Your Good Partner in Biology Research

  1. 首页
  2. 蛋白
  3. 重组蛋白
  4. Recombinant Mouse Sorting nexin-27 (Snx27)

Recombinant Mouse Sorting nexin-27 (Snx27)

  • 货号：
    CSB-YP663660MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP663660MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP663660MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP663660MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP663660MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Snx27
  • Uniprot No.：
  • 别名：
    Snx27; Kiaa0488; Sorting nexin-27
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-539
  • 氨基酸序列
    MADEDGEGIH PSAPHRNGGG GGGSGLHCAG NGGGGGGGPR VVRIVKSESG YGFNVRGQVS EGGQLRSING ELYAPLQHVS AVLPGGAADR AGVRKGDRIL EVNGVNVEGA THKQVVDLIR AGEKELILTV LSVPPHEADN LDPSDDSLGQ SFYDYTEKQA VPISVPTYKH VEQNGEKFVV YNVYMAGRQL CSKRYREFAI LHQNLKREFA NFTFPRLPGK WPFSLSEQQL DARRRGLEEY LEKVCSIRVI GESDIMQEFL SESDENYNGV SDVELRVALP DGTTVTVRVK KNSTTDQVYQ AIAAKVGMDS TTVNYFALFE VINHSFVRKL APNEFPHKLY VQNYTSAVPG TCLTIRKWLF TTEEEVLLND NDLAVTYFFH QAVDDVKKGY IKAEEKSYQL QKLHEQRKMV MYLNMLRTCE GYNEIIFPHC ACDSRRKGHV ITAISITHFK LHACTEEGQL ENQVIAFEWD EMQRWDTDEE GMAFCFEYAR GEKKPRWVKI FTPYFNYMHE CFERVFCELK WRKENIFQMA RSQQRDVAT
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Involved in the retrograde transport from endosome to plasma membrane, a trafficking pathway that promotes the recycling of internalized transmembrane proteins. Following internalization, endocytosed transmembrane proteins are delivered to early endosomes and recycled to the plasma membrane instead of being degraded in lysosomes. SNX27 specifically binds and directs sorting of a subset of transmembrane proteins containing a PDZ-binding motif at the C-terminus: following interaction with target transmembrane proteins, associates with the retromer complex, preventing entry into the lysosomal pathway, and promotes retromer-tubule based plasma membrane recycling. SNX27 also binds with the WASH complex. Interacts with membranes containing phosphatidylinositol-3-phosphate (PtdIns(3P)). May participate in establishment of natural killer cell polarity. Recruits CYTIP to early endosomes.
  • 基因功能参考文献：
    1. Results show a crucial role of SNX27 in modulating GPR17 levels by means of a post-translational mechanism and highlights the relationship between the trafficking of the receptor through the endomembrane system and oligodendrocyte differentiation; and provide novel evidence of impairment of GPR17 expression and oligodendrocyte maturation in a mouse model of Down syndrome that is characterized by SNX27 down-regulation. PMID: 27270750
    2. we anticipate that future study will determine whether modulation of a SNX27/Notch/gamma-secretase pathway can also be of therapeutic interest to congenital hydrocephalus PMID: 27974614
    3. We identify binding and trafficking interactions between two of these factors, SORLA and SNX27, and demonstrate that SNX27 can direct trafficking of SORLA and the Abeta precursor APP to the cell surface to limit the production of Abeta. Diversion APP to the cell surface through modulation of this molecular complex may represent a complimentary strategy for future development in AD treatment. PMID: 27466343
    4. A critical role for SNX27-retromer mediated transport of PTHR in normal bone development. PMID: 26912788
    5. a function of SNX27 in regulating beta-amyloid (Abeta) generation by modulating gamma-secretase activity. PMID: 25437557
    6. VPS26A binding increases the affinity of the SNX27 PDZ domain for PDZ- binding motifs by an order of magnitude, revealing cooperativity in cargo selection. PMID: 25136126
    7. SNX27-dependent mechanism of inhibitory plasticity in DA neurons is a potential intervention point for disorders of dopamine neurons. PMID: 24811384
    8. beta1-adrenergic receptor recycles via a membranous organelle, recycling endosome, by binding with sorting nexin27. PMID: 23780416
    9. SNX27 associates with ZO-2 and modulates the epithelial tight junction. PMID: 23826934
    10. a model by which SNX27 regulates trafficking of beta-Pix to focal adhesions and thereby influences cell motility. PMID: 21926430
    11. indicate that SNX27 may function to regulate endocytosis and/or endosomal sorting of NR2C PMID: 21300787
    12. SNX27 and NHERF specifically interact with the 5-HT4a receptor splice variant PMID: 15466885
    13. These data support a previously unreported role for diacylglycerol kinase zeta in the modulation of membrane trafficking, which may also help to define SNX27 function. PMID: 17351151
    14. This study shows that SNX27 promotes the endosomal movement of Kir3 (or GIRK) channels, leading to reduced surface expression, increased degradation and smaller Kir3 potassium currents. PMID: 17828261

    显示更多

    收起更多

  • 亚细胞定位：
    Early endosome membrane; Peripheral membrane protein. Cytoplasm, cytosol.
  • 组织特异性：
    Expressed in cells of hematopoietic origin.
  • 数据库链接：

    KEGG: mmu:76742

    STRING: 10090.ENSMUSP00000102904

    UniGene: Mm.28160