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  4. Recombinant Mouse Tissue-type plasminogen activator (Plat)

Recombinant Mouse Tissue-type plasminogen activator (Plat)

  • 货号：
    CSB-YP018120MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP018120MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP018120MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP018120MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP018120MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Plat; Tissue-type plasminogen activator; t-PA; t-plasminogen activator; tPA; EC 3.4.21.68) [Cleaved into: Tissue-type plasminogen activator chain A; Tissue-type plasminogen activator chain B]
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    33-559
  • 氨基酸序列
    SYRATCRD EPTQTTYQQH QSWLRPMLRS SRVEYCRCNS GLVQCHSVPV RSCSEPRCFN GGTCQQALYF SDFVCQCPDG FVGKRCDIDT RATCFEEQGI TYRGTWSTAE SGAECINWNS SVLSLKPYNA RRPNAIKLGL GNHNYCRNPD RDLKPWCYVF KAGKYTTEFC STPACPKGKS EDCYVGKGVT YRGTHSLTTS QASCLPWNSI VLMGKSYTAW RTNSQALGLG RHNYCRNPDG DARPWCHVMK DRKLTWEYCD MSPCSTCGLR QYKRPQFRIK GGLYTDITSH PWQAAIFVKN KRSPGERFLC GGVLISSCWV LSAAHCFLER FPPNHLKVVL GRTYRVVPGE EEQTFEIEKY IVHEEFDDDT YDNDIALLQL RSQSKQCAQE SSSVGTACLP DPNLQLPDWT ECELSGYGKH EASSPFFSDR LKEAHVRLYP SSRCTSQHLF NKTVTNNMLC AGDTRSGGNQ DLHDACQGDS GGPLVCMINK QMTLTGIISW GLGCGQKDVP GVYTKVTNYL DWIHDNMKQ
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Converts the abundant, but inactive, zymogen plasminogen to plasmin by hydrolyzing a single Arg-Val bond in plasminogen. By controlling plasmin-mediated proteolysis, it plays an important role in tissue remodeling and degradation, in cell migration and many other physiopathological events. During oocyte activation, plays a role in cortical granule reaction in the zona reaction, which contributes to the block to polyspermy.
  • 基因功能参考文献：
    1. Sata suggest that tissue-type plasminogen activator (tPA) regulates nocturnal wheel-running activity, and that tPA differentially affects suprachiasmatic nucleus-driven nocturnal activity rhythms and activity driven by fasting or temporal food restriction. PMID: 29370799
    2. Our findings suggest that isoflurane pretreatment could attenuate tPA-exaggerated brain ischemic injury, by reducing tPA-induced LRP/NF-kappaB/Cox-2 in endothelial cells, endothelial MMP-2 and MMP-9 activation, and subsequent pro-apoptotic molecule in neurons after oxygen/glucose deprivation and reperfusion. PMID: 28539818
    3. Tissue plasminogen activator was found to be restricted to subsets of excitatory pyramidal glutamatergic neurons. PMID: 26377106
    4. analysis of mutations in genes co-segregating with the targeted Plat allele, including several potential null mutations PMID: 27079292
    5. By mediating the tPA response in macrophages, the NMDA-R provides a pathway by which the fibrinolysis system may regulate innate immunity. PMID: 28684538
    6. Altogether, this preclinical study demonstrates that the tPA present in the blood stream is a key player of the formation of intracranial aneurysms. PMID: 28754830
    7. A model of sustained hyperfibrinolytic state can be obtained in mice by hydrodynamic transfection of a plasmid encoding for tissue-type plasminogen activator (tPA). PMID: 27531677
    8. While the absence of t-PA confers a more deleterious form of Experimental Autoimmune Encephalomyelitis, neuronal over-expression of t-PA does not overtly protect against this condition with regards to symptom onset or severity of Experimental Autoimmune Encephalomyelitis. PMID: 27427941
    9. mesenchymal stromal cells promote axonal outgrowth via neuronal tPA and synergistically with astrocytic tPA PMID: 27959956
    10. administration of ADAMTS13 5 minutes after occlusion dose-dependently dissolved these t-PA-resistant thrombi resulting in fast restoration of MCA patency and consequently reduced cerebral infarct sizes PMID: 26929275
    11. strategies contributing to promote either endogenous production of tPA or its associated EGFR-linked signaling pathway may have beneficial effects following brain injuries such as stroke. PMID: 26068590
    12. Plasminogen activation contributes to wound repair and suggest that adenosine A2A receptor activation promotes wound closure by a mechanism that depends on tPA. PMID: 25991438
    13. Myeloid-derived Tissue-type plasminogen activator (tPA) promotes macrophage migration through a novel signaling cascade involving FAK, Rac-1, and NF-kappaB. PMID: 25131752
    14. the protective effect of neuroserpin maybe independent from its canonical interaction with a tissue-type plasminogen activator PMID: 26176694
    15. Erythropoietin protects mouse renal tubular basement membrane by promoting bone marrow cells to generate and secrete miR-144, which, in turn, inhibits activation of the tPA/MMP9-mediated proteolytic network. PMID: 26469975
    16. tPA activity in V1 can be unmasked following 4 d of monocular deprivation when mice older than 2 months are raised in standard cages by the genetic removal of Lynx1, a negative regulator of adult plasticity. PMID: 26377459
    17. Results demonstrate that synaptic potentiation results in tissue plasminogen activator-dependent reelin processing and suggest that extracellular proteolysis of reelin may regulate reelin signaling in the adult brain PMID: 24892761
    18. data implicate MCP-1 as the key molecule governing tPA-induced fluid accumulation. The role of MCP-1 in the development of other exudative effusions warrants examination. PMID: 25474480
    19. Data indicated that suggest that tPA modulates emotion in a social context through the function of the prefrontal cortex. PMID: 25499620
    20. in vivo and in vitro evidence showing that this aggravation is correlated with a decrease in lipoprotein receptor-related protein 1-mediated hepatic clearance of tPA in alcohol-exposed mice. PMID: 25922513
    21. Data indicate that closed head trauma sequentially releases tissue-type plasminogen activator (tPA) followed by delayed synthesis and release of urokinase plasminogen activator (uPA) from injured brain. PMID: 25673638
    22. tPA promoted M1 macrophage survival through its receptor LRP-1-mediated novel signaling cascade involving Erk1/2, p90RSK, and p38, which leads to the accumulation of these cells in the injured kidneys PMID: 25670857
    23. gene deficiency results in aggravation of experimental autoimmune myasthenia gravis PMID: 24423642
    24. plg-/- and tPA-/- mice exhibit brain parenchymal fibrin deposits that appear to result from reduced neurovascular integrity PMID: 24612416
    25. Neural progenitor cells from tPA knock-out mice show impaired neurite outgrowth compared to wild-type. PMID: 23925701
    26. Data suggest that tPA is required for bone repair and osteoblast proliferation; bone repair of the femur is delayed in tPA knockout mice, unlike that in wild-type mice (or uPA [urokinase-type plasminogen activator] knockout/wild-type mice). PMID: 24918201
    27. Endogenously released tPA promotes neutrophil transmigration to reperfused tissue via proteolytic activation of plasmin and gelatinases. PMID: 24764453
    28. TPA played an essential role in augmenting circulating endothelial progenitor cells, angiogenesis, and blood flow in the ischemic limb in a murine model. PMID: 24290069
    29. tPA is sufficient to induce interleukin-1beta and phosphorylated extracellular signal-regulated kinase (ERK) expression in astrocyte cultures. PMID: 23707980
    30. excess tPA/plasmin, through separate downstream molecular mechanisms, regulates postnatal Purkinje neuron (PN) dendritogenesis, synaptogenesis, mitochondrial structure and function, and selective PN viability PMID: 23674688
    31. Our data indicate that tPA turns on a cell signaling pathway that protects neurons from the deleterious effects of excitotoxicity. PMID: 23063501
    32. Chronic phase shifts resulted in decreased tissue-type plasminogen activator mRNA expression in the liver. PMID: 23249568
    33. The serine protease tissue plasminogen activator (tPA) and two matrix metalloproteinases, ADAMTS-4 and ADAMTS-5, were identified as Reelin cleaving enzymes. PMID: 23082219
    34. Altogether, our data provide the first evidence that tPA influences spatial memory through its preferential interaction with GluN2D subunit-containing NMDARs. PMID: 22972996
    35. activates the NF-kappaB pathway in macrophages through a signaling pathway involving annexin A2/CD11b-mediated integrin-linked kinase PMID: 22677557
    36. This study provides new evidences supporting that tPA plays a cytokine-like role in glial activation by triggering receptor-mediated intracellular signaling circuits. PMID: 22162045
    37. This study finding showed that the tPA/plasmin cascade may act downstream of ChABC to allow for synergistic sensory and motor improvement compared with each treatment alone and suggest a potential new approach to enhance functional recovery after SCI. PMID: 22016526
    38. role in the nasal mucosa reconstruction in allergic rhinitis PMID: 21339035
    39. synthetic nonadecapeptide binds to the B region and promotes the activation of plasminogen by t-PA, and then induced effective thrombolysis PMID: 21392255
    40. tPA protects white matter from stroke-induced lesions PMID: 21576385
    41. Voluntary physical activity, but not locked wheel exposure, induced a robust increase in hippocampal mature BDNF protein levels, as well as in p11 and tPA mRNA expression; and also promoted antidepressive effects and improved learning PMID: 21371535
    42. phosphodiesterase-III inhibitor prevents the hemorrhagic transformation induced by focal cerebral ischemia in mice treated with tPA PMID: 21151895
    43. Tissue plasminogen activator is required for the development of fetal alcohol syndrome in mice PMID: 21383198
    44. tPA activity is regulated reversibly in an experience-dependent manner in the adult hippocampus PMID: 20974200
    45. Data indicate that the neuroprotective effect of neuroserpin is due to inhibition of plasmin-mediated excitotoxin-induced cell death and is independent of neuroserpin's ability to inhibit tissue plasminogen activator activity. PMID: 20864675
    46. Both tissue-type PA (tPA) and urokinase-type PA (uPA) bind to NMDA-R1 and reverse this effect, thereby enhancing acetylcholine-induced tracheal contractility. PMID: 20097831
    47. these results demonstrate a new function for tPA in the central nervous system, which most likely contributes to its pleiotropic functions. PMID: 20636282
    48. Data suggest that the MSC mediated increased activation of tPA in astrocytes promotes neurite outgrowth after stroke. PMID: 20140248
    49. We suggest that AVP secretion is critically regulated by tPA-dependent facilitation of AVP release from terminals and plasminogen-dependent increase of AVP permeability across microvessels possibly via laminin degradation. PMID: 20175210
    50. Plasmin and its activators tPA and uPA play a role in the haematopoietic recovery. PMID: 19210287

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  • 亚细胞定位：
    Secreted, extracellular space.
  • 蛋白家族：
    Peptidase S1 family
  • 数据库链接：

    KEGG: mmu:18791

    STRING: 10090.ENSMUSP00000033941

    UniGene: Mm.154660