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  4. Recombinant Mouse Sestrin-3 (Sesn3)

Recombinant Mouse Sestrin-3 (Sesn3)

  • 货号：
    CSB-YP863465MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP863465MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP863465MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP863465MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP863465MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Sesn3; Sestrin-3
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-492
  • 氨基酸序列
    MNRGGSSASA SANYLLCTNC RKVLRKDKRI RVSQPLTRGP SAFIPEKEVV QANTADERTN FLVEEYSTSG RLDNITQVMS LHTQYLESFL RSQFYMLRMD GPLPLPDRHY IAIMAAARHQ CSYLINMHVD EFLKTGGIAE WLNGLEYVPQ RLRNLNEINK LLAHRPWLIT KEHIQKLVKT GENNWSLPEL VHAVVLLAHY HALASFVFGS GINPERDPGI ANGFRLISVS SFCVCDLAND NSIENTSLAG SNFGIVDSLG ELEALMERMK RLQEDREDDE TTREEMTTRF EKEKKESLFV VPGETLHAFP HSDFEDDVIV TADVSRYIED PSFGYEDFAR RGEEHLPTFR AQDYTWENHG FSLVNRLYSD IGHLLDEKFR MVYNLTYNTM ATHEDVDTTT LRRALFNYVH CMFGIRYDDY DYGEVNQLLE RSLKVYIKTV TCYPERTTKR MYDSYWRQFT HSEKVHVNLL LMEARMQAEL LYALRAITRH LT
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    May function as an intracellular leucine sensor that negatively regulates the TORC1 signaling pathway. May also regulate the insulin-receptor signaling pathway through activation of TORC2. This metabolic regulator may also play a role in protection against oxidative and genotoxic stresses. May prevent the accumulation of reactive oxygen species (ROS) through the alkylhydroperoxide reductase activity born by the N-terminal domain of the protein.
  • 基因功能参考文献：
    1. Sesn3 liver-specific knockout mice exhibit insulin resistance and glucose intolerance. Sesn3 interacts with and activates mTORC2 and subsequently stimulates Akt phosphorylation at Ser473. PMID: 25377878
    2. Study found that Sestrins (1,2 and 3) can inhibit mTORC1 signaling in the absence of AMPK or TSC2. Surprisingly, when coexpressed with a GTP-bound constitutively active form of RagB (RagBQ99L), Sestrins potentiate mTORC1 signaling in the absence of amino acids. PMID: 25259925
    3. Suggest that inhibitory effect of ethanol on Sesn3 may play an important role in the development of ethanol-induced fatty liver. PMID: 24833709
    4. Concomitant ablation of Sesn2 and Sesn3 provokes hepatic mTORC1-S6K activation and insulin resistance even in the absence of nutritional overload and obesity. PMID: 22958918
    5. FoxOs inhibit mTORC1 and activate Akt by inducing the expression of Sestrin3 and Rictor PMID: 20412774

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  • 亚细胞定位：
    Cytoplasm.
  • 蛋白家族：
    Sestrin family
  • 组织特异性：
    Detected in liver and skeletal muscles.
  • 数据库链接：

    KEGG: mmu:75747

    STRING: 10090.ENSMUSP00000034507

    UniGene: Mm.325126