Your Good Partner in Biology Research

  1. 首页
  2. 蛋白
  3. 重组蛋白
  4. Recombinant Mouse Transcriptional activator GLI3 (Gli3)

Recombinant Mouse Transcriptional activator GLI3 (Gli3)

  • 货号：
    CSB-YP730746MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP730746MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP730746MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP730746MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP730746MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Gli3; Transcriptional activator GLI3; GLI3 form of 190 kDa; GLI3-190; GLI3 full-length protein; GLI3FL) [Cleaved into: Transcriptional repressor GLI3R; GLI3 C-terminally truncated form; GLI3 form of 83 kDa; GLI3-83)]
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Has a dual function as a transcriptional activator and a repressor of the sonic hedgehog (Shh) pathway, and plays a role in limb development. The full-length GLI3 form (GLI3FL) after phosphorylation and nuclear translocation, acts as an activator (GLI3A) while GLI3R, its C-terminally truncated form, acts as a repressor. A proper balance between the GLI3 activator and the repressor GLI3R, rather than the repressor gradient itself or the activator/repressor ratio gradient, specifies limb digit number and identity. In concert with TRPS1, plays a role in regulating the size of the zone of distal chondrocytes, in restricting the zone of PTHLH expression in distal cells and in activating chondrocyte proliferation. Binds to the minimal GLI-consensus sequence 5'-GGGTGGTC-3'.
  • 基因功能参考文献：
    1. 5'Hoxd genes and Gli3 are part of an interdigital signalling centre that sets net Bmp signalling levels from different interdigits to coordinately regulate phalanx and joint formation. PMID: 27713395
    2. Gli3 is a suppressor of stem cell proliferation that affects the number and function of mature taste cells, especially Tas1r3+ cells, in adult posterior tongue. PMID: 29415007
    3. Gli3 activity in mouse thymic epithelial cells (TECs) promotes positive selection and differentiation from CD4(+) CD8(+) to CD4(+) CD8(-) single-positive (SP4) cells in the fetal thymus and Gli3 represses Shh constitutive deletion of Gli3, and conditional deletion of Gli3 from TECs, reduced differentiation to SP4, whereas conditional deletion of Gli3 from thymocytes did not. PMID: 29361554
    4. loss of Spop, but not Spopl, disrupts chondrocyte hypertrophy and osteoblast differentiation in the mouse, suggesting the requirement for Spop-mediated protein degradation in mouse skeletal development; overexpressed Spop targets both Gli3FL and Gli3R for ubiquitination and degradation and Spop is an important positive regulator of Ihh signaling and skeletal development PMID: 27930311
    5. mutual interactions between Gli3, Wnt8b, and Fgf17 are crucial elements of the balance between these factors thereby conferring robustness to the patterning process PMID: 26656997
    6. Results demonstrate a negative role of Spop in the level and activity of Gli3, Shh signaling and ventral spinal cord patterning. PMID: 28412462
    7. These results suggest that Tctn1, Tctn2, and Tctn3 are functionally divergent with respect to their role in ciliogenesis and Hedgehog signaling but conserved in neural tube patterning and Gli3 processing. PMID: 28800946
    8. Results indicate that the transcription factor Gli3 (Gli3)-mutant fetal liver (FL) had increased sonic hedgehog (Shh) signaling resulting in decreased B cell development. PMID: 28533268
    9. Our findings show how Nestin drives hedgehog pathway-driven cancers and the tumor-promoting effects of Nestin were mediated by binding to Gli3, a zinc finger transcription factor that negatively regulates hedgehog signaling. PMID: 27496710
    10. Gli3 gene is a direct target for repression by Tgifs during the neural tube patterning. PMID: 27924807
    11. cAMP dependent protein kinase A (PKA) is a key intracellular factor mediating SHH signaling through regulation of GLI3 processing. PMID: 27720745
    12. T-box3 interacts with Kif7 and is required for normal stoichiometry and function of a Kif7/Sufu complex that regulates Gli3 stability and processing. PMID: 27046536
    13. In Six2-positive nephrogenic progenitors, GLI3 repressor decreased progenitor cell proliferation reducing the number of nephrogenic precursor structures in the Pallister-Hall syndrome mouse model. PMID: 26604140
    14. miR-378a-3p suppresses activation of hepatic stellate cells by targeting Gli3 and its expression. PMID: 27001906
    15. Gli3 affects LOT positioning and target area innervation through controlling the development of the piriform cortex. PMID: 26509897
    16. These data demonstrate that GLI3 processing is a major outcome of primary cilia function in dorsal telencephalon morphogenesis. PMID: 26071364
    17. Disruption of the SUFU-GLI-SOX10 regulatory loop disrupts migration of neural crest cells and development of the enteric nervous system in mice. PMID: 26261006
    18. Results showed that the Thr396 residue of Sufu is specifically required for regulation of Gli3 but not Gli2 implying a novel Sufu-mediated mechanism in which Gli2 activator and Gli3 repressor are differentially regulated. PMID: 25760946
    19. Findings emphasize the importance of Gli3 in regulating the transition from neocortex to piriform cortex which has major implications for corticofugal axon pathfinding as this affects the size of the entry zone of corticofugal axons into the striatum PMID: 24014668
    20. This study identified a critical role of the Sall4-Gli3 system at the early steps of limb development for proper development of the appendicular skeletal elements. PMID: 25848055
    21. A role for Gli3R as a mediator of niche establishment and provide insights into the conditions required for proper subventricular zone neurogenic niche formation. PMID: 25127137
    22. we show that the Gli3 hypomorphic mouse mutant Polydactyly Nagoya (Pdn) displays agenesis of the CC and mislocation of the glial and neuronal guidepost cells PMID: 23042737
    23. Hox genes control, in a dose-dependent manner, the type of ossification (long versus short bone) of the limb skeletal elements and this function is mediated at least in part through the modulation of the Gli3 repressor function. PMID: 24930703
    24. Gli3 specifies a cortical environment permissive to the growth of cortical axons at the progenitor level by controlling the formation of subplate neurons. PMID: 22903314
    25. Gli3 regulation of myogenesis is necessary for ischemia-induced angiogenesis. PMID: 24044950
    26. Kif3a controls nephron number via distinct cell lineage-specific mechanisms. PMID: 23762375
    27. VLK plays a role in the Indian hedgehog /GLI3 interactions and that Vlk and Gli3 cooperate to regulate long bone development PMID: 23792766
    28. Sonic Hedgehog patterns the anterioposterior limb primarily through the inhibition of Gli3 repressor formation. PMID: 23644062
    29. Primary cilia and the regulation of Gli activity are necessary for the normal expansion of the cerebral cortex during neural development. PMID: 21976438
    30. Gli3 recruitment to cilia tips was reduced and Sufu/Gli3 dissociation was impaired PMID: 23026747
    31. Collectively, these data strongly suggest that we have identified a set of direct Wnt target genes in the developing hippocampus and provide inside into the genetic hierarchy underlying Wnt-regulated hippocampal development. PMID: 22235033
    32. study demonstrates that Zic3 and Gli3 expression overlap in developing limbs and that Zic3 converts Gli3 from repressor to activator in vitro; results indicate two Gli superfamily members that cause disparate human congenital malformation syndromes interact genetically PMID: 22234993
    33. AKT1-GLI3-VMP1 pathway mediates KRAS oncogene-induced autophagy in cancer cells. PMID: 22535956
    34. Gli3 is required at the neural plate stage to regulate Wnt expression and Wnt/beta-catenin signaling in the presumptive forebrain and confirm its previously proposed role in patterning the anterior neural plate. PMID: 21452227
    35. differential involvement of Gli3 explains the variation in phenotype among Gli3(Xt-J/Xt-J) mammary rudiments, and may help to understand the variation in numbers and positions of mammary glands among mammals PMID: 22046263
    36. Data show that in Shh;Gli3 mutants, adjacent rings of Fgf8 and Wnt3a expression are induced in the diencephalon at the ZLI, reminiscent of the Fgf8/Wnt1-expressing isthmic organizer. PMID: 21925158
    37. The extra-toes spotting mouse embryos have ectopic Shh and Ptch1 expression in the anterior limb. In addition, anterior limb buds show aberrant Gli3 processing, consistent with perturbed SHH/GLI3 signaling. PMID: 21292980
    38. Introduction of a Gli3 mutant allele encoding the short form of Gli3 into Ftm mutants rescues both telencephalic patterning and olfactory bulb morphogenesis, despite the persistence of cilia defects. PMID: 21490064
    39. Gli3 is required for maintaining the cortical progenitors in active cell cycle, suggesting that cells may acquire differentiated status as they turn off Gli3 expression during neurogenesis. PMID: 21525285
    40. A phylogenetic analysis of the Gli3 locus that uncovered a novel regulatory element, HCNE1, is reported. PMID: 21262763
    41. GLI3 and Hh signaling controls Kit and Hcn3 expression and ureter peristalsis PMID: 21339645
    42. Data demonstrate that SuFu controls cerebellar patterning and cell differentiation in a GLI3 repressor-dependent manner. PMID: 21289193
    43. Gli3 null mice showed craniosynostosis of the lambdoid sutures and increased osteoprogenitor proliferation and differentiation. PMID: 20570969
    44. We conclude that correct Gli3 levels are particularly important for the lateral ganglionic eminence's growth, patterning, and development of axon guidance capabilities. PMID: 20943929
    45. relationship between mutation points of GLI3 & Gli3 & resulting phenotypes in humans & mice described; discussed how reduced amounts of GLI3 protein or truncated mutant GLI3 protein disrupt development of limbs, head, face [review] PMID: 20201963
    46. Data show that the species of Gli3 that accumulates at cilium tips is full-length and likely not protein kinase A phosphorylated. PMID: 20154143
    47. interaction between Gli3 and Suppressor of Fused (Sufu) regulates the formation of either repressor or activator forms of Gli3 PMID: 20360384
    48. GLI3 repressor controls nephron number by regulating ureteric tip cell expression of Wnt11 and Ret PMID: 19809516
    49. Gli2 and Gli3 collectively mediate all major aspects of Ihh function during endochondral skeletal development PMID: 19906844
    50. High-resolution mapping of the Gli3 mutation extra-toes reveals a 51.5-kb deletion. PMID: 11773971

    显示更多

    收起更多

  • 相关疾病：
    Several mutations result in developmental defects of cranofacial and limb structures. In particular the add (anterior digit-pattern deformity) and pdn (polydactyly Nagoya) alleles.
  • 亚细胞定位：
    Nucleus. Cytoplasm. Cell projection, cilium.
  • 蛋白家族：
    GLI C2H2-type zinc-finger protein family
  • 数据库链接：

    KEGG: mmu:14634

    STRING: 10090.ENSMUSP00000106137

    UniGene: Mm.5098