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  4. Recombinant Mouse THO complex subunit 5 homolog (Thoc5)

Recombinant Mouse THO complex subunit 5 homolog (Thoc5)

  • 货号：
    CSB-YP803894MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP803894MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP803894MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP803894MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP803894MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Thoc5
  • Uniprot No.：
  • 别名：
    Thoc5; Fmip; Kiaa0983; THO complex subunit 5 homolog; Fms-interacting protein; FMIP
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-683
  • 氨基酸序列
    SSESSKKRK PKVIRSDGTP TEGKRNRSDT EQEGKYYSEE AEVDLRDPGR DYELYKYTCQ ELQRLMAEIQ DLKSKGSKDV AIEIEERRIQ SCVHFMTLKK LNRLAHIRLK KGRDQTHEAK QKVDAYHLQL QNLLYEVMHL QKEITKCLEF KSKHEEIDLV SLEEFYSEAP PSISKAEITM GDPHQQTLAR LDWELEQRKR LAEKYRECLS NKEKILKEIE VKRDYLSSLQ PRLNSIMQAS LPVQEYLFMP FDQAHKQYET ARHLPPPLYV LFVQATAYGQ ACDKTLSVAI EGSVDEAKAL FKPPEDSQDD ESDSDAEEEQ TTKRRRPTLG VQLDDKRKEM LKRHPLSVLL DLKCKDNSVL HLTFYYLMNL NIMTVKAKVT TAVELITPIS AGDLLSPDSV LSCLYPGDHG KKTPNPANQY QFDKVGILTL RDYVLELGHP YLWVQKLGGL HFPKEQPQQT VMPDHSQSAS HMETTMKLLK TRVQSRLALH KQFASLEHGI VPVTSDCQDL FPAKVVSRLV KWVIITHEDY MELHFTKDIV EAGLAGDTNL YYLALIERGT AKLQAAVVLN PGYSSIPPVF RLCLNWKGEK TNSNDDNIRA MESEVNVCYK ELCGPRPSHQ LLTNQLQRLC VLLDVYLETE SHDDSFEGPK EFPQEKMCLR LFRGPSRMKP FKYNHPQGFF SHR
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Acts as component of the THO subcomplex of the TREX complex which is thought to couple mRNA transcription, processing and nuclear export, and which specifically associates with spliced mRNA and not with unspliced pre-mRNA. TREX is recruited to spliced mRNAs by a transcription-independent mechanism, binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export to the cytoplasm via the TAP/NFX1 pathway. THOC5 in conjunction with ALYREF/THOC4 functions in NXF1-NXT1 mediated nuclear export of HSP70 mRNA; both proteins enhance the RNA binding activity of NXF1 and are required for NXF1 localization to the nuclear rim. Involved in transcription elongation and genome stability. Involved in alternative polyadenylation site choice by recruiting CPSF6 to 5' region of target genes; probably mediates association of the TREX and CFIm complexes.; Regulates the expression of myeloid transcription factors CEBPA, CEBPB and GAB2 by enhancing the levels of phosphatidylinositol 3,4,5-trisphosphate. May be involved in the differentiation of granulocytes and adipocytes. Essential for hematopoietic primitive cell survival and plays an integral role in monocytic development.
  • 基因功能参考文献：
    1. These results imply that murine leukemia virus requires UAP56, THOC5 and THOC7, in addition to NXF1, for nuclear export of viral transcripts. PMID: 24618812
    2. THOC5, a member of the mRNA export complex, contributes to processing of a subset of wingless/integrated (Wnt) target mRNAs and integrity of the gut epithelial barrier. PMID: 24267292
    3. Regulation of immediate-early gene response by THOC5, a member of mRNA export complex contributes to the M-CSF-induced macrophage differentiation. PMID: 24157873
    4. THOC5 may be required for mRNA export under stress and/or upon signaling-induced conditions PMID: 21525145
    5. THOC5/Fms interacting protein is an essential element in the maintenance of hematopoiesis, and depletion of THOC5/Fms interacting protein causes the down-regulation of THOC1, which may contribute to altered THO complex function and cell death PMID: 20051105
    6. Wild-type FMIP and FMIPSS5,6AA inhibited M-CSF-mediated survival signaling, while FMIPSS5,6EE-expressing cells survived and differentiated into macrophages more efficiently than wild-type cells in the presence of M-CSF or TPA PMID: 15221008
    7. FMIP influences the differentiation of C/EBP alpha gene during adipocyte differentiation of multipotent C2C12 cells. PMID: 16909111

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  • 亚细胞定位：
    Nucleus. Cytoplasm.
  • 蛋白家族：
    THOC5 family
  • 组织特异性：
    Ubiquitously expressed, with highest levels in testis, liver and heart.
  • 数据库链接：

    KEGG: mmu:107829

    STRING: 10090.ENSMUSP00000045580

    UniGene: Mm.28969