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  4. Recombinant Mouse Transcriptional activator protein Pur-beta (Purb)

Recombinant Mouse Transcriptional activator protein Pur-beta (Purb)

  • 货号：
    CSB-YP019084MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP019084MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP019084MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP019084MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP019084MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Purb
  • Uniprot No.：
  • 别名：
    Purb; Transcriptional activator protein Pur-beta; Purine-rich element-binding protein B; Vascular actin single-stranded DNA-binding factor 2 p44 component
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-324
  • 氨基酸序列
    ADGDSGSER GGGGGGGGGP GGFQPAPRGG GGGGGGPGGE QETQELASKR LDIQNKRFYL DVKQNAKGRF LKIAEVGAGG SKSRLTLSMA VAAEFRDSLG DFIEHYAQLG PSSPEQLAAG AEEGGGPRRA LKSEFLVREN RKYYLDLKEN QRGRFLRIRQ TVNRGGGGFG GGPGPGGLQS GQTIALPAQG LIEFRDALAK LIDDYGGDED ELAGGPGGGA GGPGGGLYGE LPEGTSITVD SKRFFFDVGC NKYGVFLRVS EVKPSYRNAI TVPFKAWGKF GGAFCRYADE MKEIQERQRD KLYERRGGGS GGGDESEGEE VDED
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Has capacity to bind repeated elements in single-stranded DNA such as the purine-rich single strand of the PUR element located upstream of the MYC gene. Participates in transcriptional and translational regulation of alpha-MHC expression in cardiac myocytes by binding to the purine-rich negative regulatory (PNR) element. Modulates constitutive liver galectin-3 gene transcription by binding to its promoter. May play a role in the dendritic transport of a subset of mRNAs. Plays a role in the control of vascular smooth muscle (VSM) alpha-actin gene transcription as repressor in myoblasts and fibroblasts.
  • 基因功能参考文献：
    1. site-directed mutagenesis revealed several basic amino acid residues in the intermolecular (R267) and intramolecular (K82 and R159) subdomains that are essential for Purbeta transcriptional repressor function in Acta2 promoter-reporter assays. In keeping with their diminished Acta2 repressor activity in fibroblasts, purified Purbeta variants containing an R267A mutation exhibited reduced binding affinity for purine-ric... PMID: 27064749
    2. This structural arrangement suggests that the cooperative assembly of the dimeric Purbeta repressor on the sense strand of the ACTA2 enhancer is dictated by the association of each subdomain with distinct purine-rich binding sites within the enhancer. PMID: 23724822
    3. These findings suggest that the highly conserved modular repeats of Purbeta fold to form a core functional domain, which mediates the specific and high affinity binding of the protein to single-stranded DNA. PMID: 20728429
    4. Purbeta is a single-stranded DNA-binding repressor of vascular smooth muscle alpha-actin gene transcription PMID: 12874279
    5. analysis of the quaternary structure of recombinant mouse Purbeta PMID: 17121857
    6. structural role for certain core arginine residues in forming a conformationally stable Pur beta protein capable of physical interactions necessary for smooth muscle alpha-actin gene repression. PMID: 17906292

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  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    PUR DNA-binding protein family
  • 数据库链接：

    KEGG: mmu:19291

    STRING: 10090.ENSMUSP00000136957

    UniGene: Mm.296150