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  4. Recombinant Mouse Ubiquitin carboxyl-terminal hydrolase isozyme L1 (Uchl1)

Recombinant Mouse Ubiquitin carboxyl-terminal hydrolase isozyme L1 (Uchl1)

  • 中文名称：
    小鼠Uchl1重组蛋白
  • 货号：
    CSB-YP889925MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Uchl1重组蛋白
  • 货号：
    CSB-EP889925MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Uchl1重组蛋白
  • 货号：
    CSB-EP889925MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Uchl1重组蛋白
  • 货号：
    CSB-BP889925MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Uchl1重组蛋白
  • 货号：
    CSB-MP889925MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Uchl1
  • Uniprot No.：
  • 别名：
    Uchl1; Ubiquitin carboxyl-terminal hydrolase isozyme L1; UCH-L1; EC 3.4.19.12; Neuron cytoplasmic protein 9.5; PGP 9.5; PGP9.5; Ubiquitin thioesterase L1
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-220
  • 氨基酸序列
    MQLKPMEINP EMLNKVLAKL GVAGQWRFAD VLGLEEETLG SVPSPACALL LLFPLTAQHE NFRKKQIEEL KGQEVSPKVY FMKQTIGNSC GTIGLIHAVA NNQDKLEFED GSVLKQFLSE TEKLSPEDRA KCFEKNEAIQ AAHDSVAQEG QCRVDDKVNF HFILFNNVDG HLYELDGRMP FPVNHGASSE DSLLQDAAKV CREFTEREQG EVRFSAVALC
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins (Probable). This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity.
  • 基因功能参考文献：
    1. UCH-L1 is required for regulated protein degradation in the kidney by controlling proteasome abundance. Altered proteasome abundance renders renal cells, particularly podocytes and endothelial cells, susceptible to injury. PMID: 28754552
    2. High UCHL1 expression is associated with posterior longitudinal ligament ossification. PMID: 29782494
    3. PKCgamma-mediated down-regulation of UCHL1 alleviates ischemic neuronal injuries by decreasing autophagy via ERK-mTOR pathway. PMID: 28726275
    4. Post-transcriptional loss of UCHL1 following status epilepticus is deleterious to neuronal survival. PMID: 28397067
    5. The UCHL1 may play a role in myogenesis by promoting myoblast proliferation and inhibiting differentiation. PMID: 28803986
    6. our study provides novel insights into the mechanisms of UCH-L1-mediated neurobiological functions and suggests that ubiquitination is an important regulatory signal for TrkB PMID: 28500221
    7. Effects of Ubiquitin C-Terminal Hydrolase L1 (UCH-L1) inhibition on sperm incorporation and cortical tension in mouse eggs. PMID: 26781791
    8. The neuronal marker UCH-L1 is induced in, and specifically augments the oncogene-induced transformation of, germinal center B cells. PMID: 26702068
    9. Pharmacological inhibition of UCHL1 exacerbates rather than ameliorates disease symptoms in a mouse model of SMA. Thus, pharmacological inhibition of UCHL1 is not a viable therapeutic target for SMA. PMID: 25041530
    10. Parkin mediates K63-linked polyubiquitination of UCH-L1 in cooperation with the Ubc13/Uev1a E2 ubiquitin-conjugating enzyme complex and promotes UCH-L1 degradation by the autophagy-lysosome pathway. PMID: 25403879
    11. defective UCHL1 function may be an early contributor to vulnerability of pancreatic beta-cells for protein misfolding and proteotoxicity, hallmark defects in islets of T2DM PMID: 24879150
    12. UCH-L1 facilitates PDGF-BB-induced suppression of autophagic degradation of p21WAF1/Cip1 proteins in cardiac fibroblasts PMID: 24732420
    13. It plays a role in the hypothalamic-pituitary-gonadal axis and in the reproduction. PMID: 24770650
    14. UCHL1 upregulation in ACTN4-associated FSGS. PMID: 24662305
    15. downregulated miR-181b induces neuroprotection against ischemic injury through negatively regulating HSPA5 and UCHL1 protein levels PMID: 23900885
    16. The proteases HtrA2/Omi and UCH-L1 regulate TNF-induced necroptosis. PMID: 24090154
    17. Activation of the NF-kappaB signaling pathway could be the major pathogenic mechanism to up-regulate UCH-L1 in podocyte injury. PMID: 23567262
    18. in AD, Abeta may down-regulate UCH-L1 in the AD brain, which in turn impairs BDNF/TrkB-mediated retrograde signaling, compromising synaptic plasticity and neuronal survival. PMID: 23599427
    19. UCH-L1 physically interacts with CDK1, CDK4, and CDK5, enhancing their kinase activity. PMID: 23543736
    20. UCH-L1 impairs mTORC1 activity toward S6 kinase and 4EBP1. PMID: 23297343
    21. Antisense Uchl1 increases UCHL1 protein synthesis at a post-transcriptional level, hereby identifying a new functional class of lncRNAs PMID: 23064229
    22. Recovery of hypothalamic tuberoinfundibular dopamine neurons from acute toxicant exposure is dependent upon protein synthesis and associated with an increase in parkin and ubiquitin carboxy-terminal hydrolase-L1 expression. PMID: 22342763
    23. New insights into the role of UCHL1 in the mechanism of polyspermy block in mouse ova. PMID: 22223688
    24. Data suggest that UCHL1 has functional roles (i.e., involved in insulin secretion, in ubiquitination, and as brake on cell proliferation) and anti-apoptotic role in pancreatic beta cells under endoplasmic reticulum stress caused by palmitic acid. PMID: 22038515
    25. However, gad mice showed an increased activity during subjective day and a decreased number of orexin A-immunoreactive neurons in the LH compared with the wild type (WT). PMID: 20363052
    26. Data suggest that the activity of oocyte UCHL-1 and -3 contributes to oocyte maturation by regulating the oocyte cortex and meiotic spindle. PMID: 21751213
    27. subfertile Uchl1(gad-/-) mutant mice showed an intriguing pattern of switched UCH localization, with UCHL3 replacing UCHL1 in the oocyte cortex PMID: 21678411
    28. Ubiquitin carboxyterminal hydrolase isozyme L1 showed higher levels in animals with good contextual fear conditioning extinction performance. PMID: 21770032
    29. PTOV1 is associated with UCH-L1 and in response to estrogen stimuli during the mouse oocyte development PMID: 21678139
    30. analysis of a possible mechanistic link between UCH-L1 modification by cyclopentenone prostaglandins and the etiology of neurodegeneration PMID: 20231490
    31. found that, in the absence of UCH-L1, synaptic transmission at the neuromuscular junctions (NMJs) is markedly impaired. PMID: 20080621
    32. PGP9.5-positive neural elements began to appear in the periodontal ligament of the hetrerozygote and wild type mice at day 7 following transection of the inferior alveolar nerve. PMID: 12846558
    33. These data suggest that UCH L1, with avidity and affinity for ubiquitin, insures ubiquitin stability within neurons. PMID: 12913066
    34. UCH-L1 may act during mitotic proliferation of spermatogonial stem cells PMID: 15084487
    35. Results describe the gene expression pattern of the brain of 3-month-old Uch-l1-deficient gracile axonal dystrophy (gad) mice by microarray analysis. PMID: 15207921
    36. UCHL1, and UCHL1-dependent apoptosis, are required for normal spermatogenesis and sperm quality control. PMID: 15744022
    37. UchL1 is expressed in the suprachiasmatic nucleus PMID: 15889617
    38. Results show that a component of the neuronal ubiquitin/proteasomal pathway, ubiquitin C-terminal hydrolase L1 (Uch-L1), is required for normal synaptic and cognitive function. PMID: 16923396
    39. These results may identify UCH-L1 as a candidate for a sperm-oocyte interactive binding or fusion protein on the plasma membrane that functions during the block to polyspermy in mouse oocytes. PMID: 17071595
    40. UCH-L1 is widely expressed in neurons in all brain regions analyzed, including dopamine neurons of the midbrain. In the periphery, UCH-L1 expression remains restricted to neurons in all organs examined. PMID: 17599367
    41. These results provide evidences of UCH-L1/ubiquitination signaling to the testis IR injury in vivo. PMID: 18070598
    42. We propose that UCH-L1-mediated maintenance of the temporal integrity and persistence of CREB phosphorylation underlies these impairments. PMID: 18279321
    43. S18Y variant of UCH-L1 confers a novel antioxidant function in certain Parkinson's disease populations. PMID: 18411255
    44. UCH-L1 regulates bone mineralization during osteogenesis. PMID: 18628613
    45. Results suggest that Uch-L1 and Usp14 may differentially affect the ubiquitin proteasome system and synaptic activity by regulating different pools of ubiquitin in the cell. PMID: 18771733
    46. Studies suggest sln and uchl1 genes as novel targets of AT deficiency and may offer molecular correlates of well documented descriptions of neuromuscular dysfunctions in AT-deficient rodents. PMID: 19204867

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  • 亚细胞定位：
    Cytoplasm. Endoplasmic reticulum membrane; Lipid-anchor.
  • 蛋白家族：
    Peptidase C12 family
  • 组织特异性：
    Expressed in brain, where it is found in neurons but not in oligodendrocytes or astrocytes. Found in the ganglion cell layer and the inner nuclear layer of the retina (at protein level). Expressed in brain and testis. In the brain, expression is at its lo
  • 数据库链接：

    KEGG: mmu:22223

    STRING: 10090.ENSMUSP00000031131

    UniGene: Mm.29807