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  4. Recombinant Mouse Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform (Ppp2r1a)

Recombinant Mouse Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform (Ppp2r1a)

  • 货号：
    CSB-YP758842MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP758842MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP758842MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP758842MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP758842MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Ppp2r1aSerine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform; PP2A subunit A isoform PR65-alpha; PP2A subunit A isoform R1-alpha
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-589
  • 氨基酸序列
    AAADGDDSL YPIAVLIDEL RNEDVQLRLN SIKKLSTIAL ALGVERTRSE LLPFLTDTIY DEDEVLLALA EQLGTFTTLV GGPEYVHCLL PPLESLATVE ETVVRDKAVE SLRAISHEHS PSDLEAHFVP LVKRLAGGDW FTSRTSACGL FSVCYPRVSS AVKAELRQYF RNLCSDDTPM VRRAAASKLG EFAKVLELDN VKSEIIPMFS NLASDEQDSV RLLAVEACVN IAQLLPQEDL EALVMPTLRQ AAEDKSWRVR YMVADKFTEL QKAVGPEITK TDLVPAFQNL MKDCEAEVRA AASHKVKEFC ENLSADCREN VIMTQILPCI KELVSDANQH VKSALASVIM GLSPILGKDN TIEHLLPLFL AQLKDECPEV RLNIISNLDC VNEVIGIRQL SQSLLPAIVE LAEDAKWRVR LAIIEYMPLL AGQLGVEFFD EKLNSLCMAW LVDHVYAIRE AATSNLKKLV EKFGKEWAHA TIIPKVLAMS GDPNYLHRMT TLFCINVLSE VCGQDITTKH MLPTVLRMAG DPVANVRFNV AKSLQKIGPI LDNSTLQSEV KPILEKLTQD QDVDVKYFAQ EALTVLSLA
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    The PR65 subunit of protein phosphatase 2A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit. Upon interaction with GNA12 promotes dephosphorylation of microtubule associated protein TAU/MAPT. Required for proper chromosome segregation and for centromeric localization of SGO1 in mitosis.
  • 基因功能参考文献：
    1. oocyte-specific deletion of Ppp2r1a led to severe female subfertility without affecting follicle survival, growth, and ovulation. PMID: 24899574
    2. PP2A-A alpha transcriptional regulation is mediated by multiple factors including AP-2alpha, CREB, ETS-1, and SP-1 PMID: 19750005
    3. protein phosphatase 2A overexpression in NIH 3T3 cells PMID: 11665638
    4. T-cell receptor (TCR)-dependent tyrosine phosphorylation may be the mechanism by which the regulatory subunit of PP2A prevents the inhibitory function of CTLA-4, before TCR-CTLA-4 coligation. PMID: 11994459
    5. PP-2A regulation of paxillin phosphorylation may have a role in controlling tumor cell adherence and motility. PMID: 12198769
    6. PP2A may contribute to melanoma cell radioresistance: the truncated isoform of the PP2A B56gamma regulatory subunit reduces irradiation-induced Mdm2 phosphorylation PMID: 15024700
    7. PP2A has a fundamental role in cardiac function PMID: 15247211
    8. response of endothelial cells to the tumor-derived motility-stimulatory factors PGE2/TGFbeta involved a decline in the activity of PP2A, a loss of PP2A co-precipitation with PTEN, and an increase in the PTEN serine phosphorylation level. PMID: 15516833
    9. heat treatment inactivates PP2A, which may subsequently cause ERK activation and decreases melanin synthesis in melanocytes PMID: 15894174
    10. Results indicate that polyamines regulate protein phosphatase 2A (PP2A)activity, and inhibition of PP2A in response to polyamine depletion increases levels of Bad and Bcl-2 proteins and prevents cytochrome c release, caspase-9, and caspase-3 activation. PMID: 15994315
    11. Pim on PP2A activity may mediate the levels of c-Myc and the phosphorylation of proteins needed for increased protein synthesis, which could have a significant impact on tumor growth PMID: 16123140
    12. PP2A plays a positive rather than a negative role in the regulation of IKKbeta PMID: 16126728
    13. the carboxy-terminal leucine L309 of the PP2A catalytic subunit determines PP2A heterotrimer composition in vivo. PP2A subunit composition plays a crucial role in regulating cell adhesion and as a consequence in the development of the Harderian gland. PMID: 16679006
    14. PP2A-mediated dephosphorylation of BCL-2 is required to protect BCL-2 from proteasome-dependent degradation, affecting resistance to ER stress PMID: 16717086
    15. PP2A is involved in the post-transcriptional regulation of tumor necrosis factor-alpha. PMID: 17170118
    16. Akt dephosphorylation was mediated by ceramide-induced PKCzeta-Akt association and PP2A activation in Leishmania donovani-infected macrophages. PMID: 17220321
    17. Based on crystal structures of Galpha(12) and PP2A Aalpha, a model describing the binding surfaces and potential mechanisms of Galpha(12)-mediated PP2A activation is presented. PMID: 17303700
    18. protein phosphatase 2a recruitment to I-kappaB kinase gamma/NF-kappaB essential modulator is regulated by heptad repeats, which are targeted by HTLV-I tax PMID: 17314097
    19. Suppression of PP-2A damages intracellular transport and leads to cell degeneration, and the memory deficits induced by suppression of the phosphatases. PMID: 17472709
    20. Results describe the crystal structure of full-length SV40 small t antigen in complex with protein phosphatase 2A A subunit at 3.1 A resolution. PMID: 17608567
    21. These results indicate a role for GSK-3beta in ceramide-induced apoptosis, in which GSK-3beta acts downstream of PP2A and the PI 3-kinase-Akt pathway, and upstream of caspase-2 and caspase-8. PMID: 17666435
    22. C3G triggers PP2A activation and binding to MEK and ERK at the subcortical actin cytoskeleton, thus favouring ERK dephosphorylation. PMID: 17825818
    23. Polyoma small T uses PP2A to promote phosphorylation of Akt on both T308 & S473, resulting in a different pattern of phosphorylation of Akt substrates & shifting Akt from a proapoptotic to an antiapoptotic mode. PMID: 18006659
    24. These data show that A(2A)R and A(1)R regulate PP2A activity, thus suggesting an important mechanism for modulating myocardial contractility. PMID: 18181173
    25. PP2A is a physiologic phosphatase of FOXO1. PMID: 18211894
    26. a role for the demethylated form of PP2A in maintenance of enzyme function and phosphorylation networks in vivo. PMID: 18596935
    27. Nitration in PP2Ac is correlated with PP2A activation that mediates endothelial barrier dysfunction. PMID: 18791203
    28. B56epsilon is alternatively translated adds a new level of regulation to PP2A holoenzymes PMID: 19129191
    29. Inhibition of serine/threonine phosphatase PP2A enhances cancer chemotherapy by blocking DNA damage induced defense mechanisms. PMID: 19564615
    30. Knockdown of PP2A inhibits forskolin-induced nuclear translocation of HDAC4 and attenuates the ability of this signaling molecule to repress collagen X expression in chondrocytes. PMID: 19704004
    31. Study describes a novel Akt-dependent Pp2a activity that acts at least partly through Jun to affect initial barrier formation during late embryonic epidermal development. PMID: 19762425

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  • 亚细胞定位：
    Cytoplasm. Nucleus. Chromosome, centromere. Lateral cell membrane. Cell projection, dendrite.
  • 蛋白家族：
    Phosphatase 2A regulatory subunit A family
  • 数据库链接：

    KEGG: mmu:51792

    STRING: 10090.ENSMUSP00000007708

    UniGene: Mm.294138