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  4. Recombinant Mouse Ubiquitin-like protein ISG15 (Isg15)

Recombinant Mouse Ubiquitin-like protein ISG15 (Isg15)

  • 中文名称：
    小鼠Isg15重组蛋白
  • 货号：
    CSB-YP723739MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Isg15重组蛋白
  • 货号：
    CSB-EP723739MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Isg15重组蛋白
  • 货号：
    CSB-BP723739MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Isg15
  • Uniprot No.：
  • 别名：
    Isg15; G1p2; Ucrp; Ubiquitin-like protein ISG15; Interferon-induced 15 kDa protein; Interferon-induced 17 kDa protein; IP17; Ubiquitin cross-reactive protein
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full Length
  • 表达区域：
    1-155
  • 氨基酸序列
    MAWDLKVKML GGNDFLVSVT NSMTVSELKK QIAQKIGVPA FQQRLAHQTA VLQDGLTLSS LGLGPSSTVM LVVQNCSEPL SILVRNERGH SNIYEVFLTQ TVDTLKKKVS QREQVHEDQF WLSFEGRPME DKELLGEYGL KPQCTVIKHL RLRGG
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Ubiquitin-like protein which plays a key role in the innate immune response to viral infection either via its conjugation to a target protein (ISGylation) or via its action as a free or unconjugated protein. ISGylation involves a cascade of enzymatic reactions involving E1, E2, and E3 enzymes which catalyze the conjugation of ISG15 to a lysine residue in the target protein. Its target proteins include SERPINA3G/SPI2A, JAK1, MAPK3/ERK1, PLCG1, TRIM25, STAT5A, MAPK1/ERK2 and globin. Isgylation of the viral sensor IFIH1/MDA5 promotes IFIH1/MDA5 oligomerization and triggers activation of innate immunity against a range of viruses, including coronaviruses, flaviviruses and picornaviruses. Can also isgylate: DDX58/RIG-I which inhibits its function in antiviral signaling response, IRF3 which inhibits its ubiquitination and degradation as well as EIF4E2 which enhances its cap structure-binding activity and translation-inhibition activity. Exhibits antiviral activity towards both DNA and RNA viruses, including influenza A and B virus, sindbis virus (SV) and herpes simplex type-1 (HHV-1). Plays a significant role in the control of neonatal Chikungunya virus (CHIKV) infection by acting as a putative immunomodulator of proinflammatory cytokines. Protects mice against the consequences of Chikungunya virus infection by downregulating the pathogenic cytokine response, often denoted as the cytokine storm. Plays a role in erythroid differentiation. The secreted form of ISG15 can: induce natural killer cell proliferation, act as a chemotactic factor for neutrophils and act as a IFN-gamma-inducing cytokine playing an essential role in antimycobacterial immunity. The secreted form acts through the integrin ITGAL/ITGB2 receptor to initiate activation of SRC family tyrosine kinases including LYN, HCK and FGR which leads to secretion of IFNG and IL10; the interaction is mediated by ITGAL.
  • 基因功能参考文献：
    1. ISGylation is a ubiquitin-like modification that controls exosome release. ISGylation induction decreases microvesicular body numbers and impairs exosome secretion. Specifically, ISGylation of the MVB protein TSG101 induces its aggregation and degradation, being sufficient to impair exosome secretion. PMID: 27882925
    2. Murine Isg15 does not control Usp18 accumulation and IFN-alpha/beta signaling. PMID: 27193971
    3. Extracellular ISG15 signals cytokine secretion through the LFA-1 integrin receptor (CD11a/CD18) in natural killer cells. PMID: 29100055
    4. results demonstrate that ISG15 governs the dynamic functionality of mitochondria, specifically, OXPHOS and mitophagy, broadening its physiological role as an antiviral agent. PMID: 29077752
    5. Loss of ISG15 expression and type I IFN signaling have transient effects on bacterial burden during M. tuberculosis infection in mice PMID: 28087453
    6. ISG15 acts as an immunomodulator in the cornea and plays a critical role in controlling fungal keratitis. PMID: 28599020
    7. USP18's ISG15 specificity is mediated by a small interaction interface. PMID: 28165509
    8. Posttranslational protein modification by ISG15 plays an important role in antiviral defense PMID: 25805508
    9. These studies provide evidence, ISG15 is elevated following TBI in mice, preceding MLCK elevation, development of BBB disruption, and cerebral edema. PMID: 25669448
    10. Kruppel-like factor 9 (KLF9) is a haploinsufficient tumor suppressor in the ApcMin/+ mouse colon by suppressing expression of ISG15, an apoptosis-inhibiting cytokine. PMID: 26210742
    11. Propose that Isg15-dependent degradation of p53 represents an alternative mechanism of controlling p53 protein levels, and, thus, it is an attractive pathway for drug discovery. PMID: 24844324
    12. Embryo mortality occurs in pregnant Isg15(-/-) mice, is exacerbated by environmental insults like maternal hypoxia, and might result from impaired early decidualization, vascular development, and formation of the labyrinth. PMID: 25505199
    13. Enhanced resistance upon influenza B infection in USP18(C61A/C61A) mice was completely reversed in USP18(C61A/C61A) mice, which additionally lack ISG15, providing evidence that the observed reduction in viral titers is ISG15 dependent PMID: 25605921
    14. s found that ISG15 protects mice from influenza A virus and Sendai virus induced lethality by a conjugation-dependent mechanism in both of these models. PMID: 25320315
    15. study demonstrated that ISG15 had an anti-apoptotic effect on the beta cell line MIN6; conclusion is consistent with previous reports that ISG15 has a protective role PMID: 25031023
    16. The ISG15 conjugation system represents a critical innate response mechanism in cardiomyocytes to fight the battle against invading pathogens, limiting inflammatory cardiomyopathy, heart failure, and death. PMID: 25165091
    17. ISG15 does not appear to play a key role in IFN-beta-mediated C2C12 myoblast cell fusion PMID: 23750257
    18. Interferon-stimulated gene 15 (ISG15) mitigates MNV-1 replication. PMID: 24899198
    19. Protein interferon-stimulated gene 15 conjugation delays but does not overcome coronavirus proliferation in a model of fulminant hepatitis. PMID: 24648452
    20. these results indicate an essential role of ISG15 in the cellular immune antiviral response PMID: 24137104
    21. Isg15 regulates the expression of proteins that directly affect the HCV replication. The pro-viral effect of Isg15 in HCV replication might be mediated through these effector proteins. PMID: 24416772
    22. Activation of double-stranded RNA-activated protein kinase (PKR) by interferon-stimulated gene 15 (ISG15) modification down-regulates protein translation PMID: 23229543
    23. vaccination against ISG15 results in significant CD8-mediated reductions in both primary and metastatic mammary tumor burden. PMID: 22057675
    24. Data show that the HerC6 N-terminal RCC1-like domain (RLD) allows ISG15 conjugation. PMID: 22272257
    25. mHERC6 is essential for endogenous murine ISGylation and thus represents the dominant ISG15 E3 ligase in mice. PMID: 22138649
    26. These findings suggest that ISG15 plays an anti-dengue virus and West Nile virus function via protein ISGylation. PMID: 21992229
    27. ISG15 is induced during late erythroid differentiation. PMID: 22022510
    28. increased protein ISGylation might be an endogenous neuroprotective adaptation to minimize poststroke brain damage PMID: 21847135
    29. expression of the ISG15 is elevated in A-T cells derived from various A-T patients, as well as in brain tissues derived from the ATM knockout mice and A-T patients, suggesting that ATM negatively regulates the ISG15 pathway PMID: 21298066
    30. In addition to cellular ISGylation targets, recent reports implicate viral proteins as targets of ISG15 modification; these studies provide fresh insights into the antiviral mechanisms of ISG15.[Review] PMID: 20946978
    31. replication of hepatitis C virus was inhibited by overexpression of ISG15 PMID: 20810994
    32. Data show that contrary to earlier reports, up to 50% of the fetuses die between 7.5 and 12.5 d post coitum (dpc) in Isg15(-/-) mothers when mated to Isg15(-/-) fathers. PMID: 20660068
    33. Ectopic expression of the Herc5-mediated ISGylation system could distinctly potentiate IFN-beta-induced antiviral effects against influenza A virus. PMID: 20385878
    34. The s demonstrate that influenza B virus NS1 protein potently antagonizes human but not mouse ISGylation, a property dependent on B/NS1 binding the N-terminal domain of human but not mouse ISG15. PMID: 20219937
    35. UBP43 (USP18) specifically removes ISG15 from conjugated proteins PMID: 11788588
    36. lipopolysaccharide activation of expression via interferon regulatory factor 3 PMID: 11854279
    37. UBP43 plays a critical role in maintaining the homeostatic balance of ISG15-conjugated protein, and that regulation of cellular levels of ISG15 protein modification is essential for brain cell function. PMID: 12208842
    38. Isg15 is one of few gene products identified in murine implantation sites to require presence of the conceptus and not simply differentiation of the stroma. PMID: 12810567
    39. results suggest that the ubiquitin family protein ISG15/UCRP has novel functions in neutrophil-mediated immune mechanisms PMID: 12963022
    40. Ubc8 is a major ISG15-conjugating enzyme responsible for protein ISGylation upon interferon stimulation PMID: 15485925
    41. ISG15 is dispensable for STAT1 and interferon signaling PMID: 16024773
    42. These data indicate that ISGylation targets proteins found in several fundamentally important cellular pathways and will contribute to understanding the physiologic role of interferon-induced ISG15 and ISG15 conjugation. PMID: 16139798
    43. ISG15 expression protected against Sindbis virus-induced lethality and decreased Sindbis virus replication in multiple organs without inhibiting the spread of virus throughout the host. PMID: 16254333
    44. The phenotype of UBP43(-/-) mice was not rescued by the absence of ISG15, as evident from unchanged mortality, neurological symptoms, and occurrence of hydrocephalus. PMID: 16314524
    45. Results show that Ubp43 negatively regulates type I interferon signaling independent of its isopeptidase activity towards ISG15. PMID: 16710296
    46. Virus-mediated subversion of the antiviral response by proteolysis of IRF-3 is counteracted by induction of ISG15 expression. PMID: 16914094
    47. ISG15 is a novel antiviral molecule with activity against both RNA and DNA viruses provides a target for the development of therapies against important human pathogens PMID: 17227866
    48. Isg15 and its conjugates are present in implantation sites during mid to late gestation in mice. PMID: 17381847
    49. Leishmania braziliensis infection triggered transcription and phosphorylation of STAT molecules and IFN-stimulated gene 15 PMID: 18490754
    50. nitrosylation of ISG15 enhances target protein ISGylation PMID: 18606809

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  • 亚细胞定位：
    Cytoplasm. Secreted.
  • 数据库链接：

    KEGG: mmu:100038882

    STRING: 10090.ENSMUSP00000082548

    UniGene: Mm.451527