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  4. Recombinant Mouse Transcription factor Sp7 (Sp7)

Recombinant Mouse Transcription factor Sp7 (Sp7)

  • 中文名称：
    小鼠Sp7重组蛋白
  • 货号：
    CSB-YP823777MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Sp7重组蛋白
  • 货号：
    CSB-EP823777MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Sp7重组蛋白
  • 货号：
    CSB-EP823777MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Sp7重组蛋白
  • 货号：
    CSB-BP823777MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Sp7重组蛋白
  • 货号：
    CSB-MP823777MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Sp7
  • Uniprot No.：
  • 别名：
    Sp7; Osx; Transcription factor Sp7; C22; Zinc finger protein osterix
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-428
  • 氨基酸序列
    MASSLLEEEA HYGSSPLAML TAACSKFGGS SPLRDSTTLG KGGTKKPYAD LSAPKTMGDA YPAPFSSTNG LLSPAGSPPA PASGYANDYP PFPHSFPGPT GAQDPGLLVP KGHSSSDCLP SVYTSLDMTH PYGSWYKAGI HAGISPGPGN TPTPWWDMHP GGNWLGGGQG QGDGLQGTLS TGPAQPPLNP QLPTYPSDFA PLNPAPYPAP HLLQPGPQHV LPQDVYKPKA VGNSGQLEGS GAAKPPRGAG TGGSGGYAGS GAGRSTCDCP NCQELERLGA AAAGLRKKPI HSCHIPGCGK VYGKASHLKA HLRWHTGERP FVCNWLFCGK RFTRSDELER HVRTHTREKK FTCLLCSKRF TRSDHLSKHQ RTHGEPGPGP PPSGPKELGE GRSVGEEEAN QPPRSSTSPA PPEKAHGGSP EQSNLLEI
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Transcriptional activator essential for osteoblast differentiation. Binds to SP1 and EKLF consensus sequences and to other G/C-rich sequences.
  • 基因功能参考文献：
    1. Runt-related transcription factor 2 (Runx2) was confirmed to have synergistic effects with Osx in activating ENPP1 promoter. PMID: 30047979
    2. SIRT7 has a critical role in bone formation by regulating lysine acylation of SP7. PMID: 30026585
    3. beta-catenin in osterix-expressing cells is required for postnatal osteoblast differentiation, osteoblast proliferation, and bone resorption, and is essential for the anabolic actions of parathyroid hormone in bone. PMID: 29124436
    4. Mullerian duct(MD) regression was delayed in Osx-null males, identifying Osx as a factor that regulates MD regression. PMID: 30061417
    5. Results indicate that Dlx5 and Runx2 are critical factors for the upregulated Osterix expression in shPP2A cells, which is considered to be important for the accelerated osteoblast differentiation in these cells. PMID: 29068100
    6. Data support a model wherein p53 represses OSX-DNA binding and DLX5-OSX interaction, and thereby deregulates the osteogenic transcriptional network. PMID: 28777372
    7. this study shows that Osterix represses adipogenesis by negatively regulating PPARgamma transcriptional activity PMID: 27752121
    8. these findings strongly suggest that TGF-beta signaling plays a major role as one of the upstream regulators of Osx in cementoblast differentiation and cementum formation PMID: 27180803
    9. These results suggest proliferation and maturation of immature osteoblasts requires Tgfbr2 signaling and that decreased bone volume in Osx-Cre;Tgfbr2(fl/fl) mice is likely due to fewer mature osteoblasts. PMID: 28043895
    10. DNA damage and senescence in osteoprogenitors expressing Osx may cause their decrease with age. PMID: 28401730
    11. review of site-specific function and regulation in tooth root formation PMID: 26599722
    12. The data support a model in which Dlx recruitment of Sp7 to osteoblast enhancers underlies Sp7-directed osteoblast specification. PMID: 27134141
    13. Mmp13 is selectively regulated of by 1,25-Dihydroxyvitamin D3, PTH, and Osterix through distal enhancers. PMID: 26348136
    14. These results indicated that olfactory bulb development was not significantly impaired in the absence of Osx. PMID: 27449610
    15. Wnt3a induces Osx expression via p38 MAPK signaling in dental follicle cells. Wnt3a-induced Osx expression was inhibited in the presence of p38 mitogen-activated protein kinase (MAPK) inhibitors (SB203580 and SB202190) at gene and protein levels, as assessed by real-time PCR and immunocytohistochemistry, respectively. PMID: 27450807
    16. OSX served a key role in the development and progression of ALD-induced VSMC calcification. This observation may aid in the explanation of the role of OSX in the pathogenesis of vascular calcification PMID: 27431734
    17. results suggest that expression of Sp7 during the early stage of Satb2-induced osteogenic differentiation of BMSCs is regulated by miR-27a. PMID: 27142530
    18. Fibrillin-2 and periostin are target genes in Osterix-mediated osteoblast differentiation. PMID: 27751812
    19. osterix is a downstream target of IGF1R in chondrocytes. PMID: 26475121
    20. cells expressing osterix are mesenchymal progenitors contributing to all relevant cell types during morphogenesis. PMID: 27068606
    21. Results propose that Utx plays important roles in osteoblast differentiation by controlling the expressions of Runx2 and Osterix. PMID: 25920016
    22. Decreased expression of Osterix, as well as impaired TGFbeta and BMP2 signaling, contribute to the observed osteopenic bone phenotype of TIEG1 KO mice. PMID: 26801561
    23. Intermittent stretching promotes osteogenic differentiation of bone marrow derived mesenchymal stem cells; the p38MAPK-osterix pathway has an important role in the control of osteogenesis related gene expression. PMID: 26112248
    24. c-Src signaling modulates osteoblast differentiation at least in part through phosphorylation of Osterix. PMID: 25802190
    25. CHIP targets Osx for ubiquitination and degradation in osteoblasts after chronic exposure to TNF-alpha. PMID: 25818514
    26. conclude that OSX, one of the key downstream molecules of NFIC, plays a critical role in root, but not crown, formation PMID: 25349111
    27. Cbl-b and c-Cbl regulate the degradation of Osterix through the ubiquitin-proteasome pathway. PMID: 25744063
    28. The key role of Osx in control of cementoblast proliferation and differentiation is to maintain a low level of Wnt-beta-catenin via direct up-regulation of DKK1. PMID: 25678852
    29. These data suggest that Osx-Cre containing controls should be used for both in vivo and in vitro skeletal analyses of conditional knockout mice generated with this Osx-Cre mouse strain PMID: 25139670
    30. Pin1 regulates the osteogenic activity of Osterix. PMID: 25463757
    31. Sp7 is not required for tooth morphogenesis but is obligatory for the functional maturation of both ameloblasts and odontoblasts PMID: 25158188
    32. Nanogel-mediated delivery of Runx2 and Osx siRNA inhibits osteoblastic differentiation in murine calvarial preosteoblasts. PMID: 25448327
    33. Osx plays an important role in spine development, and its deactivation leads to severe spine deformities. PMID: 24268393
    34. The Twist2-Cre, Osterix-Cre and osteocalcin-Cre lines to generate conditional beta1 integrin deletions, were used to investigate the role of beta1 integrins on skeletal phenotype. PMID: 25183373
    35. Thyroid hormone regulates the secondary ossification center initiation and progression via differentiating chondrocytes into bone matrix-producing osteoblasts by stimulating Ihh and Osx expression in chondrocytes. PMID: 24753031
    36. findings indicate Osx is required for root formation by regulating odontoblast differentiation, maturation, and root elongation. data strongly indicate Osx is site-specific regulator in tooth root formation. PMID: 25568170
    37. Results revealed S422 as a novel phosphorylation site of Osx and GSK-3b played an important role in regulating the protein stability and transactivational activity of Osx. PMID: 25728276
    38. Osterix-expressing cells produce their own Wnts that in turn induce Wnt signaling response, thereby regulating their proliferation and differentiation PMID: 25422448
    39. Sp7/Osterix induces the mouse Col1a2 expression via the proximal promoter in osteoblastic cells in association with osteoblast differentiation. PMID: 25172663
    40. find that Osx-Cre targets not only osteoblasts, osteocytes and hypertrophic chondrocytes as expected, but also stromal cells, adipocytes and perivascular cells in the bone marrow PMID: 24454809
    41. results provide a molecular description of a mechanism for Osx and Runx2 transcriptional cooperation that is subject to further regulation by MAPK-activating signals during osteogenesis. PMID: 25122769
    42. Collectively, these data indicate that miR-143 is a novel regulator of Osx, and it might play an essential role in the regulation of osteogenic differentiation. PMID: 24381059
    43. The impact of VDR, RUNX2, and C/EBPbeta on osteoblast differentiation is exemplified by their actions at the Runx2 and Sp7 gene loci. PMID: 24891508
    44. These results uncover a broad expression profile of Osterix and raise the intriguing possibility that distinct waves of stromal cells, primitive and definitive, may organize the developing bone marrow. PMID: 24823377
    45. Osx expressed in chondrocytes regulates bone growth in part by regulating chondrocyte hypertrophy. PMID: 23943855
    46. The expression of interleukin-6, a key mediator of chronic inflammation, was increased in Osx heterozygotes and decreased in Osx overexpressing osteoblasts, and transcriptionally down-regulated by Osx. PMID: 23922826
    47. Data suggest that the hanges of Runx2 and osterix expression implied that fluoride stimulated Runx2 expression to mediate osteoblast differentiation. PMID: 23892695
    48. Jmjd3 plays important roles in osteoblast differentiation and regulates the expressions of BSP and OCN via transcription factors Runx2 and osterix. PMID: 24106268
    49. there is a tight correlation between Osx and the miRNAs involved in bone formation PMID: 23472141
    50. The spacial expression pattern and functional significance Sox9, Runx2, and Osterix in cartilages during the late development of mouse mandible, were investigated. PMID: 23762831

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  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    Sp1 C2H2-type zinc-finger protein family
  • 组织特异性：
    Osteoblast/chondrocyte specific.
  • 数据库链接：

    KEGG: mmu:170574

    STRING: 10090.ENSMUSP00000077596

    UniGene: Mm.263284