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  4. Recombinant Mouse Transcription factor E2F4 (E2f4)

Recombinant Mouse Transcription factor E2F4 (E2f4)

  • 中文名称：
    小鼠E2f4重组蛋白
  • 货号：
    CSB-YP848090MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠E2f4重组蛋白
  • 货号：
    CSB-EP848090MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠E2f4重组蛋白
  • 货号：
    CSB-EP848090MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠E2f4重组蛋白
  • 货号：
    CSB-BP848090MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠E2f4重组蛋白
  • 货号：
    CSB-MP848090MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    E2f4Transcription factor E2F4; E2F-4
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-410
  • 氨基酸序列
    AEAGPQAPP PPGTPSRHEK SLGLLTTKFV SLLQEAKDGV LDLKLAADTL AVRQKRRIYD ITNVLEGIGL IEKKSKNSIQ WKGVGPGCNT REIADKLIEL KAEIEELQQR EQELDQHKVW VQQSIRNVTE DVQNSCLAYV THEDICRCFA GDTLLAIRAP SGTSLEVPIP EGLNGQKKYQ IHLKSMSGPI EVLLVNKEAW SSPPVAVPVP PPDDLLQSPP AVSTPPPLPK PALAQPQESS PPSSPQLTTP TPVLGSTQVS EVACQTSEIA VSGSPGTENK DSGEVSSLPL GLTALDTRPL QSSALLDSSS SSSSSSSSSS SSSSGPNPST SFEPIKADPT GVLDLPKELS EIFDPTRECM SSELLEELMS SEVFAPLLRL SPPPGDHDYI YNLDESEGVC DLFDVPVLKL
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F4 binds with high affinity to RBL1 and RBL2. In some instances can also bind RB1. Specifically required for multiciliate cell differentiation: together with MCIDAS and E2F5, binds and activate genes required for centriole biogenesis.
  • 基因功能参考文献：
    1. E2f4 forms apical cytoplasmic organizing centres for assembly and nucleation of deuterosomes. Using genetically altered mice and E2F4 mutant proteins we demonstrate that centriole amplification is crucially dependent on these organizing centres and that, without cytoplasmic E2f4, deuterosomes are not assembled, halting multiciliogenesis PMID: 28675157
    2. E2f4 and E2f5 display overlapping roles in controlling the normal development of the male reproductive system. PMID: 26825228
    3. Following stress exposure, E2F4-p130 complexes are lost rapidly along with the presence of E2F4 at E2F-containing B-Myb promoter sites. PMID: 24828495
    4. E2F4 is part of a transcriptional repressor complex comprising p130 and HDAC1 in cardiac myocytes. Relief from E2F4-dependent gene silencing induces the expression of pro-apoptotic genes. PMID: 22985930
    5. In terminally differentiated cells, common KDM5A and E2F4 gene targets were bound by the pRB-related protein p130, a DREAM complex component. PMID: 23093672
    6. We report here a new cellular function of p27 as a transcriptional regulator in association with p130/E2F4 complexes that could be relevant for tumorigenesis. PMID: 22179826
    7. Induction of Wnt/beta-catenin signaling in mouse mesenchymal stem cells is associated with activation of the p130 and E2f4 and formation of the p130/Gsk3beta/beta-catenin complex. PMID: 21631154
    8. E2F4 plays an important role in enabling osteoblast progenitors to exit the cell cycle and subsequently differentiate thereby contributing to the commitment of these cells to the bone lineage. PMID: 20581455
    9. Our data indicate that E2F4 is required for cardiomyocyte proliferation and suggest a function for E2F4 in mitosis PMID: 19955219
    10. E2F4 controls cell cycle exit through different mechanisms. PMID: 20023434
    11. interactions are regulated by G1 cyclin/cyclin-dependent kinase-coordinated phosphorylation of endogenous pocket proteins PMID: 12401786
    12. E2F4 has a role in adipogenesis independent of its cell cycle regulatory activity PMID: 12604789
    13. E2F3a and E2F4, but not E2F5, function to induce cell cycle entry, although E2F4 has more modest activity. E2F3a may induce cell death primarily through activation of p73alpha. PMID: 15855772
    14. macrocytic anemia of E2f4(-/-) mice results primarily from impaired cellular proliferation and that the major role of E2f4 in fetal erythropoiesis is to promote cell cycle progression and cellular proliferation. PMID: 16861343
    15. Required to determine the appropriate development of the airway epithelium. PMID: 17383628
    16. This study investigated if the distribution between nucleus and cytoplasm of the transcription factors E2F4 and E2F5 is mediated by pRb2/p130 and if the presence of JCV T-antigen may impair this shuttling by sequestering pRb2/p130. PMID: 17385710
    17. A novel requirement is established for cell cycle regulatory protein E2F4 in the development of the ventral telencephalon. PMID: 17537963
    18. The E2F transcription factors are key cell cycle regulators that play important roles in modulating cell proliferation and cell fate. PMID: 17652516
    19. normal growth-arrested enterocytes contain p130-E2F4 complexes and T-antigen expression of SV40 destroys these complexes PMID: 17855529
    20. Cell cycle genes are the evolutionarily conserved targets of the E2F4 transcription factor PMID: 17957245
    21. a subset of the Rb(-/-);E2f4(-/-) chimeras developed either low- or high-grade carcinomas in the urothelium transitional epithelium supporting a key role for Rb in bladder cancer. PMID: 18997819
    22. E2F1 expression remained almost unchanged at all stages of chondrocyte differentiation, whereas E2F4 expression increased markedly at the late stage of differentiation. PMID: 19142862
    23. the cell cycle repressor E2F4 is essential for the formation of functional memory T cells. A decrease in CD8+ T-lymphocyte compartment would diminish our capacity to control viral infections. PMID: 19306992
    24. the results of our studies show a novel role of E2F4 in the early eye development. PMID: 19325228
    25. pRB and E2F4 cooperate in placental development, but play largely non-overlapping roles in the development of many embryonic tissues. PMID: 19433082
    26. provide evidence that alternative reading frame reduces the interaction of the E2F4-p130 repressor complex with the promoter of xeroderma pigmentosum, complementation group C (XPC) to ensure high-level expression of XPC. PMID: 19644500
    27. The absence of E2F4 delayed Myc-induced lymphomagenesis. PMID: 19749980

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  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    E2F/DP family
  • 数据库链接：

    KEGG: mmu:104394

    STRING: 10090.ENSMUSP00000015003

    UniGene: Mm.34554