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  4. Recombinant Mouse Ubl carboxyl-terminal hydrolase 18 (Usp18)

Recombinant Mouse Ubl carboxyl-terminal hydrolase 18 (Usp18)

  • 中文名称：
    小鼠Usp18重组蛋白
  • 货号：
    CSB-YP894351MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Usp18重组蛋白
  • 货号：
    CSB-EP894351MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Usp18重组蛋白
  • 货号：
    CSB-EP894351MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Usp18重组蛋白
  • 货号：
    CSB-BP894351MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Usp18重组蛋白
  • 货号：
    CSB-MP894351MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Usp18
  • Uniprot No.：
  • 别名：
    Usp18; Ubp43Ubl carboxyl-terminal hydrolase 18; EC 3.4.19.-; 43 kDa ISG15-specific protease; ISG15-specific-processing protease; Ubl thioesterase 18
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-368
  • 氨基酸序列
    MGKGFGLLRK PCQSVVAEPQ QYSALEEERT MKRKRVLSRD LCSAWDSPHG LVGLHNIGQT CCLNSLLQVF MMNMDFRMIL KRITVPRSAE ERKRSVPFQL LLLLEKMQDS RQKAVLPTEL VQCLQKYNVP LFVQHDAAQL YLTIWNLTKD QITDTDLTER LQGLFTIWTQ ESLICVGCTA ESSRRSKLLT LSLPLFDKDA KPLKTLEDAL RCFVQPKELA SSDMCCESCG EKTPWKQVLK LTHLPQTLTI HLMRFSARNS RTEKICHSVN FPQSLDFSQV LPTEEDLGDT KEQSEIHYEL FAVIAHVGMA DFGHYCAYIR NPVDGKWFCF NDSHVCWVTW KDVQCTYGNH RYRWRETAYL LVYTKTGS
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Involved in the negative regulation of the inflammatory response triggered by type I interferon. Upon recruitment by STAT2 to the type I IFN receptor subunit IFNAR2 interferes with the assembly of the ternary interferon-IFNAR1-IFNAR2 complex and acts as a negative regulator of the type I IFN signaling pathway. Also regulates protein ISGylation. Can efficiently cleave only ISG15 fusions including native ISG15 conjugates linked via isopeptide bonds. Necessary to maintain a critical cellular balance of ISG15-conjugated proteins in both healthy and stressed organisms.
  • 基因功能参考文献：
    1. Usp18 protects the cells from oxidative stress-induced apoptosis which may be through the regulation of p53 and caspase 3. PMID: 28557172
    2. associates with and deubiquitinates TAK1 to protect against hepatic steatosis, insulin resistance, and the inflammatory response PMID: 28718215
    3. this study identifies the osteopenia phenotype of mice lacking Usp18, which is known as a negative regulator of type I IFN signaling PMID: 27016605
    4. USP18 has a protective role in pathological cardiac remodeling in mouse. PMID: 27572150
    5. USP18's ISG15 specificity is mediated by a small interaction interface. PMID: 28165509
    6. findings show that multiple inflammatory stimuli can modulate interferon stimulated gene expression and thus inhibit hepatocyte interferon signaling via USP18 induction PMID: 27009955
    7. Increased ISGylation was accompanied by enhanced viral resistance without causing detrimental side effects suggesting that USP18 protease inhibition might be a suitable antiviral strategy PMID: 25805508
    8. USP18 null mice develop leiomyosarcoma recapitulating key features of clinical leiomyosarcomas and patients with reduced-USP18 tumor levels have an unfavorable outcome. PMID: 26555296
    9. USP18 lack in microglia causes destructive interferonopathy of the mouse brain. PMID: 25896511
    10. results suggest that increasing ISGylation by specific inhibition of USP18 protease activity could constitute a promising antiviral strategy with only a minimal risk of severe adverse effects PMID: 25605921
    11. USP18 is crucial for IFN-gamma-mediated inhibition of B16 melanoma tumorigenesis and antitumor immunity. PMID: 24884733
    12. Usp18-driven enforced viral replication in dendritic cells can break immunological tolerance and critically influences induction of autoimmunity. PMID: 24204252
    13. Hypersensitivity of Usp18 knockout in mammary epithelial cells to IFN-gamma enhances Cxcl10 expression and protects against mammary tumour progression. PMID: 23681607
    14. Usp18 upregulation was associated with Wilms Tumor. PMID: 23291318
    15. although UBP43 depletion can cause hypersensitivity to interferon-alpha/beta-mediated apoptosis in a broad range of cell types, the downstream pathway may vary depending on the cell type. PMID: 22683641
    16. Usp18 modulates conventional CD11b+ dendritic cell development via its inhibitory effect on type I IFN signaling. PMID: 22491252
    17. These results show the impact of USP18 in the development of Salmonella-induced typhlitis. PMID: 21614019
    18. UBP43 (USP18) specifically removes ISG15 from conjugated proteins PMID: 11788588
    19. UBP43 plays a critical role in maintaining the homeostatic balance of ISG15-conjugated protein, and that regulation of cellular levels of ISG15 protein modification is essential for brain cell function. PMID: 12208842
    20. These findings identify UBP43 as a novel negative regulator of IFN signaling and suggest the involvement of protein ISGylation in the regulation of the JAK-STAT pathway. PMID: 12600939
    21. UBP43 protein levels are regulated by proteolysis via the SCFSkp2 ubiquitin ligase PMID: 15342634
    22. UBP43 (USP18), an ISG15 protease, and possibly protein ISGylation have a role in innate immunity against viral infection. PMID: 15531891
    23. The phenotype of UBP43(-/-) mice was not rescued by the absence of ISG15, as evident from unchanged mortality, neurological symptoms, and occurrence of hydrocephalus. PMID: 16314524
    24. Lack of UBP43, but not the increase of protein ISGylation, is related to the increased IFN signaling in Ubp43-deficient mice. PMID: 16382139
    25. Results show that Ubp43 negatively regulates type I interferon signaling independent of its isopeptidase activity towards ISG15. PMID: 16710296
    26. Deletion of Ubp43 causes an increase in free and conjugated Isg15 at the feto-maternal interface. PMID: 17381847
    27. Steady-state level of hepatitis B virus (HBV)DNA is substantially reduced in UBP43-deficient mice in comparison to wild-type controls and suggests that HBV infection is more rapidly cleared if UBP43 expression is reduced. PMID: 18941237
    28. Epstein-Barr virus independent dysregulation of UBP43 expression alters interferon-stimulated gene expression in Burkitt lymphoma PMID: 19551150
    29. Results identify the inhibitor USP18/UBP43 as the key mediator of alpha-interferon-induced, long-lasting refractoriness of JAK-STAT signaling in the mouse liver. PMID: 19564419

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  • 蛋白家族：
    Peptidase C19 family
  • 数据库链接：

    KEGG: mmu:24110

    STRING: 10090.ENSMUSP00000032198

    UniGene: Mm.326911