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  4. Recombinant Mouse Thioredoxin (Txn)

Recombinant Mouse Thioredoxin (Txn)

  • 货号：
    CSB-YP025365MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP025365MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP025365MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP025365MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP025365MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Txn; Txn1; Thioredoxin; Trx; ATL-derived factor; ADF
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-105
  • 氨基酸序列
    VKLIESKEA FQEALAAAGD KLVVVDFSAT WCGPCKMIKP FFHSLCDKYS NVVFLEVDVD DCQDVAADCE VKCMPTFQFY KKGQKVGEFS GANKEKLEAS ITEYA
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions. Plays a role in the reversible S-nitrosylation of cysteine residues in target proteins, and thereby contributes to the response to intracellular nitric oxide. Nitrosylates the active site Cys of CASP3 in response to nitric oxide (NO), and thereby inhibits caspase-3 activity. Induces the FOS/JUN AP-1 DNA binding activity in ionizing radiation (IR) cells through its oxidation/reduction status and stimulates AP-1 transcriptional activity.; ADF augments the expression of the interleukin-2 receptor TAC (IL2R/P55).
  • 基因功能参考文献：
    1. Study in transgenic mice finds that T-cell activation is linked to a drastic downregulation of Txnip and an increase in Txn1/Txnrd1 expression, which is absolutely required for synthesis of 2'-deoxyribonucleotides during T-cell metabolic reprogramming. These results uncover a pivotal function of the Trx1 system in metabolic reprogramming of thymic and peripheral T cells. PMID: 29749372
    2. Up-regulation thioredoxin resulted in decreased intracellular ROS generation and the underlying mechanism is related to inhibition of the Txnip/mTOR pathway mediated autophagy. PMID: 30384364
    3. mTOR is a direct interacting partner of Trx1 in the heart. Trx1 protects cardiomyocytes against oxidative stress by reducing mTOR at Cys-1483, thereby preserving the activity of mTOR and inhibiting cell death. PMID: 28939765
    4. By establishing that IL-4 is posttranslationally regulated by TRX-promoted reduction of a disulfide bond, our findings highlight a novel regulatory mechanism of the type 2 immune response that is specific to IL-4 over IL-13. PMID: 30104382
    5. Nox2 activation favors thioredoxin-1 (TRX-1)/p40phox interaction, which leads to exclusion of TRX-1 from the nucleus. PMID: 27698473
    6. These results suggest that overexpression of Trx-1 may occlude the CPP induced by METH through regulating the activity of CREB and the expression of DeltaFosB. PMID: 28782589
    7. A novel mouse model for the identification of thioredoxin-1 protein interactions has been created. PMID: 27639450
    8. Serum thioredoxin reductase is highly increased in mice with hepatocellular carcinoma and its activity is restrained by several mechanisms, in particular, by the serum QSOX1. PMID: 27581528
    9. Nuclear overexpression of Trx1 restored Nrf2 activity and attenuated alcohol-induced acute lung injury. PMID: 27436123
    10. Trx1 enhances blood perfusion and increases angiogenic protein expression in a rodent hind limb ischemia model. PMID: 28807201
    11. GSR is not essential for the maintenance of antioxidant defenses in mouse cochlea; the thioredoxin/thioredoxin reductase system can probably operate as a functional backup for GSR. PMID: 28686716
    12. These results strongly suggest that Trx1 ameliorates the myocardial effects of I/R by improving the free radical-mediated damage in cardiac and mitochondrial function, opening the possibility of new therapeutic strategies in coronary artery disease. PMID: 27000416
    13. Thioredoxin-mediated deglutathionylation of eNOS in the coronary artery in vivo protected against reperfusion injury, even in the presence of normal levels of glutathione. PMID: 27587398
    14. Acute stimuli of epinephrine induced Trx-1 expression through activating CREB. PMID: 27511023
    15. results suggest that PostC prevents Trx-1 degradation, decreasing oxidative stress and allowing the activation of Akt and GSK3beta to exert its cardioprotective effect PMID: 26932791
    16. GL-V9 attenuated DSS-induced colitis against oxidative stress by up-regulating Trx-1 via activation of AMPK/FOXO3a pathway. PMID: 26327408
    17. The ventral signal observed from E12.5 onward colocalized with motor neuron markers Isl1/2 and FoxP1. and the strong ventral signal colocalizing with Isl1/2 was observed in all rostrocaudal segments of the spinal cord PMID: 25975898
    18. the timely upregulation of Trx1/TrxR1 and the active control of intracellular redox status is critical for the survival of thymocytes during and short after positive selection. PMID: 25753394
    19. Trx1 may also modulate lysine methylation, a non-redox post-translational modification, via the regulation of SMYD1 expression PMID: 26410624
    20. Translocation of Trx1 from cytoplasm to cell nuclei plays an important role in disturbed flow-stimulated proatherogenesis with greater cytoplasmic protein oxidation and an enhanced nuclear transcription factor activity. PMID: 25265386
    21. Our results indicate a close relationship between Trx1 and the 20S nuclear proteasome PMID: 23850970
    22. Data indicate that Trx-1 mediated endoplasmic reticulum and mitochondria pathways are involved in brain damage in myasthenia gravis PMID: 24878227
    23. Suggest that urinary TRX1 is an oxidative stress-specific biomarker useful for distinguishing AKI from chronic kidney disease and healthy kidneys. PMID: 25350977
    24. Thioredoxin-1 expression suppresses endoplasmic reticulum stress in a mouse model of Parkinson disease. PMID: 24140863
    25. Therefore, we conclude that TIGAR knockdown-induced radiosensitization of glioma cells may be dependent on the inhibition of TRX1 nuclear translocation. PMID: 24509157
    26. Thioredoxin-1 enhanced pulmonary mRNA expression of MAP kinase phosphatase 1 (MKP-1), and the suppressive effects of thioredoxin-1 on prolonged GM-CSF release and late neutrophilic inflammation disappeared by inhibiting MKP-1. PMID: 24244404
    27. Thioredoxin-1 functions as a molecular switch to turn off the oxidative stress-induced activation of MST1. PMID: 23085515
    28. TR1-deficient cells are more sensitive to TNF-alpha induced apoptosis than control cells. PMID: 24039713
    29. Inhibition of Trx activity in the lung attenuates LPS-induced SNO-p65 denitrosylation, NF-kappaB activation, and airway inflammation, supporting a pathophysiological role for this mechanism in lung injury. PMID: 24338024
    30. Thioredoxin-1 attenuates early graft loss after intraportal islet transplantation in mice. PMID: 23950917
    31. Data indicate that thioredoxin was among the 32 proteins that displayed greater than a twofold increase in spectral counts in the bone marrow (BM) supernatant of AMD3100-treated transplant mice. PMID: 23994289
    32. These results indicate that Trx functions as a physiological inhibitor of MPK38, which plays an important role in inducing ASK1-, TGF-beta-, and p53-mediated activity. PMID: 23747528
    33. This study identifies Trx-1 as a novel interaction partner of the ADAM17 cytoplasmic domain. PMID: 23105116
    34. Study identifies a role for Trx1 and its association with its regulatory proteins TXNIP, AKAP12, and subsequent activation of Akt/GSK-3beta/beta-catenin/HIF-1alpha-mediated VEGF and eNOS expression in inducing angiogenesis and reduced ventricular remodeling. PMID: 21074540
    35. S. zooepidemicus uses SzP to recruit TRX and regulated the alternative complement pathways to evade the host immune phagocytosis PMID: 22384152
    36. Thioredoxin-1 regulates cellular heme insertion by controlling S-nitrosation of glyceraldehyde-3-phosphate dehydrogenase. PMID: 22457359
    37. male Tg(TRX1)(+/0) mice significantly extended the earlier part of life span compared with wild-type littermates, but no significant life extension was observed in females PMID: 21873593
    38. Trx associates with the active site of 3-mercaptopyruvate sulfurtransferase to release hydrogen sulfide in brain mitochondria in vitro. PMID: 21732914
    39. Thioredoxin 1 negatively regulates angiotensin II-induced cardiac hypertrophy through upregulation of miR-98/let-7. PMID: 21183740
    40. DJ-1 has anti-oxidant and cytoprotective properties by regulating the thioredoxin 1 ASK1 complex and controlling the availability of ASK1 to effect apoptosis PMID: 20385180
    41. TR1 and Trx1 interaction play key role in redox homoeostasis. PMID: 20536427
    42. The roles of thioredoxin in protection against oxidative stress-induced apoptosis PMID: 11751890
    43. Th1/Th2 balance is polarizated to Th1 in thioredoxin (TRX) transgenic mice during aging PMID: 11841834
    44. In addition to an antioxidant effect, Trx has the potential to protect acute liver injury via an antiapoptotic effect, which mainly inhibits mitochondria-mediated apoptosis signaling. PMID: 12717382
    45. increased levels of Trx-1 in human tumors could lead to functional inhibition of PTEN tumor suppressor activity providing an additional mechanism for tumorigenesis with loss of PTEN activity PMID: 15313215
    46. critical role for the LIMK family in the regulation of ADF/cofilin and synaptic function in the brain. PMID: 15458846
    47. The present study suggests that TRX-1 has potent antioxidant and anti-inflammatory actions in experimental acute pancreatitis and might be a new therapeutic strategy to improve the prognosis of severe acute pancreatitis. PMID: 16322089
    48. increased ROS production in aged liver may alter the ratio of ASK1 and Trx-ASK1, thereby increasing the age-associated basal level of p38 MAPK pathway activity PMID: 16449798
    49. results suggest that thioredoxin-1 may be a key signaling molecule regulating the early events in the IgE signaling in mast cells and allergic inflammation PMID: 16474438
    50. The results suggest that, in cardiac myocytes, Trx1 upregulates mitochondrial proteins and enhances mitochondrial functions, possibly through PGC-1alpha and NRFs. PMID: 16987018

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  • 亚细胞定位：
    Nucleus. Cytoplasm. Secreted.
  • 蛋白家族：
    Thioredoxin family
  • 数据库链接：

    KEGG: mmu:22166

    STRING: 10090.ENSMUSP00000030051

    UniGene: Mm.260618