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  4. Recombinant Mouse Sphingosine kinase 2 (Sphk2)

Recombinant Mouse Sphingosine kinase 2 (Sphk2)

  • 货号：
    CSB-YP022565MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP022565MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP022565MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP022565MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP022565MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Sphk2; Sphingosine kinase 2; SK 2; SPK 2; EC 2.7.1.91
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-617
  • 氨基酸序列
    MAPPPLLPVA ASTPILHGEF GSYPANGPRF ALTLTTQALH IQRLRPKPEA RPRDGLVSLD EVSGCGTLQS RSPEDTAAYF CIYTYPRGRR GGRRRATRTF RADGATTYEE NRAEAQRWAT ALTCLLRGVP LSGDQEITPE LLPRKPRLLI LVNPFGGRGL AWQRCMDHVV PMISEAGLSF NLIQTERQNH ARELVQGLSL SEWEGIVTVS GDGLLYEVLN GLLDRPDWED AVRMPIGVLP CGSGNALAGA VNHHGGFEQV VGVDLLLNCS LLLCRGGSHP LDLLSVTLAS GSRCFSFLSV AWGFLSDVDI HSERFRALGS ARFTLGAVLG LASLHTYRGR LSYLPATTEP ALPIPGHSLP RAKSELVLAP APAPAATHSP LHRSVSDLPL PLPQPALVSP GSPEPLPDLS LNGGGPELTG DWGGAGDAPL SPDPLLPSSP NALKTAQLSP IAEGPPEMPA SSGFLPPTHS APEASTWGPV DHLLPPLGSP LPQDWVTIEG EFVLMLGILP SHLCADLMAA PHARFDDGVV HLCWVRSGIS RAALLRILLA MEHGNHFSLG CPHLGYAAAR AFRLEPLTPR GLLTVDGELV EYGPIQAQVH PGLATLLTGP AGQKPQA
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Catalyzes the phosphorylation of sphingosine to form sphingosine-1-phosphate (SPP), a lipid mediator with both intra- and extracellular functions. Also acts on D-erythro-dihydrosphingosine, D-erythro-sphingosine and L-threo-dihydrosphingosine. Binds phosphoinositides. In contrast to prosurvival SPHK1, has a positive effect on intracellular ceramide levels, inhibits cells growth and enhances apoptosis. In mitochondria, is important for cytochrome-c oxidase assembly and mitochondrial respiration. The SPP produced in mitochondria binds PHB2 and modulates the regulation via PHB2 of complex IV assembly and respiration. In nucleus, plays a role in epigenetic regulation of gene expression. Interacts with HDAC1 and HDAC2 and, through SPP production, inhibits their enzymatic activity, preventing the removal of acetyl groups from lysine residues with histones. Upregulates acetylation of histone H3-K9, histone H4-K5 and histone H2B-K12. In nucleus, may have an inhibitory effect on DNA synthesis and cell cycle. In mast cells, is the main regulator of SPP production which mediates calcium influx, NF-kappa-B activation, cytokine production, such as TNF and IL6, and degranulation of mast cells. In dopaminergic neurons, is involved in promoting mitochondrial functions regulating ATP and ROS levels. Also involved in the regulation of glucose and lipid metabolism.
  • 基因功能参考文献：
    1. these data demonstrate that SK-2 exerts an antiproliferative and apoptosis-sensitizing effect in renal mesangial cells which suggests that selective inhibitors of SK-2 may promote proliferation and reduce apoptosis PMID: 29991026
    2. Anti-inflammatory (M2) macrophages decreased in obstructed Sphk2-/- kidneys, as did mRNA levels of inflammatory cytokines suggesting an anti-inflammatory bias in the absence of Sphk2. IL-4 or IL-13 treatment showed that macrophages lacking Sphk2 polarized more efficiently to the M2 phenotype than wild type. Sphk2 is a novel driver of the pro-inflammatory macrophage phenotype. PMID: 29518138
    3. This study demonstrated that SphK2 is responsible for the vast majority of S1P synthesis in the mouse brain, and plays a role in freezing responses as evaluated in the fear conditioning paradigm. PMID: 28625547
    4. sphingosine kinase-2 plays an important role in kidney fibrogenesis by modulating transforming growth factor-beta signaling PMID: 28807595
    5. Taken together, we conclude that antamiR-613 protects neuronal cells from OGDR probably via inducing SphK2 expression. PMID: 28916166
    6. data suggest that in vitro inhibition of SK-2, can compromise the integrity of the EC monolayer PMID: 26822263
    7. Results show that Sphk2/S1P has an important role to play in the survival of the dopaminergic neurons, in the pathogenesis of Parkinson's disease PMID: 25637806
    8. Demonstrate that Sphk2 is the major Sphk isoform responsible for the generation of S1P in platelets and plays a pivotal intrinsic role in the control of platelet activation. PMID: 26129975
    9. transcriptionally up-regulated by acute endoplasmic reticulum stress through activation of ATF4 and improves perturbed hepatic glucose and fatty acid metabolism PMID: 25808625
    10. These results strongly indicate that autophagy is involved in isoflurane preconditioning both in vivo and in vitro and that SPK2 contributes to preconditioning-induced autophagy, possibly by disrupting the Beclin 1/Bcl-2 interaction. PMID: 24928515
    11. Sphk2 provides the source of intracellular S1P that controls thrombopoiesis, which is associated with Src family kinases expression and activity in megakaryocytes. PMID: 23775711
    12. sphingosine kinase-2 has a role in a murine model of lupus nephritis PMID: 23301082
    13. Applying genetic and pharmacological approaches, we demonstrate that neuronal SPK2 isoform plays an important role in cerebral preconditioning. PMID: 21980199
    14. Junctional protein regulation by sphingosine kinase 2 contributes to blood-brain barrier protection in hypoxic preconditioning-induced cerebral ischemic tolerance. PMID: 22314269
    15. Deletion of the SphK2 gene sensitizes the myocardium to IR injury and diminishes the protective effect of IPC. PMID: 21904650
    16. the mitochondrial Sphingosine-1-phosphate produced by SphK2 is required for the downstream protective modulation of mitochondrial permeability transition pore as an effector of preconditioning protection PMID: 22002221
    17. This suggests that SphK2 activity is an important endogenous protective mechanism in cerebral ischemia. PMID: 21872577
    18. Cleavage of sphingosine kinase 2 by caspase-1 provokes its release from apoptotic cells. PMID: 20197547
    19. SK2 feeds blood-borne sphingosine 1-phosphate (S1P) into the degradation pathway of cellular S1P and plays a pivotal role in regulation and transport of S1P in blood and lymphoid tissues. PMID: 20220090
    20. the apoptotic effect of SphK2 might be because of its putative BH3 domain PMID: 12835323
    21. SPHK2 associates with the interleukin (IL)-12 receptor beta 1 cytoplasmic region and appears to be involved in the positive modulation of IL-12 signaling, especially IL-12-induced interferon-gamma production. PMID: 12874225
    22. a functional nuclear localization signal is mapped to the N-terminal region of SPHK2 PMID: 12954646
    23. the N-terminal-extended form of sphingosine kinase 2 has a role in serum-dependent regulation of cell proliferation and apoptosis PMID: 16103110
    24. FTY720 does not induce lymphopenia in SPHK2-deficient mice but is required to maintain FTY720 phosphate levels in vivo. PMID: 16223773
    25. Sphingosine kinase-null mice exhibited a deficiency of S1P which severely disturbed neurogenesis, including neural tube closure, and angiogenesis and caused embryonic lethality. PMID: 16314531
    26. Sphk2 regulates IL-2 pathways in T cells PMID: 17947634
    27. constitutive expression of SphK2 may contribute to reduced ischemia-reperfusion injury of the kidney, and its absence may enhance injury due to increased neutrophil infiltration and S1P(3) activation PMID: 18971925
    28. These findings indicate hypoxia-sensitive increases in SphK2 activity may serve as a proximal trigger that ultimately leads to sphingosine-1-phosphate-mediated alterations in gene expression that promote the ischemia-tolerant phenotype. PMID: 19644058

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  • 亚细胞定位：
    Lysosome membrane. Cytoplasm. Cell membrane. Endoplasmic reticulum. Nucleus. Mitochondrion inner membrane.
  • 组织特异性：
    Expressed in heart, brain, liver, kidney and testis. Expressed by mast cells (at protein level). In the substantia nigra, expressed by dopaminergic neurons (at protein level).
  • 数据库链接：

    KEGG: mmu:56632

    STRING: 10090.ENSMUSP00000072615

    UniGene: Mm.24222