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  4. Recombinant Mouse Wilms tumor protein 1-interacting protein (Wtip)

Recombinant Mouse Wilms tumor protein 1-interacting protein (Wtip)

  • 货号：
    CSB-YP752651MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP752651MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP752651MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP752651MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP752651MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Wtip; Wilms tumor protein 1-interacting protein; WT1-interacting protein
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-398
  • 氨基酸序列
    MQRSRTAADD AALLLAGLGL RESEPTAGSP GRVRRGPRAV DEAAPASGRR GKGGCGGPEA APDVPSRPER GPRASLAGSD GGSARSSGIS LGYDQRHGPG PGPPSGGSAR SSVSSLGSRG SAGACADLLP PGVGPAPARS PEPAQFPFPL PSLPLPPGRE GGPSAAERRL EALTRELERA LEARTARDYF GICIKCGLGI YGARQACQAM GSLYHTDCFI CDSCGRRLRG KAFYNVGEKV YCQEDFLYSG FQQTADKCSV CGHLIMEMIL QALGKSYHPG CFRCSVCNEC LDGVPFTVDV DNNIYCVRDY HTVFAPKCAS CARPILPAQG CETTIRVVSM DRDYHVECYH CEDCGLQLSG EEGRRCYPLE GHLLCRRCHL RRLGQGPLPS PAVHVTEL
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Adapter or scaffold protein which participates in the assembly of numerous protein complexes and is involved in several cellular processes such as cell fate determination, cytoskeletal organization, repression of gene transcription, cell-cell adhesion, cell differentiation, proliferation and migration. Positively regulates microRNA (miRNA)-mediated gene silencing. Negatively regulates Hippo signaling pathway and antagonizes phosphorylation of YAP1. Acts as a transcriptional corepressor for SNAI1 and SNAI2/SLUG-dependent repression of E-cadherin transcription. Acts as a hypoxic regulator by bridging an association between the prolyl hydroxylases and VHL enabling efficient degradation of HIF1A. In podocytes, may play a role in the regulation of actin dynamics and/or foot process cytoarchitecture. In the course of podocyte injury, shuttles into the nucleus and acts as a transcription regulator that represses WT1-dependent transcription regulation, thereby translating changes in slit diaphragm structure into altered gene expression and a less differentiated phenotype. Involved in the organization of the basal body. Involved in cilia growth and positioning.
  • 基因功能参考文献：
    1. implicate Asxl1 in the maintenance of podocyte structure via its association with Wtip and in the regulation of WT1 signaling during early kidney development PMID: 26385183
    2. Dendrin is dispensable for the function of the normal glomerular filtration barrier, and it interacts with Wtip and Gadd45a. PMID: 24376653
    3. Wtip regulates the stable formation of cell adhesions to both extracellular matrix and neighboring cells and suggest that Wtip is necessary for normal glomerular filtration barrier function PMID: 21900451
    4. Podocyte injury induces nuclear translocation of WTIP via microtubule-dependent transport PMID: 20086015
    5. WTIP monitors slit diaphragm protein assembly as part of a multiple protein complex, linking this specialized adhesion junction to the actin cytoskeleton, and shuttles into the nucleus after podocyte injury PMID: 14736876
    6. Full-length Ror2 recruits Wtip to the cell membrane, a mutant involved in human disease fails to do so. PMID: 19785987

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  • 亚细胞定位：
    Cell junction, focal adhesion. Cell junction, adherens junction. Nucleus. Cytoplasm, cytosol. Cell membrane.
  • 蛋白家族：
    Zyxin/ajuba family
  • 组织特异性：
    Expressed in kidney, lung, eye and ovary. In kidney, restricted to podocytes.
  • 数据库链接：

    KEGG: mmu:101543

    STRING: 10090.ENSMUSP00000047623

    UniGene: Mm.422738