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  4. Recombinant Mouse cAMP-responsive element modulator (Crem)

Recombinant Mouse cAMP-responsive element modulator (Crem)

  • 货号：
    CSB-YP005961MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP005961MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP005961MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP005961MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP005961MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Crem
  • Uniprot No.：
  • 别名：
    CremcAMP-responsive element modulator; Inducible cAMP early repressor; ICER
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-357
  • 氨基酸序列
    MSKCGRKKYM RTNVRQMTME TVESQQDRSV TRSVAEHSSA HMQTGQISVP TLAQVSVAGS GTGRGSPAVT LVQLPSGQTV QVQGVIQTPH PSVIQSPQIQ TVQVATIAET DDSADSEVID SHKRREILSR RPSYRKILNE LSSDVPGIPK IEEEKSEEEG TPPNIATMAV PTSIYQTSTG QYIAIAQGGT IQISNPGSDG VQGLQALTMT NSGAPPPGAT IVQYAAQSAD GTQQFFVPGS QVVVQDEETD LAPSHMAAAT GDMPTYQIRA PTTALPQGVV MAASPGSLHS PQQLAEEATR KRELRLMKNR EAAKECRRRK KEYVKCLESR VAVLEVQNKK LIEELETLKD ICSPKTD
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Transcriptional regulator that binds the cAMP response element (CRE), a sequence present in many viral and cellular promoters. Isoforms are either transcriptional activators or repressors. Isoform 2, isoform 3 and isoform 4 are repressors, while isoform 1 is an activator. Plays a role in spermatogenesis and is involved in spermatid maturation. Binding of isoform 1 (activator) to CRE is increased by CREB3L4. The CREM isoform 1-CREB3L4 heterodimer functions through CRE and may recruit HIRA to CRE to regulate histone exchange.; Plays a role in the regulation of the circadian clock: acts as a transcriptional repressor of the core circadian component PER1 by directly binding to cAMP response elements in its promoter.
  • 基因功能参考文献：
    1. Studied role and importance of inducible cAMP early repressor (ICER) in glutaminolysis and the generation of Th17 cells. Demonstrated the IL-17 promoting ICER works through the direct control of glutaminase 1 (Gls1) by binding its promoter. PMID: 29463741
    2. Deficiency in ICER elevated binding of NF-kappaB to promoters of pro-inflammatory genes and their subsequent gene expression. Mice deficient in ICER were hypersensitive to LPS-induced endotoxic shock and showed propagated inflammation. PMID: 28009352
    3. The transcription factor CREM was found to be important in the regulation of cardiotoxicity-associated genes. PMID: 28368536
    4. Our results demonstrate that overexpression of CREMalpha in T cells changes the inflammatory milieu, attenuating initiation and progression of CLD. PMID: 27686093
    5. the role of ICER in ovarian function, was investigated. PMID: 27590584
    6. Phosphorylated Tssk4 might participate in testis genes expressions by phosphorylating Crem at Ser-117. PMID: 26940607
    7. the signaling pathways mediating GnRH activation of CREB and ICER are distinct, contributing to the decoding of the pulsatile GnRH to regulate FSHbeta expression. PMID: 26835742
    8. Crem(-/-) mice exhibited increased proliferation of primary aortic VSMCs along with upregulation of PDGF signaling. PMID: 25425331
    9. overexpressed in the nuclei of hepatocellular carcinoma cells PMID: 25401338
    10. Unlike in T cells from spleen, CREMalpha overexpression did not induce a predominant Th17 response in intrahepatic T cells PMID: 25330465
    11. These data identify ICER as a redundant mediator of Treg cells and cAMP action on Teff cells and suggest that Epac may function as an alternative effector to promote cAMP-dependent Teff cell suppression. PMID: 24007532
    12. The results of this study suggested that activation of CREMtau may provide a means to accelerate the therapeutic efficacy of current antidepressant treatment. PMID: 23966689
    13. levels of CREM in T cells determine the outcome of acute lung injury, and CREMalpha transgenic animals represent a model in which proinflammatory T cells aggravate acute lung injury in different phases of the disease. PMID: 23785120
    14. Reduction of connexin36 content by ICER-1 contributes to insulin-secreting cells apoptosis induced by oxidized LDL particles. PMID: 23383107
    15. Inducible cAMP early repressor regulates the Period 1 gene of the hepatic and adrenal clocks. PMID: 23443664
    16. Data suggest that expression of TRH (thyrotropin-releasing hormone) is up-regulated by CREM in hypothalamus; however, regulation of TRH by triiodothyronine appears independent of expression of CREM or CREB (cAMP responsive element binding protein). PMID: 23000398
    17. analysis of insights into the downstream pathways and targets controlled by transcription factors CREM in the testis PMID: 22384077
    18. CREMalpha overexpression decreases IL-2 production, induces a T(H)17 phenotype and accelerates autoimmunity. PMID: 22355096
    19. nTregs suppress T-cell responses by the cAMP-dependent nuclear accumulation of ICER/CREM and inhibition of NFATc1 and IL-2 induction. PMID: 21262800
    20. PDE8 as a novel target for suppression of Teff cell functions, including adhesion to endothelial cells PMID: 20711499
    21. Results indicate that SPAG8 acts as a regulator of ACT and plays an important role in CREM-ACT-mediated gene transcription during spermatogenesis. PMID: 20488182
    22. Data suggest that CGRP causes rapid up-regulation of ICER, which in turn competes with ATF-2 for binding to the Tnfa promoter, leading to repression of gene expression. PMID: 20018859
    23. the Jmjd1a-controlled epigenetic histone modifications are crucial for Crem-regulated gene expression and spermatogenesis PMID: 19910458
    24. suggest that ICER production antagonizes the stimulatory action of CRE binding protein to attenuate follicle-stimulating hormone beta transcription at high gonadotropin-releasing hormone pulse frequencies. PMID: 20008557
    25. Results demonstrate the essential role of CREM in spermatogenesis and are reminiscent of some cases of male infertility. (review) PMID: 9159233
    26. according to findings, germ cell differentiation is dependent on cAMP response element modulator function PMID: 12297517
    27. Impaired cardiac contraction and relaxation and decreased expression of sarcoplasmic Ca2+-ATPase in mice lacking the CREM gene. PMID: 12475904
    28. Results demonstrate novel involvement of CREM in regulation of protein phosphatase-1 activity and of phospholamban, likely resulting in a potent frequency-dependent influence on cardiac function. PMID: 12554693
    29. CREM is stimulated in macrophages by interferon gamma through the activation of casein kinase 2 PMID: 12609974
    30. crem expression in osteoblasts requires activation of the cAMP-PKA signaling pathway. PMID: 12753864
    31. CREM interacts with transcription factor IIA (TFIIA), a general transcription factor that stimulates RNA polymerase II-directed transcription PMID: 14512522
    32. Data characterize the genes that are controlled by CREM during post-meiotic differentiation of round spermatids. PMID: 14654248
    33. TB-RBP-mouse ribonucleoprotein complex transports a group of specific CREM-regulated mRNAs in mammalian male PMID: 14673085
    34. Alternative transcription of the cAMP response element-modulator (CREM) gene yields ICER which is required for desipramine to reduce stress-induced corticosterone release through regulation of hypothalamic CRF expression PMID: 14985439
    35. Distinct distribution of ICER- immunoreactivity throughout the mouse brain and pituitary forms the basis to better understand the mandatory role of this inhibitory transcription factor within cyclic AMP signaling. PMID: 15384069
    36. Tisp40 and CREM bind to the Tisp40 promoter in vivo PMID: 16595651
    37. We review emerging, novel mechanisms involved in contact-dependent, TR-mediated suppression of IL-2 production in responder CD25- T lymphocytes and the potential involvement of inducible cAMP early repressor (ICER) in this suppression. PMID: 17028200
    38. Icer/Crem is a dominant repressor of contact-dependent T-regulatory cell-mediated suppression that effects transcriptional attenuation of IL-2 production in CD25+ Foxp3-negative effector T cells. PMID: 17372992
    39. Activation of calcitonin gene-related peptide (CGRP) in dendritic cells upregulates the expression of cAMP-inducible transcriptional repressor ICER, which in turn dampens gene transcription. PMID: 17579082
    40. the gender difference in the severity of diabetes and diabetic nephropathy was evident with age in ICER Igamma transgenic diabetic mouse PMID: 17690470
    41. knockdown of CREMalpha or DUSP14 or expression of a dominant-negative form of DUSP14 increased beta-cell line proliferation and enhanced the GLP-1-induced proliferation of primary beta-cells PMID: 18025410
    42. chronic exposure to palmitate inhibits the Cx36 expression through PKA-mediated ICER-1gamma overexpression PMID: 18073214
    43. Together these data suggest that the increase in ICER mRNA following SE may have a role in suppressing the severity of epilepsy. PMID: 18295410
    44. ICER regulates the activity and/or expression of ATF/CREB factors required for normal bone formation. PMID: 18460422
    45. Inducible cAMP early response element (ICER) negatively regulates the neuronal processes required for long-term fear memory and neuronal plasticity. PMID: 18562617
    46. For the first time there was shown that isoforms of Crem regulate the circadian expression of Cyp51 and other cholesterogenic genes in the mouse liver. PMID: 18775413
    47. the regulation of genes by CREM as an important mechanism of beta(1)AR-induced cardiac damage in mice PMID: 19103994
    48. CREMalpha is an important negative regulator of costimulation and APC-dependent T cell function PMID: 19299714

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  • 亚细胞定位：
    Nucleus.; [Isoform 11]: Cytoplasm. Nucleus.
  • 蛋白家族：
    BZIP family
  • 组织特异性：
    Expressed in the testis.
  • 数据库链接：

    KEGG: mmu:12916

    STRING: 10090.ENSMUSP00000121233

    UniGene: Mm.5244