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  4. Recombinant Mouse Serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform (Ppp3ca)

Recombinant Mouse Serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform (Ppp3ca)

  • 货号：
    CSB-YP350527MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP350527MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP350527MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP350527MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP350527MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Ppp3ca; Calna; Serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform; EC 3.1.3.16; CAM-PRP catalytic subunit; Calmodulin-dependent calcineurin A subunit alpha isoform; CNA alpha
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-521
  • 氨基酸序列
    SEPKAIDPK LSTTDRVVKA VPFPPSHRLT AKEVFDNDGK PRVDILKAHL MKEGRLEESV ALRIITEGAS ILRQEKNLLD IDAPVTVCGD IHGQFFDLMK LFEVGGSPAN TRYLFLGDYV DRGYFSIECV LYLWALKILY PKTLFLLRGN HECRHLTEYF TFKQECKIKY SERVYDACMD AFDCLPLAAL MNQQFLCVHG GLSPEINTLD DIRKLDRFKE PPAYGPMCDI LWSDPLEDFG NEKTQEHFTH NTVRGCSYFY SYPAVCDFLQ HNNLLSILRA HEAQDAGYRM YRKSQTTGFP SLITIFSAPN YLDVYNNKAA VLKYENNVMN IRQFNCSPHP YWLPNFMDVF TWSLPFVGEK VTEMLVNVLN ICSDDELGSE EDGFDGATAA ARKEVIRNKI RAIGKMARVF SVLREESESV LTLKGLTPTG MLPSGVLSGG KQTLQSATVE AIEADEAIKG FSPQHKITSF EEAKGLDRIN ERMPPRRDAM PSDANLNSIN KALASETNGT DSNGSNSSNI Q
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Calcium-dependent, calmodulin-stimulated protein phosphatase which plays an essential role in the transduction of intracellular Ca(2+)-mediated signals. Many of the substrates contain a PxIxIT motif and/or a LxVP motif. In response to increased Ca(2+) levels, dephosphorylates and activates phosphatase SSH1 which results in cofilin dephosphorylation. In response to increased Ca(2+) levels following mitochondrial depolarization, dephosphorylates DNM1L inducing DNM1L translocation to the mitochondrion. Dephosphorylates heat shock protein HSPB1. Dephosphorylates and activates transcription factor NFATC1. Dephosphorylates and inactivates transcription factor ELK1. Dephosphorylates DARPP32. May dephosphorylate CRTC2 at 'Ser-171' resulting in CRTC2 dissociation from 14-3-3 proteins.
  • 基因功能参考文献：
    1. calcineurin is a key regulator of Wnt5a-induced AQP2 activation without affecting intracellular cAMP level and PKA activity. PMID: 27892464
    2. these results demonstrate that increased susceptibility to Aspergillus fumigatus is dependent on calcineurin signaling in myeloid cells PMID: 27301880
    3. the CaN-Nfat pathway regulates development and function of IFN-gamma-producing gammadelta T cells. PMID: 28062573
    4. the expression of the full-length 60-kDa calcineurin protein is down-regulated due to over-activated calpain that cleaves calcineurin to form a 45-kDa fragment. PMID: 27171192
    5. Calcineurin inhibitor tacrolimus ameliorated proteinuria in nephrotic model by inhibiting the redistribution of CN-A and nephrin at the slit diaphragm. PMID: 27009276
    6. global deficiency of catalytic subunit Ppp3cb, and tissue-specific ablation of regulatory subunit Ppp3r1 from skeletal muscle, but not adipose tissue or liver, led to protection from high-fat-diet-induced obesity PMID: 26411342
    7. Overexpression of the phosphatase calcineurin A prevents the development of a capillary/myocyte mismatch despite progressive myocardial hypertrophy. PMID: 26031702
    8. Catalytic subunits of the phosphatase calcineurin interact with NF-kappaB-inducing kinase (NIK) and attenuate NIK-dependent gene expression PMID: 26029823
    9. Data indicate that calcineurin (CnAalpha-/- or CnAbeta-/-) is required for pathological but not compensatory renal hypertrophy. PMID: 25287476
    10. We also confirmed that overexpression of RCAN1-1L could inhibit the transcriptional activation of an NFAT-dependent promoter in response to PMA and ionomycin by inhibiting Calcineurin activity in HEK293T cells PMID: 25193101
    11. Calcineurin and its regulator, RCAN1, confer time-of-day changes in susceptibility of the heart to ischemia/reperfusion. PMID: 24838101
    12. KSR2 deficiency affects stromal interaction molecule 1 (STIM1)/ORAI1 puncta formation, which is correlated with cytoskeleton disorganization. PMID: 24672054
    13. Data indicate that reduction in connexin43 (Cx43) and sodium channel NaV1.5 expression coincided with overexpression of transgene calcineurin A (CnA) and hypertrophy development and preceded significant presence of fibrosis. PMID: 24498049
    14. Calcineurin regulates Nirr1 expression in a cell-autonomous, neural activity-dependent manner. PMID: 24291696
    15. Muscle-specific RING finger 1 negatively regulates pathological cardiac hypertrophy through downregulation of calcineurin A. PMID: 24526353
    16. Defects in renal development and function persist in adult calcineurin A subunit alpha -/- mice including a significant decrease in glomerular filtration rate and an increase in blood urea nitrogen levels. PMID: 23638102
    17. In calcineurin knock-out mice, an impairment of synaptic vesicle release was found in prefrontal cortex. PMID: 23825400
    18. Syndecan-4 is essential for development of concentric myocardial hypertrophy via stretch-induced activation of the calcineurin-NFAT pathway PMID: 22164265
    19. Calcineurin is essential for gonadotropin-releasing hormone-induced expression of TORC1 and Nur77. PMID: 21986493
    20. These data suggest that Gq stimulates CN and promotes podocyte apoptosis both in vitro and in vivo. PMID: 21622531
    21. Results indicate that myospryn functions as a novel inhibitor of the calcineurin signaling pathway in skeletal muscle. PMID: 21427212
    22. Inhibition by IL-2 occurred via a natural killer cell-independent component of the calcineurin pathway, and CD94/NKG2A induction was markedly enhanced in the presence of calcineurin blockers PMID: 21540458
    23. calcineurin has a role in digestion and protein trafficking PMID: 21435446
    24. Normal, opposing circadian oscillations in calcineurin-dependent activities and phosphorylation of proteins that regulate contractility are disrupted in heart failure. PMID: 21233454
    25. in response to transverse aortic constriction, time-dependent changes in Akt/GSK3beta and calcineurin/NFAT signalling occur with varying amplitudes that structure the progression towards HF as a 3-stage process. PMID: 21076497
    26. identification of Cryab as a downstream effector of calcineurin-related protection against myocardial apoptosis PMID: 20937869
    27. Calcineurin protects the heart in a murine model of dilated cardiomyopathy PMID: 19854199
    28. calcineurin has a role in the heart and in cardiac disease PMID: 19879877
    29. calcineurin activation in skeletal muscle increases mitochondrial oxidative function and energy substrate storage, which contributes to enhanced endurance exercise performance PMID: 19861587
    30. Plasma renin concentrations were not different in wild-type, calcineurin A-alpha, or A-beta knockout mice. PMID: 19907416
    31. calcineurin Aalpha activity is required for the normal differentiation and survival of epidermal cells PMID: 19626032
    32. Calcineurin Aalpha is important for mature T cell function, but has a limited role in thymocyte development. PMID: 11981809
    33. a trimeric-interaction among calcineurin, FKBP12, and RyR is important for the regulation of the RyR channel activity and may play an important role in the Ca(2+) signaling of muscle contraction and relaxation PMID: 12414688
    34. calcineurin expression is important during myogenesis and fiber-type switching, but not for muscle growth in response to hypertrophic stimuli PMID: 15082723
    35. absence of CnA-alpha but not CnA-beta leads to a defect in normal maturation of the NZ and glomeruli, alterations in the cell cycle, and impaired kidney function PMID: 15509543
    36. CnAalpha is required for normal intracellular trafficking of AQP2 and loss of calcineurin protein or activity disrupts AQP2 function. PMID: 16735444
    37. Increased calcineurin activity may confer functional benefits to dystrophic skeletal muscles. PMID: 16793906
    38. Calcineurin A (CnA) alpha (-/-) mice display renal hypoplasia associated with the ectopic expression of p27. PMID: 17072859
    39. calcineurin activation can confer structural and functional benefits to regenerating skeletal muscles, which may be mediated in part by differential expression of myoregulatory factors PMID: 17475677
    40. Calcineurin acts as a signal to up-regulate the fast-oxidative MyHC2a gene and to down-regulate the faster MyHC2x and MyHC2b genes in a manner that appears to be NFAT-independent. PMID: 17587059
    41. PPP3ca was highly expressed during the stages of the seminiferous epithelium most sensitive to low doses of cadmium chloride. PMID: 17785681
    42. Cholecystokinin activates NFATs in a calcineurin-dependent manner, both in vitro and in vivo. PMID: 17978097
    43. a decrease in fiber cross-sectional area, normalized force was lower in fast- and slow-twitch muscles of mdx ppp3ca knockout than mdx mice PMID: 18199592
    44. Haploinsufficiency of calcineurin A-alpha causes upregulation of TGF-beta which contributes to chronic inflammation and formation of tertiary lymphoid organs. PMID: 19129256
    45. Data suggest that the regulation of cytoglobin transcription by calcineurin-dependent transcription factors suggests that cytoglobin may have a functional role in calcium-dependent events accompanying cardiac remodeling. PMID: 19203999

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  • 亚细胞定位：
    Cytoplasm. Cell membrane; Peripheral membrane protein. Cell membrane, sarcolemma. Cytoplasm, myofibril, sarcomere, Z line. Cell projection, dendritic spine.
  • 蛋白家族：
    PPP phosphatase family, PP-2B subfamily
  • 数据库链接：

    KEGG: mmu:19055

    STRING: 10090.ENSMUSP00000053101

    UniGene: Mm.331389