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  4. Recombinant Mouse TNF receptor-associated factor 2 (Traf2)

Recombinant Mouse TNF receptor-associated factor 2 (Traf2)

  • 货号：
    CSB-YP024147MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP024147MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP024147MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP024147MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP024147MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Traf2; TNF receptor-associated factor 2; EC 2.3.2.27; E3 ubiquitin-protein ligase TRAF2; RING-type E3 ubiquitin transferase TRAF2
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-501
  • 氨基酸序列
    AAASVTSPG SLELLQPGFS KTLLGTRLEA KYLCSACKNI LRRPFQAQCG HRYCSFCLTS ILSSGPQNCA ACVYEGLYEE GISILESSSA FPDNAARREV ESLPAVCPND GCTWKGTLKE YESCHEGLCP FLLTECPACK GLVRLSEKEH HTEQECPKRS LSCQHCRAPC SHVDLEVHYE VCPKFPLTCD GCGKKKIPRE TFQDHVRACS KCRVLCRFHT VGCSEMVETE NLQDHELQRL REHLALLLSS FLEAQASPGT LNQVGPELLQ RCQILEQKIA TFENIVCVLN REVERVAVTA EACSRQHRLD QDKIEALSNK VQQLERSIGL KDLAMADLEQ KVSELEVSTY DGVFIWKISD FTRKRQEAVA GRTPAIFSPA FYTSRYGYKM CLRVYLNGDG TGRGTHLSLF FVVMKGPNDA LLQWPFNQKV TLMLLDHNNR EHVIDAFRPD VTSSSFQRPV SDMNIASGCP LFCPVSKMEA KNSYVRDDAI FIKAIVDLTG L
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Regulates activation of NF-kappa-B and JNK and plays a central role in the regulation of cell survival and apoptosis. Required for normal antibody isotype switching from IgM to IgG. Has E3 ubiquitin-protein ligase activity and promotes 'Lys-63'-linked ubiquitination of target proteins, such as BIRC3, RIPK1 and TICAM1. Is an essential constituent of several E3 ubiquitin-protein ligase complexes, where it promotes the ubiquitination of target proteins by bringing them into contact with other E3 ubiquitin ligases. Regulates BIRC2 and BIRC3 protein levels by inhibiting their autoubiquitination and subsequent degradation; this does not depend on the TRAF2 RING-type zinc finger domain. Isoform 2 does not seem to mediate activation of NF-kappa-B but inhibits isoform 1 activity. Plays a role in mediating activation of NF-kappa-B by EIF2AK2/PKR. In complex with BIRC2 or BIRC3, promotes ubiquitination of IKBKE.
  • 基因功能参考文献：
    1. Intestinal I/R induced the membrane translocation and phosphorylation of PKCzeta. Pretreatment with the PKCzeta activator phosphatidylcholine remarkably attenuated gut injury by suppressing apoptosis. H/R induced PKCzeta to combine with TRAF2, which was phosphorylated by PKCzeta at Ser(55), but not at Ser(11), under intestinal I/R or H/R conditions PMID: 28726782
    2. work reveals that simulated microgravity promotes the apoptotic response through a combined modulation of the Uev1A/TICAM/TRAF/NF-kappaB-regulated apoptosis and the p53/PCNA- and ATM/ATR-Chk1/2-controlled DNA-damage response pathways. PMID: 26887372
    3. Traf2 mediates the pro-survival pathway in the heart by suppressing necroptotic signaling. PMID: 28572508
    4. Targeting TRAF2 may be useful as a therapeutic approach for immunosuppression-free islet allograft survival that avoids the thromboembolic complications associated with the use of anti-CD40L antibodies. PMID: 28062921
    5. Celastrol promotes Nur77 migration from the nucleus to mitochondria, where it is ubiquitinated by TRAF2. Ubiquitinated Nur77 then interacts with p62/SQSTM1, leading to autophagy of dysfunctional mitochondria and alleviation of inflammation. PMID: 28388439
    6. TRAF2 and OTUD7B govern a ubiquitin-dependent switch that regulates mTORC2 signalling PMID: 28489822
    7. this study shows that TRAF2 and TRAF5 work as important regulators of the IL-6R signaling needed for Th17 development PMID: 27076680
    8. TRAF2 functions as a key activator of MST1 in oxidative stress-induced intracellular signaling processes. PMID: 26698664
    9. Proinflammatory TLR signalling is regulated by a TRAF2-dependent proteolysis mechanism in macrophages. PMID: 25565375
    10. These studies demonstrate a critical role for TRAF2 in the maintenance of peripheral CD8(+) CD44(hi) CD122(+) T-cell and NKT-cell homeostasis by modulating sensitivity to T-cell intrinsic growth factors such as IL-15. PMID: 25931426
    11. HGK/MAP4K4 deficiency has a role in inducing TRAF2 stabilization and Th17 differentiation leading to insulin resistance PMID: 25098764
    12. identifying the CYLD-TRAF2-p38MAPK pathway as a novel important regulator of HSC function restricting HSC cycling and promoting dormancy. PMID: 25824820
    13. Data indicate that caspase-8 activity is lost upon deletion of c-FLIPL, and p43FLIP rescues caspase-8 activity through Raf1, TRAF2, and RIPK1 association, augmenting ERK and NF-kappaB pathways PMID: 24275659
    14. IpaH0722 dampens the acute inflammatory response by preferentially inhibiting the PKC-mediated activation of NF-kappaB by ubiquitinating TRAF2, a molecule downstream of PKC, and by promoting its proteasome-dependent degradation. PMID: 23754945
    15. Expression of endogenous TRAF2 is negligible in Traf2DN-transgenic B cells compared with wild-type mice. PMID: 22711886
    16. hepatic TRAF2 cell autonomously promotes hepatic gluconeogenesis by enhancing the hyperglycemic response to glucagon. PMID: 22315325
    17. Sex differences affected the response of myocardial tissues in dealing with cardiac endoplasmic reticulum stress in part through the regulation of SERCA2, CHOP, Ire1alpha and TRAF2. PMID: 20850991
    18. TRAF2 plays a crucial role in controlling homeostasis of the colon. PMID: 21393251
    19. Data show that MCPIP1 negatively regulates c-Jun N-terminal kinase (JNK) and NF-kappaB activity by removing ubiquitin moieties from proteins, including TRAF2, TRAF3, and TRAF6. PMID: 21115689
    20. Traf2- and Nck-interacting kinase is essential for Wnt signaling and colorectal cancer growth. PMID: 20530691
    21. Hence, our data show that the direct interaction between TRAF2 and MLK3 is required for TNF-alpha-induced activation of MLK3 and its downstream target, JNK. PMID: 19918265
    22. TRAF2 is required for the activation of CD120b-mediated IgM secretion by B cells. PMID: 11907088
    23. Regulation of TRAF2 signaling by self-induced degradation PMID: 11909853
    24. Dominant negative gene inhibited activation of NF-kappa B, which was also inhibited in embryonic fibroblasts derived from null mice and mediated JNK activation in response to endoplasmic reticulum stress PMID: 12021180
    25. TNFR-associated factor-2 binding site is critical for synergistic effects of CD40 and BCR signals. PMID: 12133933
    26. An essential role of TRAF2-linked receptors is shown in secondary CD4 and CD8 T cell responses that has important implications for transplantation. PMID: 12218092
    27. CD40 transgenes lacking specific binding sites were used to define the role of TRAF proteins in Ig class switiching. Binding to TRAF2 and/or TRAF3 but not TRAF6 is essential for CD40 isotype switching and activation in B cells. PMID: 12354380
    28. Regulation of the subcellular localization by TRAF1 reveals the mechanism of signaling. PMID: 12370254
    29. investigation as a target of Siah2 for ubiquitylation and degradation in vitro PMID: 12411493
    30. TRAF2 has a role in TNF-alpha induced IKK phosphorylation of NF-kappaB p65 PMID: 12842894
    31. TRAF2 exerts its antiapoptotic effect by regulating the expression of Kruppel-like factor LKLF PMID: 12897154
    32. TRAF2 has roles in CD40 signaling in B lymphocytes PMID: 12958312
    33. TRAF2 is involved in CD40 signaling PMID: 12960157
    34. TRAF2 appears to be a critical adapter molecule that is required for both OX40-enhanced early T cell proliferation and the eventual conversion of these cells into long-lived memory T cells. PMID: 14634111
    35. RIP, TRAF2, and FADD are crucial in mediating ROS accumulation in TNF-induced necrotic cell death PMID: 14701813
    36. role of RIP and TRAF2 and lipid rafts in the transduction of cell death signal initiated by H(2)O(2); activation of c-jnk1 is a critical event downstream of RIP and TRAF2 in mediating ROS-induced cell death PMID: 15199146
    37. TRAF2 is a multifunctional regulator of NF-kappaB activation that mediates activation of the canonical pathway but acts as a negative regulator of the noncanonical pathway. PMID: 15539150
    38. TRAF2DN/Bcl-2 double-transgenic mice developed small B cell lymphoma progressing to leukemia PMID: 15545599
    39. role of TRAF2 in regulating endoplasmic reticulum stress responses and the role of NF-kappaB in protecting cells from ER stress-induced apoptosis PMID: 16299380
    40. Poly(ADP-ribose)polymerase-1 signaling to mitochondria in necrotic cell death requires Traf2-mediated JNK1. PMID: 16446354
    41. an LMP1-associated complex containing TRAF6, TAB2, and TAK1 plays an essential role in the activation of JNK PMID: 16446357
    42. role for the TNFR1-TRAF2-RelA-dependent canonical pathway in the development of Peyer's patches through up-regulation of cxcl13 mRNA. PMID: 17277132
    43. The active caspase-8c-FLIP complex forms in the absence of Fas (CD95/APO1) and associates with the NF-kappaB signaling molecules RIP1, TRAF2, and TRAF6, as well as upstream NF-kappaB regulators PKC theta, CARMA1, Bcl-10, and MALT1 PMID: 17462996
    44. TRAF2 and TRAF3 were shown to function in a cooperative and nonredundant manner to suppress nuclear factor-kappaB2 (NF-kappaB2) activation, gene expression, and survival in mature B cells. PMID: 18313334
    45. OIP-1 decreased (3.5-fold) measles virus nucleocapsid protein induced TRAF2 expression during osteoclast differentiation PMID: 18348201
    46. place TRAF2 directly in the signaling pathway transduced through the IFNAR1 subunit of the IFN receptor PMID: 18362156
    47. Lysosomal degradation of cIAP1-TRAF2 by TWEAK/FN14 therefore critically alters the balance of life/death signals emanating from TNF-R1 in immortalized cells. PMID: 18606850
    48. TRAF2 and TRAF3 play essential and nonredundant roles in the alternative NF-B pathway PMID: 18997792
    49. the degradation of NIK occurs upon assembly of a regulatory complex through TRAF3 recruitment of NIK and TRAF2 recruitment of cIAP1 and cIAP2. PMID: 18997794
    50. TRAF2 plays a critical role in TNF signaling by directing the IKK complex to the membrane, promoting TRAF2 K63-linked ubiquitination, and positioning the IKKalpha and IKKbeta chains with the TAK1/TAB kinase. PMID: 19150425

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  • 亚细胞定位：
    Cytoplasm.
  • 蛋白家族：
    TNF receptor-associated factor family, A subfamily
  • 组织特异性：
    Isoform 1 and isoform 2 are expressed in spleen, adipose tissues, skeletal muscles, thymus, testis, heart, lung, brain. Isoform 2 is very weakly expressed in heart, lung and brain.
  • 数据库链接：

    KEGG: mmu:22030

    STRING: 10090.ENSMUSP00000028311

    UniGene: Mm.3399