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  4. Recombinant Mouse WD repeat-containing protein 1 (Wdr1)

Recombinant Mouse WD repeat-containing protein 1 (Wdr1)

  • 货号：
    CSB-YP025995MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP025995MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP025995MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP025995MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP025995MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Wdr1
  • Uniprot No.：
  • 别名：
    Wdr1WD repeat-containing protein 1; Actin-interacting protein 1; AIP1
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-606
  • 氨基酸序列
    PYEIKKVFA SLPQVERGVS KILGGDPKGD HFLYTNGKCV ILRNIDNPAI ADIYTEHAHQ VVVAKYAPSG FYIASGDISG KLRIWDTTQK EHLLKYEYQP FAGKIKDIAW TEDSKRIAVV GEGREKFGAV FLWDTGSSVG EITGHNKVIN SVDIKQTRPY RLATGSDDNC AAFFEGPPFK FKFTIGDHSR FVNCVRFSPD GNRFATASAD GQIFIYDGKT GEKVCALGES KAHDGGIYAI SWSPDSTHLL SASGDKTSKI WDVNVNSVVS TFPMGSNVLD QQLGCLWQKD HLLSISLSGY INYLDKNNPS KPLRVIKGHS KSIQCLTVHR NGGKSYIYSG SHDGHINYWD SETGENDSFS GKGHTNQVSR MTVNESEQLV SCSMDDTVRY TNLTLRDYSG QGVVKLDVQP KCVAVGPGGY TVVVCIGQIV LLKDQKKCFS IDNPGYEPEV VAVHPGGDTV AVGGTDGNVR VYSILASTLK DEGKLLEAKG PVTDVAYSHD GAFLAVCDAS KVVTVFSVAD GYSENNVFYG HHAKIVCLAW SPDNEHFASG GMDMMVYVWT LSDPETKVKI QDAHRLHHVS SLAWLDEHTL VTTSHDASVK EWTITY
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Induces disassembly of actin filaments in conjunction with ADF/cofilin family proteins. Enhances cofilin-mediated actin severing. Involved in cytokinesis. Involved in chemotactic cell migration by restricting lamellipodial membrane protrusions. Involved in myocardium sarcomere organization. Required for cardiomyocyte growth at the postnatal and maintenance at the adult stage. Involved in neutrophil actin dynamics and migration. Involved in megakaryocyte maturation and platelet shedding. Required for the establishment of planar cell polarity (PCP) during follicular epithelium development and for cell shape changes during PCP; the function seems to implicate cooperation with CFL1 and/or DSTN/ADF. Involved in the generation/maintenance of cortical tension. Involved in assembly and maintenance of epithelial apical cell junctions and plays a role in the organization of the perijunctional actomyosin belt.
  • 基因功能参考文献：
    1. Following platelets spreading on fibrinogen substrate, Wdr-1 translocates to the cytoskeleton in association with cofilin-1. In Wdr-1-deficient platelets, cofilin-1 is aberrantly localized throughout the cytoplasm and there is no significant change following adhesion to fibrinogen substrate. PMID: 29995657
    2. essential for talin-induced activation of alphaIIbbeta3 during platelet activation PMID: 27627652
    3. WDR1 interacts with Lim domain kinase 1 (LIMK1), a well known phosphorylation kinase of Cofilin. PMID: 27994054
    4. Wdr1 and cofilin/destrin-mediated actomyosin remodelling are essential for generating or maintaining cortical tension within the developing epidermal sheet and driving the cell shape and planar orientation changes. PMID: 25915128
    5. Wdr1-regulated actin dynamics play essential roles in heart function in mice. F-actin accumulations caused by Wdr1 deletion result in disruption of sarcomeric structure. PMID: 24840128
    6. The QKI RNA-binding proteins regulate oligodendrocyte differentiation by modulating the expression of AIP-1. PMID: 20631256
    7. studies establish an essential requirement for Wdr1 in megakaryocytes and neutrophils PMID: 17515402

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  • 亚细胞定位：
    Cytoplasm, cytoskeleton. Cell projection, podosome.
  • 蛋白家族：
    WD repeat AIP1 family
  • 数据库链接：

    KEGG: mmu:22388

    STRING: 10090.ENSMUSP00000005234

    UniGene: Mm.2654