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  4. Recombinant Mouse Two pore calcium channel protein 2 (Tpcn2), partial

Recombinant Mouse Two pore calcium channel protein 2 (Tpcn2), partial

  • 中文名称：
    小鼠Tpcn2重组蛋白
  • 货号：
    CSB-YP805974MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Tpcn2重组蛋白
  • 货号：
    CSB-EP805974MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Tpcn2重组蛋白
  • 货号：
    CSB-EP805974MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Tpcn2重组蛋白
  • 货号：
    CSB-BP805974MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Tpcn2重组蛋白
  • 货号：
    CSB-MP805974MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Tpcn2
  • Uniprot No.：
  • 别名：
    Tpcn2; Tpc2; Two pore calcium channel protein 2; Voltage-dependent calcium channel protein TPC2
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Intracellular channel initially characterized as a non-selective Ca(2+)-permeable channel activated by NAADP (nicotinic acid adenine dinucleotide phosphate), it is also a highly-selective Na(+) channel activated directly by PI(3,5)P2 (phosphatidylinositol 3,5-bisphosphate). Localizes to the lysosomal and late endosome membranes where it regulates organellar membrane excitability, membrane trafficking, and pH homeostasis. Is associated with a plethora of physiological processes, including mTOR-dependent nutrient sensing, skin pigmentation and autophagy. Ion selectivity is not fixed but rather agonist-dependent and under defined ionic conditions, can be readily activated by both NAADP and PI(3,5)P2. As calcium channel, it increases the pH in the lysosomal lumen, as sodium channel, it promotes lysosomal exocytosis. Plays a crucial role in endolysosomal trafficking in the endolysosomal degradation pathway and is potentially involved in the homeostatic control of many macromolecules and cell metabolites. Unlike the voltage-dependent TPCN1, TPCN2 is voltage independent and can be activated solely by PI(3,5)P2 binding. In contrast, PI(4,5)P2, PI(3,4)P2, PI(3)P and PI(5)P have no obvious effect on channel activation.; (Microbial infection) During Ebola virus (EBOV) infection, controls the movement of endosomes containing virus particles and is required by EBOV to escape from the endosomal network into the cell cytoplasm.
  • 基因功能参考文献：
    1. Under normal Mg(2+) conditions, TPC2 promotes osteoclastogenesis. Under low-Mg(2+) conditions, TPC2 inhibited, rather than promoted, the osteoclast differentiation, and the phosphatidylinositol 3,5-bisphosphate (PI(3,5)P2) signaling pathway played a role in the TPC2 activation under low-Mg(2+) conditions. PMID: 29084844
    2. Data (including data from studies using tissue from knockout mice) suggest that beta-adrenergic stimulation of pancreas leads to glucagon secretion by hierarchy of calcium signaling in glucagon-secreting cell; such signaling is initiated by cAMP-induced Tpc2-dependent calcium release from acidic stores and is further amplified by calcium release from endoplasmic reticulum. PMID: 29563152
    3. Study identifies two-pore channel 2 (TPC2) as the first reported melanosomal cation conductance by directly patch-clamping skin and eye melanosomes. TPC2 is shown to function as a negative regulator of pigmentation by increasing melanosome membrane potential and melanosome acidity. PMID: 27231233
    4. High TPC2 expression is associated with lung metastasis in breast cancer. PMID: 28108508
    5. Electron micrographs of hearts from TPC1/TPC2 double knockout mice showed that cardiomyocytes contained large numbers of immature lysosomes compared to wild-type mice. PMID: 26757341
    6. observations define a role for NAADP and TPC2 at lysosomal/sarcoplasmic reticulum junctions as unexpected but major contributors in the acute actions of beta-adrenergic signaling in the heart PMID: 26438825
    7. TPC2 plays a crucial role in trafficking in the endolysosomal degradation pathway and in fatty liver disease. PMID: 25144390
    8. Genetic ablation of endolysosomal TPC1 or TPC2 channels attenuates glucose- and sulfonylurea-induced membrane currents, depolarization, cytoplasmic Ca2+ signals, and insulin secretion in pancreatic beta cells. PMID: 26152717
    9. Tpcn2 knockout mice demonstrated a significant decrease in fasting glucose levels and insulin response to a glucose challenge. PMID: 25236446
    10. Mice lacking TPC2 display muscle atrophy and altered autophagy. PMID: 25480788
    11. It was concluded that Tpcn1/2(-/-) mice show mature-onset obesity due to reduced lipid availability and use, and a defect in beta-adrenergic receptor signaling, leading to impaired thermogenic activity, in brown adipose tissue. PMID: 25545384
    12. These results demonstrate that a VEGFR2/NAADP/TPC2/Ca(2+) signaling pathway is critical for VEGF-induced angiogenesis PMID: 25331892
    13. Findings indicate that TPC2 have important but distinct roles from TPC1 in the endo-lysosomal pathway. PMID: 25135478
    14. TPC2 signaling inhibits embryonic stem cell entry to early neural progenitors, but is required for late neuronal differentiation. PMID: 23776607
    15. We find alterations in two-pore calcium channel protein expression, with loss of presenilin preventing the formation of a high molecular weight species of TPC1 and TPC2. PMID: 23103503
    16. Study identifies an endolysosomal ATP-sensitive Na channel - a complex formed by two-pore channels TPC1 and TPC2; channel complex detects nutrient status, becomes constitutively open upon nutrient removal and mTOR translocation off the lysosomal membrane, and controls the lysosome's membrane potential, pH stability, and amino acid homeostasis. PMID: 23394946
    17. TPC2 is a novel critical molecule for osteoclastogenesis. PMID: 22833668
    18. Study finds by direct patch-clamp analysis of endolysosomal membranes that PI(3,5)P2 specifically activates TPCs and, unexpectedly, that nicotinic acid adenine dinucleotide phosphate does not. TPC1 and TPC2-mediated currents are selective for Na+, which is the predominant cation in the lysosome. PMID: 23063126
    19. proteins mediate a key NAADP-regulated tissue response brought about by agonist activation of a cell surface recepto PMID: 20547763
    20. analysis of two-pore channel 2 (TPCN2)-mediated Ca2+ currents in isolated lysosomes PMID: 20495006
    21. TPCN2 is a major component of the long-sought lysosomal NAADP-dependent Ca(2+)-release channel. PMID: 19557428

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  • 亚细胞定位：
    Late endosome membrane; Multi-pass membrane protein. Lysosome membrane; Multi-pass membrane protein. Note=Only the acidic lysosomal fraction is sensitive to NAADP.
  • 蛋白家族：
    Calcium channel alpha-1 subunit (TC 1.A.1.11) family, Two pore calcium channel subfamily
  • 组织特异性：
    Widely expressed. Highly expressed in macrophages.
  • 数据库链接：

    KEGG: mmu:233979

    STRING: 10090.ENSMUSP00000061308

    UniGene: Mm.102235