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  4. Recombinant Mouse TIR domain-containing adapter molecule 1 (Ticam1), partial

Recombinant Mouse TIR domain-containing adapter molecule 1 (Ticam1), partial

  • 货号：
    CSB-YP767320MO1
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP767320MO1
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP767320MO1-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP767320MO1
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP767320MO1
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Ticam1; Trif; TIR domain-containing adapter molecule 1; TICAM-1; Toll-interleukin-1 receptor domain-containing adapter protein inducing interferon beta; TIR domain-containing adapter protein inducing IFN-beta
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Involved in innate immunity against invading pathogens. Adapter used by TLR3, TLR4 (through TICAM2) and TLR5 to mediate NF-kappa-B and interferon-regulatory factor (IRF) activation, and to induce apoptosis. Ligand binding to these receptors results in TRIF recruitment through its TIR domain. Distinct protein-interaction motifs allow recruitment of the effector proteins TBK1, TRAF6 and RIPK1, which in turn, lead to the activation of transcription factors IRF3 and IRF7, NF-kappa-B and FADD respectively. Phosphorylation by TBK1 on the pLxIS motif leads to recruitment and subsequent activation of the transcription factor IRF3 to induce expression of type I interferon and exert a potent immunity against invading pathogens. Component of a multi-helicase-TICAM1 complex that acts as a cytoplasmic sensor of viral double-stranded RNA (dsRNA) and plays a role in the activation of a cascade of antiviral responses including the induction of proinflammatory cytokines.
  • 基因功能参考文献：
    1. Findings confirm that signalling through MyD88 is the primary driver for Lipopolysaccharide-dependent NF-kappaB translocation to the nucleus. The pattern of NF-kappaB dynamics in TRIF-deficient cells does not, however, directly reflect the kinetics of TNFalpha promoter activation, supporting the concept that TRIF-dependent signalling plays an important role in the transcription of this cytokine. PMID: 28469251
    2. Mice with myeloid cell specific TIR-domain-containing adapter-inducing interferon-beta (TRIF) knockout showed a trend towards accelerated onset of STZ-induced diabetes, while TRIF deficiency resulted in reduced IDO expression in vivo and in vitro. MyD88 signaling in myeloid cells is a critical pathogenic factor in autoimmune diabetes, which is antagonized by TRIF-dependent responses. PMID: 29522531
    3. Findings suggest that Toll/IL-1R domain-containing adapter-inducing IFN-beta may be involved in the epileptogenesis of temporal lobe epilepsy, which would make it a potential therapeutic target for the treatment of epilepsy. PMID: 28867282
    4. The TLR3/TICAM-1 pathway inhibits polyposis through suppression of c-Myc expression and supports long survival in Apc (Min/+) mice. PMID: 29041928
    5. Versican is produced by Trif- and type I interferon-dependent signaling in macrophages and contributes to fine control of innate immunity in lungs PMID: 28912382
    6. TRIF contributes to murine host defense during the initial response to leptospiral infection PMID: 27259371
    7. work reveals that simulated microgravity promotes the apoptotic response through a combined modulation of the Uev1A/TICAM/TRAF/NF-kappaB-regulated apoptosis and the p53/PCNA- and ATM/ATR-Chk1/2-controlled DNA-damage response pathways. PMID: 26887372
    8. Results show that Monophosphoryl lipid A-induced neutrophil and monocyte recruitment, expansion of bone marrow progenitors and augmentation of neutrophil adhesion molecule expression are regulated by both the MyD88- and TRIF-dependent pathways. PMID: 27354411
    9. Upon stimulation with poly(I:C), malaria parasite-infected red blood cells (iRBCs), or vesicular stomatitis virus (VSV), FOSL1 "translocated" from the nucleus to the cytoplasm, where it inhibited the interactions between TNF receptor-associated factor 3 (TRAF3), TIR domain-containing adapter inducing IFN-beta (TRIF), and Tank-binding kinase 1 (TBK1) via impairing K63-linked polyubiquitination of TRAF3 and TRIF. PMID: 28049150
    10. These results suggested the importance of TRIF in TLR2 mediated foam cell formation via inflammatory mediators, including MCP-1. PMID: 27572666
    11. DENV replication and IFNalpha/beta, TNF-alpha, IL-12 and IL-18 in infected cultures at 24h were found. All of these parameters were significantly decreased after TRIF, MYD88 or NF-kB inhibition PMID: 27575706
    12. The s demonstrate that, in addition to MyD88, Yersinia pseudotuberculosis inhibits TRIF signaling through the type III secretion system effector YopJ. PMID: 26651944
    13. Results show that toll/IL-1 domain-containing adaptor inducing IFN-beta (TRIF) is essential for Toll-like receptors TLR3- and TLR4-mediated innate immune responses in peritoneal mesothelial cells (PMCs). PMID: 26579632
    14. Data show that annexin A2 (AnxA2) directly exerted negative regulation of inflammatory responses through Toll-like receptor 4 (TLR4)-initiated TRAM protein-TRIF protein pathway occurring on endosomes. PMID: 26527544
    15. ProTalpha preconditioning-induced prevention of retinal ischemic damage is mediated by selective activation of the TIR-domain-containing adapter-inducing interferon-beta interferon regulatory factor 3 pathway downstream of toll-like receptor 4 in microglia PMID: 26364961
    16. signaling through TRIF is important for the inflammatory response of AngII-induced abdominal aortic aneurysm formation PMID: 26100679
    17. analysis of how MyD88 and TRIF affect TLR4 activation PMID: 26175492
    18. In mice with nonfunctional TRIF (Trif(mut) mice), Ang II-induced hypertension and cardiac hypertrophy were abrogated, and proinflammatory gene expression in heart and kidneys was unchanged or decreased. These results indicate that Ang II induces activation of a proinflammatory innate immune response, causing hypertension and cardiac hypertrophy, which require functional adaptor protein TRIF-mediated pathways. PMID: 26195481
    19. Ang II induces activation of a proinflammatory innate immune response, causing hypertension and cardiac hypertrophy. These effects require functional adaptor protein TRIF-mediated pathways. PMID: 26195481
    20. Data indicate that Toll-like receptor 4 (TLR4) endocytosis and the TIR-domain-containing adapter-inducing IFN-beta (TRIF)-signaling pathway in macrophages during endotoxin tolerance in the absence of cluster of differentiation 14 (CD14). PMID: 26106158
    21. A MAVS/TICAM-1-independent interferon-inducing pathway contributes to regulation of hepatitis B virus replication PMID: 25115498
    22. signaling is involved in the antitumor activity of poly I:C PMID: 24192491
    23. Trif was mainly present in the ganglion cell layer(GCL)after ischemia-reperfusion (IR) injury. Retinas of TrifKO mice had a significantly reduced neurotoxic pro-inflammatory response and significantly increased survival of the GCL neurons after IR. PMID: 24754835
    24. Mice with single or double deficiencies in these MyD88 and TRIF had reduced responses, based on finding fewer GFPhi Retinal dendritic cell in injured retinas post-optic nerve crush injury. PMID: 25116321
    25. Microbiota-derived compounds drive steady-state granulopoiesis via MyD88/TICAM signaling. PMID: 25305320
    26. The results identify TRIF signaling as a truly homeostatic pathway to maintain intestinal epithelial barrier function revealing fundamental differences in the innate immune signaling between mucosal homeostasis and tissue repair. PMID: 25210121
    27. Caspase-1 directly cleaved TRIF to diminish TRIF-mediated signaling, resulting in inhibition of autophagy and in reduced type I interferon production in Pseudomonas aeruginosa infected macrophages. PMID: 24528867
    28. The role of TRIF signaling is early rejection of allogeneic bone marrow transplantation by macrophages or NK Cells. PMID: 23146386
    29. TLR3-Trif signaling represents an injurious pathway during myocardial ischemia reperfusion injury. Extracellular RNA released during I/R may contribute to myocardial inflammation and infarction. PMID: 24390148
    30. These observations suggest a critical role for the MyD88 pathway in initiating neuropathic pain, but a distinct role for the TRIF pathway and interferon in regulating neuropathic pain phenotypes in male mice. PMID: 24321498
    31. Data indicate that Toll/IL-IR domain-containing adapter-inducing IFN-beta (TRIF) signaling is essential for TLR4/LPS-driven IgE class switching. PMID: 24532577
    32. Data indicate that signalling through the TRIF and TRAM pathways downstream of TLR3 and TLR4 in bone-marrow cells promote the development of atherosclerosis. PMID: 23417039
    33. MyD88 and TRIF must be expressed in the same cell for the in vivo T(H)1-skewing adjuvant activity, indicating these two signaling pathways cooperate on an intracellular level. PMID: 23716300
    34. protective properties of Hsp70 are TLR4 adapter Trif dependent and MyD88 independent. PMID: 23817427
    35. A novel, gender specific protective role was found for TRIF (and CX3CR1) signaling in a model of neonatal hypoxic-ischemic brain injury. PMID: 23843525
    36. these data suggest that hyperlipidemia resulting in endogenous activation of the TRIF signaling pathway from TLR4 leads to pro-atherogenic events. PMID: 22637968
    37. ATO prevents NO production by inhibiting the TIR-domain-containing adaptor protein inducing IFN-beta (TRIF)-dependent pathway. PMID: 23106696
    38. TLR2-mediated TRIF signaling is dependent on the presence of another adaptor molecule, MyD88. PMID: 23166161
    39. WWP2 negatively regulates TLR3-mediated innate immune and inflammatory responses by targeting TRIF for ubiquitination and degradation. PMID: 23479606
    40. Steady-state neutrophil homeostasis is dependent on TLR4/TRIF signaling. PMID: 23223360
    41. TLR4- and TRIF-induced caspase-11 synthesis is critical for noncanonical Nlrp3 inflammasome activation in macrophages infected with enteric pathogens. PMID: 22898816
    42. Secretion of interleukin (IL)-1beta upon bacterial RNA stimulation is independent of TRIF. PMID: 22634614
    43. This study provided the evidence that TRIF-mediated signaling plays an unexpected role in axonal debris clearance by microglia, thereby facilitating a more permissive environment for axonal outgrowth. PMID: 22649252
    44. the D299G polymorphism compromises recruitment of MyD88 and TRIF to TLR4 without affecting TLR4 expression, TLR4-MD2 interaction, or LPS binding, suggesting that it interferes with TLR4 dimerization PMID: 22474023
    45. Data show that toll-like receptors (TLRs)-TRIF signalling activates eIF2B GEF through PP2A-mediated serine dephosphorylation of the eIF2B varepsilon-subunit. PMID: 22231169
    46. TLR4-TRIF axis has an important role in stimulating protective innate immunity against H5N1 influenza A virus infection. PMID: 22031950
    47. Findings strongly support the concept that LMP7/MECL-1 proteasomes subunits actively function to regulate LPS-induced NO production by affecting the TRIF/TRAM pathway. PMID: 21455681
    48. disruption of MyD88 or TRIF signaling does not confer protection in brain ischemia PMID: 21376021
    49. results demonstrate that TLR4 signaling, via the downstream MyD88 and TRIF molecules, exerts a differential regulation on the CD4(+) T cell response to Porphyromonas gingivalis hemagglutinin B antigen PMID: 21498664
    50. Toll/IL-1R domain-containing adapter (TICAM1-) induces interferon-beta in wound healing by regulating chemokine production and recruitment of myeloid cells to the wound for tissue repair. PMID: 21317384

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  • 亚细胞定位：
    Cytoplasm, cytosol. Cytoplasmic vesicle, autophagosome. Mitochondrion.
  • 数据库链接：

    KEGG: mmu:106759

    STRING: 10090.ENSMUSP00000055104

    UniGene: Mm.203952