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  4. Recombinant Mouse Zinc finger and BTB domain-containing protein 7A (Zbtb7a)

Recombinant Mouse Zinc finger and BTB domain-containing protein 7A (Zbtb7a)

  • 货号：
    CSB-YP026343MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP026343MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP026343MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP026343MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP026343MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Zbtb7a
  • Uniprot No.：
  • 别名：
    Zbtb7a; Lrf; Zbtb7; Zinc finger and BTB domain-containing protein 7A; Leukemia/lymphoma-related factor; POZ and Krueppel erythroid myeloid ontogenic factor; POK erythroid myeloid ontogenic factor; Pokemon
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-569
  • 氨基酸序列
    MAGGVDGPIG IPFPDHSSDI LSGLNEQRTQ GLLCDVVILV EGREFPTHRS VLAACSQYFK KLFTSGAVVD QQNVYEIDFV SAEALTALMD FAYTATLTVS TANVGDILSA ARLLEIPAVS HVCADLLERQ ILAADDVGDA SQPDGAGPTD QRNLLRAKEY LEFFRSNPMN SLPPTAFPWS GFGAPDDDLD ATKEAVAAAV AAVAAGDCNG LDFYGPGPPA DRPPAGDGDE GDSTPGLWPE RDEDAPPGGL FPPPTAPPAT TQNGHYGRAG AGTGEEEAAA LSEAAPEPGD SPGFLSGAAE GEDGDAADVD GLAASTLLQQ MMSSVGRAGD SDEESRTDDK GVMDYYLKYF SGAHEGDVYP AWSQKGEKKI RAKAFQKCPI CEKVIQGAGK LPRHIRTHTG EKPYECNICK VRFTRQDKLK VHMRKHTGEK PYLCQQCGAA FAHNYDLKNH MRVHTGLRPY QCDSCCKTFV RSDHLHRHLK KDGCNGVPSR RGRKPRVRGV PPDVPAGAGA PPGLPDAPRN GQEKHFKDEE EDEEEASPDG SGRLNVAGSG GDDGAGGPAV ATAEGNFAT
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Transcription factor that represses the transcription of a wide range of genes involved in cell proliferation and differentiation. Directly and specifically binds to the consensus sequence 5'-[GA][CA]GACCCCCCCCC-3' and represses transcription both by regulating the organization of chromatin and through the direct recruitment of transcription factors to gene regulatory regions. Negatively regulates SMAD4 transcriptional activity in the TGF-beta signaling pathway through these two mechanisms. That is, recruits the chromatin regulator HDAC1 to the SMAD4-DNA complex and in parallel prevents the recruitment of the transcriptional activators CREBBP and EP300. Collaborates with transcription factors like RELA to modify the accessibility of gene transcription regulatory regions to secondary transcription factors. Also directly interacts with transcription factors like SP1 to prevent their binding to DNA. Functions as an androgen receptor/AR transcriptional corepressor by recruiting NCOR1 and NCOR2 to the androgen response elements/ARE on target genes. Thereby, negatively regulates androgen receptor signaling and androgen-induced cell proliferation. Involved in the switch between fetal and adult globin expression during erythroid cells maturation. Through its interaction with the NuRD complex regulates chromatin at the fetal globin genes to repress their transcription. Specifically represses the transcription of the tumor suppressor ARF isoform from the CDKN2A gene. Efficiently abrogates E2F1-dependent CDKN2A transactivation. Regulates chondrogenesis through the transcriptional repression of specific genes via a mechanism that also requires histone deacetylation. Regulates cell proliferation through the transcriptional regulation of genes involved in glycolysis. Involved in adipogenesis through the regulation of genes involved in adipocyte differentiation. Plays a key role in the differentiation of lymphoid progenitors into B and T lineages. Promotes differentiation towards the B lineage by inhibiting the T-cell instructive Notch signaling pathway through the specific transcriptional repression of Notch downstream target genes. Also regulates osteoclast differentiation. May also play a role, independently of its transcriptional activity, in double-strand break repair via classical non-homologous end joining/cNHEJ. Recruited to double-strand break sites on damage DNA, interacts with the DNA-dependent protein kinase complex and directly regulates its stability and activity in DNA repair. May also modulate the splicing activity of KHDRBS1 toward BCL2L1 in a mechanism which is histone deacetylase-dependent and thereby negatively regulates the pro-apoptotic effect of KHDRBS1.
  • 基因功能参考文献：
    1. LRF is expressed mainly in oligodendrocytes but is not required for oligodendrocyte repopulation of demyelinated lesions. Furthermore, LRF can modulate the extent of remyelination, potentially by contributing to interactions regulating transcription PMID: 28556945
    2. Alveolar macrophages deficient in Zbtb7a failed to induce antibody and T cell responses. PMID: 28701473
    3. this study shows that Thpok and LRF are redundantly required to maintain the size and functions of the postthymic Treg pool PMID: 28754678
    4. This study identified Zbtb7a as a novel direct target for miR-125a by gene expression profiling in miR-125a knockout mice combined with bioinformatics target prediction, and elucidated the role of Zbtb7a in oncogenesis. PMID: 26713860
    5. This study demonstrated an unexpected transcription-independent function for LRF in the classical non-homologous end joining pathway of double-strand break repair. PMID: 26446488
    6. LRF short hairpin RNA suppressed the expression of LRF protein in a glioma cell line compared to the negative control vector-transfected glioma cells. LRF knockdown also reduced cell viability and enhanced cisplatin-induced apoptosis in glioma cells. PMID: 26081031
    7. Data indicate that transcription factors Thpok and LRF redundantly maintain CD4+ lineage integrity. PMID: 25129370
    8. Findings demonstrate that Pokemon suppresses Sprouty1 expression through a miR-21-mediated mechanism, affecting the growth and proliferation of liver cancer cells. PMID: 23355454
    9. Data show that Notch1/Dll4-mediated T-cell instructive signals to long-term HSCs (LT-HSCs) are suppressed by Lrf expression in the bone marrow microenvironment. PMID: 23134786
    10. These results support a critical role for LRF in transcriptional control of differentiation in oligodendrocyte lineage cells during developmental myelination in the CNS. PMID: 22615173
    11. T cells lacking Thpok, which only displayed LRF-dependent functions, contributed to multiple effector responses, both in vitro and in vivo, with the notable exception of Th2 cell responses that control extracellular parasites PMID: 23041065
    12. study shows that LRF plays stage-specific distinct roles in osteoclast differentiation, exemplifying the delicate transcriptional regulation at work in lineage commitment PMID: 22308398
    13. Data demonstrate that the transcription factor leukemia/lymphoma-related factor (LRF) forms an obligate dimer in B cells and regulates mature B cell lineage fate and humoral immune responses via distinctive mechanisms. PMID: 21646720
    14. analysis of the miRNA-mediated interaction between leukemia/lymphoma-related factor (LRF) and alternative splicing factor/splicing factor 2 (ASF/SF2) affects cell senescence and apoptosis PMID: 20923760
    15. may contribute to adipogenesis through influencing the switch from cellular proliferation to terminal differentiation PMID: 14701838
    16. LRF is the first transcription factor found to bind directly to the COMP gene promoter, to recruit HDAC1, and to regulate both COMP gene expression and chondrogenic differentiation PMID: 15337766
    17. Pokemon can specifically repress the transcription of the tumour suppressor gene ARF through direct binding PMID: 15662416
    18. findings demonstrate that LRF is key for instructing early lymphoid progenitors to develop into B lineage cells by repressing T cell-instructive signals produced by the cell-fate signal protein, Notch PMID: 17495164
    19. LRF is post-transcriptionally regulated by miR-20a. PMID: 18596985
    20. Proto-oncogene FBI-1 (Pokemon) and SREBP-1 synergistically activate transcription of fatty-acid synthase gene PMID: 18682402

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  • 亚细胞定位：
    Nucleus.
  • 组织特异性：
    Widely expressed. In normal thymus, expressed in medullary epithelial cells and Hassle's corpuscles (at protein level). In the spleen, mainly expressed in the white pulp germinal centers (at protein level). Up-regulated in thymic lymphomas.
  • 数据库链接：

    KEGG: mmu:16969

    STRING: 10090.ENSMUSP00000047333

    UniGene: Mm.20920