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  4. Recombinant Mouse Src kinase-associated phosphoprotein 1 (Skap1)

Recombinant Mouse Src kinase-associated phosphoprotein 1 (Skap1)

  • 货号：
    CSB-YP662390MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP662390MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP662390MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP662390MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP662390MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Skap1; Scap1; Src kinase-associated phosphoprotein 1; Src family-associated phosphoprotein 1
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-355
  • 氨基酸序列
    MQAVALPEEI CWLLEDTEDF LAEGLQNENL SPGAQDQRAH ILRGFQQIKS RYCWDFQPQG GDLGQDGSDD NLSGTHGPPL TSEASFWSDY QDEGIEDILR GAQELDSVIK QGYLEKKSKD HSFFGSEWQK RWCVISRGLF LYYANEKSKQ PKGTFLIKGY SVRMAPHLRK DSKKESCFEL ISQDRRSYEF TASSPAEARD WVDQISFLLK DLSSLTIPFE EEEEEEEEEE KEEEEMYNDV DGFDSPRSGS QCRAMALPEP TEKEEDIYEV LPDDDDLEED TCGAHRRRVD YADYYQGLWD CHGDQPDELS FQRGDLIRIL SKEYNMYGWW VGELNSVIGI VPKDYLTTAF EMEGI
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Positively regulates T-cell receptor signaling by enhancing the MAP kinase pathway. Required for optimal conjugation between T-cells and antigen-presenting cells by promoting the clustering of integrin ITGAL on the surface of T-cells. May be involved in high affinity immunoglobulin epsilon receptor signaling in mast cells.
  • 基因功能参考文献：
    1. SKAP1 can affect the function of talin in T-cells needed for optimal T-cell/dendritic cell conjugation PMID: 26905930
    2. this study shows that Skap1-deficient (skap1-/-) mice are highly resistant to the induction of collagen-induced arthritis PMID: 27181093
    3. In this study, the s identified that the ADAP-SKAP55 signaling module reduced CD8(+) cytotoxic T lymphocyte cytotoxicity and enhanced PD-1 expression in a Fyn-, Ca(2+)-, and NFATc1-dependent manner. PMID: 25851535
    4. Data suggest that the presence or absence of associated SKAP55 defines functionally distinct pools of ADAP. PMID: 21525391
    5. HPK1 associates with SKAP1 to negatively regulate Rap1-mediated B-lymphocyte adhesion. PMID: 20824186
    6. SKAP1 is dispensable for both CXCL12 and CCL21 induced T-cell migration. PMID: 19883688
    7. findings define a T cell receptor "inside-out" pathway via N-SKAP1-C-RapL that regulates T cell adhesion, motility, and arrest times with dendritic cells in lymph nodes. PMID: 20346707
    8. Results identify a clear effector role for SKAP-55 in LFA-1 adhesion in peripheral T cells and demonstrate that dependency on SKAP-55 and ADAP differs among T cells and differs with the strength of the TCR signal. PMID: 17646386

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  • 亚细胞定位：
    Cytoplasm. Nucleus. Cell membrane.
  • 蛋白家族：
    SKAP family
  • 数据库链接：

    KEGG: mmu:78473

    STRING: 10090.ENSMUSP00000099443

    UniGene: Mm.395840