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  4. Recombinant Mouse Ubiquitin carboxyl-terminal hydrolase isozyme L3 (Uchl3)

Recombinant Mouse Ubiquitin carboxyl-terminal hydrolase isozyme L3 (Uchl3)

  • 中文名称：
    小鼠Uchl3重组蛋白
  • 货号：
    CSB-YP884940MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Uchl3重组蛋白
  • 货号：
    CSB-EP884940MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Uchl3重组蛋白
  • 货号：
    CSB-EP884940MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Uchl3重组蛋白
  • 货号：
    CSB-BP884940MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Uchl3重组蛋白
  • 货号：
    CSB-MP884940MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Uchl3
  • Uniprot No.：
  • 别名：
    Uchl3Ubiquitin carboxyl-terminal hydrolase isozyme L3; UCH-L3; EC 3.4.19.12; Ubiquitin thioesterase L3
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-230
  • 氨基酸序列
    MEGQRWLPLE ANPEVTNQFL KQLGLHPNWQ FVDVYGMEPE LLSMVPRPVC AVLLLFPITE KYEVFRTEEE EKIKSQGQDV TSSVYFMKQT ISNACGTIGL IHAIANNKDK MHFESGSTLK KFLEESVSMS PEERAKFLEN YDAIRVTHET SAHEGQTEAP SIDEKVDLHF IALVHVDGHL YELDGRKPFP INHGKTSDET LLEDAIEVCK KFMERDPDEL RFNAIALSAA
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Deubiquitinating enzyme (DUB) that controls levels of cellular ubiquitin through processing of ubiquitin precursors and ubiquitinated proteins. Thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of either ubiquitin or NEDD8. Has a 10-fold preference for Arg and Lys at position P3'', and exhibits a preference towards 'Lys-48'-linked ubiquitin chains. Deubiquitinates ENAC in apical compartments, thereby regulating apical membrane recycling. Indirectly increases the phosphorylation of IGFIR, AKT and FOXO1 and promotes insulin-signaling and insulin-induced adipogenesis. Required for stress-response retinal, skeletal muscle and germ cell maintenance. May be involved in working memory. Can hydrolyze UBB(+1), a mutated form of ubiquitin which is not effectively degraded by the proteasome.
  • 基因功能参考文献：
    1. impaired UCH-L3 function may contribute to the accumulation of full length UBB(+1) in various pathologie PMID: 21762696
    2. Results suggest that Uch-L3 enhances osteoblast differentiation through the stabilization of Smad1 signaling PMID: 21453705
    3. Polyubiquitinated proteins accumulate in skeletal muscle as well as in mouse embryonic fibroblasts derived from Uchl3-deficient mice. PMID: 20380862
    4. Results suggest that UCH-L3 promotes adipogenesis by enhancing insulin signaling in a hydrolase activity-dependent manner. PMID: 19837878
    5. The role of ubiquitin C-terminal hydrolase (UCH)-L3 in the regulation of AMP-activated protein kinase activity and whole-body energy metabolism are reportedl PMID: 19671667
    6. UCH-L3 regulates the apical membrane recycling of the epithelial sodium channel PMID: 17967898
    7. Data show that Uchl3 and mitofilin are differentially expressed in the hippocampi of inbred senescence-acclerated mice compared to normally-aging mice. PMID: 18307031

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  • 亚细胞定位：
    Cytoplasm.
  • 蛋白家族：
    Peptidase C12 family
  • 组织特异性：
    Ubiquitously expressed, with highest levels in brain, liver, heart, thymus, kidney and testis. Highly expressed in the cauda epididymidis, in meiotic pachytene spermatocytes and post-meiotic spematids. In the retina, enriched in the photoreceptor inner se
  • 数据库链接：

    KEGG: mmu:50933

    STRING: 10090.ENSMUSP00000002289

    UniGene: Mm.275970