclase-dehydratase-3-O-methyl transferase tcmN(tcmN),Yeast
  • 货号：
    CSB-YP324550SOG
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    Recombinant Multifunctional cyclase-dehydratase-3-O-methyl transferase tcmN(tcmN),Yeast
  • 货号：
    CSB-EP324550SOG
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    Recombinant Multifunctional cyclase-dehydratase-3-O-methyl transferase tcmN(tcmN),Yeast
  • 货号：
    CSB-EP324550SOG-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    Recombinant Multifunctional cyclase-dehydratase-3-O-methyl transferase tcmN(tcmN),Yeast
  • 货号：
    CSB-BP324550SOG
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    Recombinant Multifunctional cyclase-dehydratase-3-O-methyl transferase tcmN(tcmN),Yeast
  • 货号：
    CSB-MP324550SOG
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    tcmN
  • Uniprot No.：
  • 别名：
    tcmN; Multifunctional cyclase-dehydratase-3-O-methyl transferase TcmN; EC 2.1.1.-
  • 种属：
    Streptomyces glaucescens
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-494
  • 氨基酸序列
    MAARTDNSIV VNAPFELVWD VTNDIEAWPE LFSEYAEAEI LRQDGDGFDF RLKTRPDANG RVWEWVSHRV PDKGSRTVRA HRVETGPFAY MNLHWTYRAV AGGTEMRWVQ EFDMKPGAPF DNAHMTAHLN TTTRANMERI KKIIEDRHRE GQRTPASVLP TELHAQQLLL LAASGRLARI VHVLTELRIA DLLADGPRHV AELAKETDTH ELSLYRVLRS AASVGVFAEG PVRTFSATPL SDGLRTGNPD GVLPLVKYNN MELTRRPYDE IMHSVRTGEP AFRRVFGSSF FEHLEANPEA GEFFERFMAH WSRRLVLDGL ADQGMERFSR IADLGGGDGW FLAQILRRHP HATGLLMDLP RVAASAGPVL EEAKVADRVT VLPGDFFTDP VPTGYDAYLF KGVLHNWSDE RAVTVLRRVR EAIGDDDARL LIFDQVMAPE NEWDHAKLLD IDMLVLFGGR ERVLAEWRQL LLEADFDIVN TPSHTWTTLE CRPV
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    The N-terminal domain enhances the formation of an early, partially cyclized intermediate, while the C-terminal domain catalyzes the 3-O-methylation of one or more later intermediates in the biosynthetic pathway. Catalyzes the methylation of tetracenomycin D3 (Tcm D3) to yield Tcm B3. Catalyzes as well the following side reactions: methylation of 8-O-methyl-Tcm D3 to yield Tcm E; and of 9-carboxymethyl-Tcm B3 to yield Tcm A2.
  • 蛋白家族：
    Class I-like SAM-binding methyltransferase superfamily, Cation-independent O-methyltransferase family