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  4. Recombinant Mouse Zinc finger protein 57 (Zfp57)

Recombinant Mouse Zinc finger protein 57 (Zfp57)

  • 货号：
    CSB-YP026851MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP026851MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP026851MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP026851MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP026851MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Zfp57
  • Uniprot No.：
  • 别名：
    Zfp57Zinc finger protein 57; Zfp-57
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-421
  • 氨基酸序列
    MAARKQSSQP SRTPVSYEDV AVSFTQEEWE YLTSTQKTLY QKVMSETFKN LTFVGSKKKP QEPSSDLQDK NEEQEKSSSC TGVFKGGPFF FCLTCGKCFK KNTFLFNHQF PVRSRRLAVT NPQSRKGKGY KAQHRGERPF FCNFCGKTYR DASGLSRHRR AHLGYRPRSC PECGKCFRDQ SEVNRHLKVH QNKPAASNQA GNQASNQRLK SRVPPTTPRS QAPALKYVKV IQGPVARAKA RNSGASTLNV RSNSITVVRS REKISCPYCH ITFTMRTCLL THLKIHFRRQ PNQHFCCKES AHSSNTLRMQ KIYTCPVCDS SFRGKESLLD HLCCQRPIRF SKCWEILGHL LGYLHEPVVL GNIFKVRDSS GKRMESRRRR RKRACTENPE TEGLSGKGRV APWEMEGATS PESPVTEEDS D
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Transcription regulator required to maintain maternal and paternal gene imprinting, a process by which gene expression is restricted in a parent of origin-specific manner by epigenetic modification of genomic DNA and chromatin, including DNA methylation. Acts by controlling DNA methylation during the earliest multicellular stages of development at multiple imprinting control regions (ICRs). Acts together with ZNF445, but ZFP57 plays the predominant role in imprinting maintenance. In contrast, in humans, ZNF445 seems to be the major factor early embryonic imprinting maintenance. Required for the establishment of maternal methylation imprints at SNRPN locus. Acts as a transcriptional repressor in Schwann cells. Binds to a 5'-TGCCGC-3' consensus sequence and recognizes the methylated CpG within this element.
  • 基因功能参考文献：
    1. A group of functionally significant composite DNA elements (ZFP57 binding site (ZFBS)-Morph overlaps) that may play dual roles in the regulation of allele-specific gene expression. PMID: 28476430
    2. another function of ZFP57 in early embryogenesis is to repress cis-acting regulatory elements whose activity is not yet required. PMID: 27257070
    3. Data show that ZFP57 binding is affected by single nucleotide polymorphism (SNPs) and closely associated TGCCGC motif sequences that are present at the target sites. PMID: 26481358
    4. Within established mouse imprinted clusters, ZFP57 specifically binds known or predicted imprinting control region elements, but does not interact with secondary somatically differentially methylated regions (DMRs) or germline DMRs that are not imprinting controlling. PMID: 26025256
    5. ZFP57 may modulate NOTCH signaling during cardiac development. PMID: 25848000
    6. Taken together, our results suggest that Nanog positively regulates Zfp57 expression in multiple types of cells. PMID: 25445595
    7. Mouse and human ZFP57 are orthologs despite relatively low sequence identity. PMID: 24135613
    8. Data suggest that Glu182 in the DNA binding site in zinc finger motif of Zfp57 plays important role in binding specificity and affinity for 5-carboxylcytosine and other cytosine analogs. PMID: 24236546
    9. the structure of the DNA-binding domain of Zfp57, consisting of two adjacent zinc fingers, in complex with fully methylated DNA PMID: 23059534
    10. ZFP57 may recruit DNA methyltransferases to its target regions to maintain DNA methylation imprint, and this interaction is likely facilitated by KAP1/TRIM28/TIF1beta. PMID: 22144682
    11. Zpf57 maintains both maternal and paternal genomic imprints. PMID: 18854139

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  • 亚细胞定位：
    Nucleus. Note=Binds various differentially methylated regions (DMR), including the Snrpn DMR.
  • 蛋白家族：
    Krueppel C2H2-type zinc-finger protein family
  • 组织特异性：
    Expressed in oocytes and in a subset of adult tissues. Expressed at high levels in testis, and at low levels in cerebellum. Present in sciatic nerve and spinal cord (at protein level).
  • 数据库链接：

    KEGG: mmu:22715

    STRING: 10090.ENSMUSP00000065811

    UniGene: Mm.305561